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Literature summary extracted from

  • Pinkse, M.W.; Rijkers, D.T.; Dostmann, W.R.; Heck, A.J.
    Mode of action of cGMP-dependent protein kinase-specific inhibitors probed by photoaffinity cross-linking mass spectrometry (2009), J. Biol. Chem., 284, 16354-16368.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.12 cGMP
-
 Bos taurus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.12 bovine PKG type Ialpha is expressed in SF9 insect cells Bos taurus

Protein Variants

EC Number Protein Variants Comment Organism
2.7.11.12 PKG DELTA77 N-terminal deletion mutant, which lacks the dimerization domain Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.12 LRKKKKKH
-
 Bos taurus
2.7.11.12 TQAKRKKALAMA-NH2
-
 Bos taurus
2.7.11.12 TTYDFIASGRTGRRNAIHD-NH2
-
 Bos taurus
2.7.11.12 YGRKKRRQRRRPP
-
 Bos taurus
2.7.11.12 YGRKKRRQRRRPPLRKKKKKH
-
 Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.12 cytosol
-
 Bos taurus 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.12 76000
-
 monomer Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.12 ATP + a protein Bos taurus
-
 ADP + a phosphoprotein
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.12 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.12
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.12 cerebellum
-
 Bos taurus
-
2.7.11.12 lung
-
 Bos taurus
-
2.7.11.12 smooth muscle
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.12 ATP + a protein
-
 Bos taurus ADP + a phosphoprotein
-
 ?
2.7.11.12 additional information peptide substrates with a primary amino acid sequence motif RRX(S/T)X are in general recognized by PKG Bos taurus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.11.12 homodimer 2 * 76000 Bos taurus

Synonyms

EC Number Synonyms Comment Organism
2.7.11.12 cGMP-dependent protein kinase
-
 Bos taurus
2.7.11.12 PKG
-
 Bos taurus
2.7.11.12 PKG type Ialpha
-
 Bos taurus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.11.12 0.0000125
-
 YGRKKRRQRRRPPLRKKKKKH
-
 Bos taurus
2.7.11.12 0.00082
-
 LRKKKKKH
-
 Bos taurus
2.7.11.12 0.0011
-
 YGRKKRRQRRRPP
-
 Bos taurus
2.7.11.12 0.0075
-
 TQAKRKKALAMA-NH2
-
 Bos taurus
2.7.11.12 0.15
-
 TTYDFIASGRTGRRNAIHD-NH2
-
 Bos taurus