Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Sobhany, M.; Kakuta, Y.; Sugiura, N.; Kimata, K.; Negishi, M.
    The chondroitin polymerase K4CP and the molecular mechanism of selective bindings of donor substrates to two active sites (2008), J. Biol. Chem., 283, 32328-32333.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.175 expressed in Escherichia coli BL21(DE3) cells Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.175 0.005895
-
 UDP-N-acetyl-alpha-D-glucosamine wild type enzyme, in 25 mM HEPES (pH 7.5), 100 mM NaCl, 20 mM MnCl2, 1 mM CaCl2, at 30°C Escherichia coli
2.4.1.175 0.005895
-
 UDP-N-acetyl-beta-D-galactosamine wild type enzyme, in 25 mM HEPES (pH 7.5), 100 mM NaCl, 20 mM MnCl2, 1 mM CaCl2, at 30°C Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.175 Escherichia coli
-
-
-
2.4.1.175 Escherichia coli K4
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.175 glutathione-Sepharose resin column chromatography Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.175 additional information no hydrolysis activity is observed with UDP-N-glucuronic acid Escherichia coli ?
-
 ?
2.4.1.175 additional information no hydrolysis activity is observed with UDP-N-glucuronic acid Escherichia coli K4 ?
-
 ?
2.4.1.175 UDP-N-acetyl-alpha-D-galactosamine + chondroitin sulfate oligosaccharide
-
 Escherichia coli UDP + N-acetyl-beta-D-galactosaminyl chondroitin sulfate oligosaccharide
-
 ?
2.4.1.175 UDP-N-acetyl-alpha-D-galactosamine + chondroitin sulfate oligosaccharide
-
 Escherichia coli K4 UDP + N-acetyl-beta-D-galactosaminyl chondroitin sulfate oligosaccharide
-
 ?
2.4.1.175 UDP-N-acetyl-alpha-D-glucosamine + chondroitin
-
 Escherichia coli ?
-
 ?
2.4.1.175 UDP-N-acetyl-alpha-D-glucosamine + chondroitin
-
 Escherichia coli K4 ?
-
 ?
2.4.1.175 UDP-N-acetyl-alpha-D-glucosamine + chondroitin sulfate oligosaccharide weak activity Escherichia coli UDP + N-acetyl-beta-D-glucosaminyl chondroitin sulfate oligosaccharide
-
 ?
2.4.1.175 UDP-N-acetyl-alpha-D-glucosamine + chondroitin sulfate oligosaccharide weak activity Escherichia coli K4 UDP + N-acetyl-beta-D-glucosaminyl chondroitin sulfate oligosaccharide
-
 ?
2.4.1.175 UDP-N-acetyl-beta-D-galactosamine + chondroitin
-
 Escherichia coli ?
-
 ?
2.4.1.175 UDP-N-acetyl-beta-D-galactosamine + chondroitin
-
 Escherichia coli K4 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.175 chondroitin polymerase
-
 Escherichia coli
2.4.1.175 K4CP
-
 Escherichia coli