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Literature summary extracted from

  • Charriere, F.; ODonoghue, P.; Helgadottir, S.; Marechal-Drouard, L.; Cristodero, M.; Horn, E.K.; Soell, D.; Schneider, A.
    Dual targeting of a tRNAAsp requires two different aspartyl-tRNA synthetases in Trypanosoma brucei (2009), J. Biol. Chem., 284, 16210-16217.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.12 the sequence corresponding to the carboxyl-terminal Ty1-tagged Tb-AspRS1 is cloned into a derivative of pLew-100 to allow tetracycline-inducible expression of the tagged protein in transgenic cell lines Trypanosoma brucei
6.1.1.12 the sequence corresponding to the carboxyl-terminal Ty1-tagged Tb-AspRS1 is cloned into a derivative of pLew-100 to allow tetracycline-inducible expression of the tagged protein in transgenic cell lines, into the vector pET15b for expression in Escherichia coli BL21-CodonPlus DE3-RIL cells Trypanosoma brucei

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.1.1.12 RNAi
-
 Trypanosoma brucei

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.1.1.12 cytosol
-
 Trypanosoma brucei 5829
-
6.1.1.12 mitochondrion
-
 Trypanosoma brucei 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.12 60800
-
 theoretical Trypanosoma brucei
6.1.1.12 62900
-
 theoretical Trypanosoma brucei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.12 ATP + L-aspartate + tRNAAsp Trypanosoma brucei
-
 AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.12 Trypanosoma brucei Q38CB4
-
-
6.1.1.12 Trypanosoma brucei Q585M3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.12 by Ni2+-nitrilotriacetic acid chromatography, cytosolic and mitochondrial fractions of Trypanosoma brucei are prepared Trypanosoma brucei

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.1.1.12 additional information
-
 100% aminoacylation of cytosolic tRNA, 4% aminoacylation of mitochondrial tRNA Trypanosoma brucei
6.1.1.12 additional information
-
 100% aminoacylation of cytosolic tRNA, 90% aminoacylation of mitochondrial tRNA Trypanosoma brucei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.12 ATP + L-aspartate + tRNAAsp
-
 Trypanosoma brucei AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.12 Aspartyl-tRNA synthetase
-
 Trypanosoma brucei
6.1.1.12 AspRS
-
 Trypanosoma brucei
6.1.1.12 Tb-AspRS1
-
 Trypanosoma brucei
6.1.1.12 Tb-AspRS2
-
 Trypanosoma brucei

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.12 ATP
-
 Trypanosoma brucei