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Literature summary extracted from

  • Kaminski, P.A.; Dacher, P.; Dugue, L.; Pochet, S.
    In vivo reshaping the catalytic site of nucleoside 2-deoxyribosyltransferase for dideoxy- and didehydronucleosides via a single amino acid substitution (2008), J. Biol. Chem., 283, 20053-20059.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.4.2.6 A15C kcat is diminished as compared to wild-type Limosilactobacillus fermentum
2.4.2.6 A15S activity with 2'-deoxycytidine and adenine as substrates is closer to that of the wild-type enzyme than that of the A15T mutant, kcat is diminished as compared to wild-type Limosilactobacillus fermentum
2.4.2.6 A15T mutation allows conversion of the nucleoside 2'deoxyribosyltransferase activity to a nucleoside 2',3'-dideoxy- or 2',3'-didehydro,2',3'-dideoxyribosyltransferase activity, kcat is diminished as compared to wild-type Limosilactobacillus fermentum
2.4.2.6 A15V can not improve 2',3'-dideoxyribose transfer reaction, kcat is diminished as compared to wild-type Limosilactobacillus fermentum
2.4.2.6 G9C can not improve 2',3'-dideoxyribose transfer reaction, kcat is diminished as compared to wild-type Lactobacillus leichmannii
2.4.2.6 G9S mutation allows conversion of the nucleoside 2'deoxyribosyltransferase activity to a nucleoside 2',3'-dideoxy- or 2',3'-didehydro,2',3'-dideoxyribosyltransferase activity, kcat is diminished as compared to wild-type Lactobacillus leichmannii
2.4.2.6 G9T Ser remains a better substituent for Gly-9 than Thr, kcat is diminished as compared to wild-type Lactobacillus leichmannii
2.4.2.6 G9W can not improve 2',3'-dideoxyribose transfer reaction, kcat is diminished as compared to wild-type Lactobacillus leichmannii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.6 0.25
-
 2'-deoxycytidine wild-type Lactobacillus leichmannii
2.4.2.6 0.3
-
 2'-deoxycytidine wild-type Limosilactobacillus fermentum
2.4.2.6 0.3
-
 2'-deoxycytidine mutant G9S Lactobacillus leichmannii
2.4.2.6 0.38
-
 2',3'-dideoxycytidine wild-type Limosilactobacillus fermentum
2.4.2.6 0.39
-
 2'-deoxycytidine mutant A15T Limosilactobacillus fermentum
2.4.2.6 0.8
-
 2',3'-dideoxycytidine mutant A15T Limosilactobacillus fermentum
2.4.2.6 0.92
-
 2',3'-dideoxycytidine mutant G9S Lactobacillus leichmannii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.6 18080
-
 native enzyme, mass spectrometry Lactobacillus leichmannii
2.4.2.6 18110
-
 mutant, mass spectrometry Lactobacillus leichmannii
2.4.2.6 18900
-
 native enzyme, mass spectrometry Limosilactobacillus fermentum
2.4.2.6 18930
-
 mutant, mass spectrometry Limosilactobacillus fermentum

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.6 Lactobacillus leichmannii Q9RSV5
-
-
2.4.2.6 Limosilactobacillus fermentum Q6YNI5
-
-
2.4.2.6 Limosilactobacillus fermentum PAK9 Q6YNI5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.6
-
 Limosilactobacillus fermentum
2.4.2.6
-
 Lactobacillus leichmannii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.6 0.01
-
 wild-type, with 2',3'-dideoxyguanosine and thymine as substrates Limosilactobacillus fermentum
2.4.2.6 0.01
-
 wild-type, with 2',3'-dideoxyguanosine and thymine as substrates Lactobacillus leichmannii
2.4.2.6 0.01
-
 wild-type, with 2',3'-dideoxyinosine and adenine as substrates Limosilactobacillus fermentum
2.4.2.6 0.01
-
 wild-type, with 2',3'-dideoxyinosine and adenine as substrates Lactobacillus leichmannii
2.4.2.6 0.01
-
 wild-type, with 2',3'didehydro-2',3'-dideoxythymidine and adenine as substrates Lactobacillus leichmannii
2.4.2.6 0.01
-
 wild-type, with 2'-deoxycytidine and hypoxanthine as substrates Lactobacillus leichmannii
2.4.2.6 0.017
-
 wild-type, with 2',3'-dideoxythymidine and adenine as substrates Lactobacillus leichmannii
2.4.2.6 0.02
-
 mutant G9S, with 2'-deoxyinosine and adenine as substrates Limosilactobacillus fermentum
2.4.2.6 0.03
-
 wild-type, with 2',3'-dideoxythymidine and adenine as substrates Limosilactobacillus fermentum
2.4.2.6 0.036
-
 wild-type, with 2',3'didehydro-2',3'-dideoxythymidine and adenine as substrates Limosilactobacillus fermentum
2.4.2.6 0.04
-
 mutant G9S, with 2',3'-dideoxyinosine and adenine as substrates Lactobacillus leichmannii
2.4.2.6 0.04
-
 wild-type, with 2',3'-dideoxycytidine and hypoxanthine as substrates Lactobacillus leichmannii
2.4.2.6 0.05
-
 wild-type, with 2',3'-dideoxycytidine and hypoxanthine as substrates Limosilactobacillus fermentum
2.4.2.6 0.056
-
 wild-type, with 2',3'-dideoxyuridine and cytosine as substrates Lactobacillus leichmannii
2.4.2.6 0.06
-
 mutant G9S, with 2',3'-dideoxyinosine and adenine as substrates Limosilactobacillus fermentum
2.4.2.6 0.06
-
 wild-type, with 2'-deoxycytidine and hypoxanthine as substrates Limosilactobacillus fermentum
2.4.2.6 0.19
-
 wild-type, with 2',3'-dideoxyuridine and cytosine as substrates Limosilactobacillus fermentum
2.4.2.6 0.2
-
 mutant G9S, with 2',3'didehydro-2',3'-dideoxythymidine and adenine as substrates Lactobacillus leichmannii
2.4.2.6 0.25
-
 mutant G9S, with 3'-dideoxythymidine and adenine as substrates Lactobacillus leichmannii
2.4.2.6 0.4
-
 mutant G9S, with 2',3'-dideoxyguanosine and thymine as substrates Lactobacillus leichmannii
2.4.2.6 0.47
-
 mutant G9S, with 2'-deoxyinosine and adenine as substrates Lactobacillus leichmannii
2.4.2.6 0.5
-
 mutant G9S, with 3'-dideoxythymidine and adenine as substrates Limosilactobacillus fermentum
2.4.2.6 0.57
-
 mutant G9S, with 2'-deoxyguanosine and thymine Limosilactobacillus fermentum
2.4.2.6 0.6
-
 mutant G9S, with 2',3'-dideoxyguanosine and thymine as substrates Limosilactobacillus fermentum
2.4.2.6 0.8
-
 mutant G9S, with 2',3'-dideoxycytidine and hypoxanthine as substrates Lactobacillus leichmannii
2.4.2.6 1.04
-
 mutant G9S, with 2'-deoxythymidine and adenine as substrates Lactobacillus leichmannii
2.4.2.6 1.08
-
 wild-type, with 2'-deoxyinosine and adenine as substrates Limosilactobacillus fermentum
2.4.2.6 1.1
-
 mutant G9S, with 2'-deoxyguanosine and thymine Lactobacillus leichmannii
2.4.2.6 1.25
-
 mutant G9S, with 2'-deoxycytidine and hypoxanthine as substrates Lactobacillus leichmannii
2.4.2.6 1.29
-
 mutant G9S, with 2',3'-dideoxycytidine and adenine as substrates Limosilactobacillus fermentum
2.4.2.6 1.3
-
 mutant G9S, with 2',3'-dideoxyuridine and cytosine as substrates Lactobacillus leichmannii
2.4.2.6 1.6
-
 mutant G9S, with 2',3'-dideoxyuridine and cytosine as substrates Limosilactobacillus fermentum
2.4.2.6 1.9
-
 mutant G9S, with 2'-deoxythymidine and adenine as substrates Limosilactobacillus fermentum
2.4.2.6 3.1
-
 mutant G9S, with 2'-deoxyuridine and cytosine as substrates Lactobacillus leichmannii
2.4.2.6 3.6
-
 wild-type, with 2'-deoxyinosine and adenine as substrates Lactobacillus leichmannii
2.4.2.6 4.95
-
 wild-type, with 2'-deoxyguanosine and thymine Limosilactobacillus fermentum
2.4.2.6 5.2
-
 wild-type, with 2'-deoxyguanosine and thymine Lactobacillus leichmannii
2.4.2.6 6
-
 mutant G9S, with 2',3'-dideoxycytidine and adenine as substrates Lactobacillus leichmannii
2.4.2.6 6.8
-
 mutant G9S, with 2',3'-dideoxycytidine and hypoxanthine as substrates Limosilactobacillus fermentum
2.4.2.6 6.9
-
 wild-type, with 2',3'-dideoxycytidine and adenine as substrates Limosilactobacillus fermentum
2.4.2.6 7.1
-
 mutant G9S, with 2'-deoxycytidine and hypoxanthine as substrates Limosilactobacillus fermentum
2.4.2.6 9.28
-
 mutant G9S, with 2',3'didehydro-2',3'-dideoxythymidine and adenine as substrates Limosilactobacillus fermentum
2.4.2.6 9.3
-
 mutant G9S, with 2'-deoxycytidine and adenine as substrates Limosilactobacillus fermentum
2.4.2.6 11.9
-
 wild-type, with 2'-deoxythymidine and adenine as substrates Lactobacillus leichmannii
2.4.2.6 12.7
-
 mutant G9S, with 2'-deoxyuridine and cytosine as substrates Limosilactobacillus fermentum
2.4.2.6 20.1
-
 wild-type, with 2'-deoxythymidine and adenine as substrates Limosilactobacillus fermentum
2.4.2.6 27.1
-
 mutant G9S, with 2'-deoxycytidine and adenine as substrates Lactobacillus leichmannii
2.4.2.6 29
-
 wild-type, with 2',3'-dideoxycytidine and adenine as substrates Lactobacillus leichmannii
2.4.2.6 40.2
-
 wild-type, with 2'-deoxyuridine and cytosine as substrates Limosilactobacillus fermentum
2.4.2.6 42.5
-
 wild-type, with 2'-deoxyuridine and cytosine as substrates Lactobacillus leichmannii
2.4.2.6 83.7
-
 wild-type, with 2'-deoxycytidine and adenine as substrates Limosilactobacillus fermentum
2.4.2.6 173
-
 wild-type, with 2'-deoxycytidine and adenine as substrates Lactobacillus leichmannii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.6 2',3'-dideoxycytidine + adenine
-
 Limosilactobacillus fermentum 2',3'-dideoxyadenosine + cytosine
-
 ?
2.4.2.6 2',3'-dideoxycytidine + adenine
-
 Lactobacillus leichmannii 2',3'-dideoxyadenosine + cytosine
-
 ?
2.4.2.6 2',3'-dideoxycytidine + adenine
-
 Limosilactobacillus fermentum PAK9 2',3'-dideoxyadenosine + cytosine
-
 ?
2.4.2.6 2',3'-dideoxycytidine + hypoxanthine
-
 Limosilactobacillus fermentum 2',3'-dideoxyinosine + cytosine
-
 ?
2.4.2.6 2',3'-dideoxycytidine + hypoxanthine
-
 Lactobacillus leichmannii 2',3'-dideoxyinosine + cytosine
-
 ?
2.4.2.6 2',3'-dideoxycytidine + hypoxanthine
-
 Limosilactobacillus fermentum PAK9 2',3'-dideoxyinosine + cytosine
-
 ?
2.4.2.6 2',3'-dideoxyguanosine + thymine
-
 Limosilactobacillus fermentum 2',3'-dideoxythymidine + guanine
-
 ?
2.4.2.6 2',3'-dideoxyguanosine + thymine
-
 Lactobacillus leichmannii 2',3'-dideoxythymidine + guanine
-
 ?
2.4.2.6 2',3'-dideoxyguanosine + thymine
-
 Limosilactobacillus fermentum PAK9 2',3'-dideoxythymidine + guanine
-
 ?
2.4.2.6 2',3'-dideoxyinosine + adenine
-
 Limosilactobacillus fermentum 2',3'-dideoxyadenosine + hypoxanthine
-
 ?
2.4.2.6 2',3'-dideoxyinosine + adenine
-
 Lactobacillus leichmannii 2',3'-dideoxyadenosine + hypoxanthine
-
 ?
2.4.2.6 2',3'-dideoxyinosine + adenine
-
 Limosilactobacillus fermentum PAK9 2',3'-dideoxyadenosine + hypoxanthine
-
 ?
2.4.2.6 2',3'-dideoxythymidine + adenine
-
 Limosilactobacillus fermentum 2',3'-dideoxyadenosine + thymine
-
 ?
2.4.2.6 2',3'-dideoxythymidine + adenine
-
 Lactobacillus leichmannii 2',3'-dideoxyadenosine + thymine
-
 ?
2.4.2.6 2',3'-dideoxythymidine + adenine
-
 Limosilactobacillus fermentum PAK9 2',3'-dideoxyadenosine + thymine
-
 ?
2.4.2.6 2',3'-dideoxyuridine + cytosine
-
 Limosilactobacillus fermentum 2',3'-dideoxycytidine + uracil
-
 ?
2.4.2.6 2',3'-dideoxyuridine + cytosine
-
 Lactobacillus leichmannii 2',3'-dideoxycytidine + uracil
-
 ?
2.4.2.6 2',3'didehydro-2',3'-dideoxythymidine + adenine
-
 Limosilactobacillus fermentum 2',3'didehydro-2',3'-dideoxyadenosine + thymine
-
 ?
2.4.2.6 2',3'didehydro-2',3'-dideoxythymidine + adenine
-
 Lactobacillus leichmannii 2',3'didehydro-2',3'-dideoxyadenosine + thymine
-
 ?
2.4.2.6 2'-deoxycytidine + adenine
-
 Limosilactobacillus fermentum 2'-deoxyadenosine + cytosine
-
 ?
2.4.2.6 2'-deoxycytidine + adenine
-
 Lactobacillus leichmannii 2'-deoxyadenosine + cytosine
-
 ?
2.4.2.6 2'-deoxycytidine + hypoxanthine
-
 Limosilactobacillus fermentum 2'-deoxyinosine + cytosine
-
 ?
2.4.2.6 2'-deoxycytidine + hypoxanthine
-
 Lactobacillus leichmannii 2'-deoxyinosine + cytosine
-
 ?
2.4.2.6 2'-deoxyguanosine + thymine
-
 Limosilactobacillus fermentum 2'-deoxythymidine + guanine
-
 ?
2.4.2.6 2'-deoxyguanosine + thymine
-
 Lactobacillus leichmannii 2'-deoxythymidine + guanine
-
 ?
2.4.2.6 2'-deoxyinosine + adenine
-
 Limosilactobacillus fermentum 2'-deoxyadenosine + hypoxanthine
-
 ?
2.4.2.6 2'-deoxyinosine + adenine
-
 Lactobacillus leichmannii 2'-deoxyadenosine + hypoxanthine
-
 ?
2.4.2.6 2'-deoxythymidine + adenine
-
 Limosilactobacillus fermentum 2'-deoxyadenosine + thymine
-
 ?
2.4.2.6 2'-deoxythymidine + adenine
-
 Lactobacillus leichmannii 2'-deoxyadenosine + thymine
-
 ?
2.4.2.6 2'-deoxyuridine + cytosine
-
 Limosilactobacillus fermentum 2'-deoxycytidine + uracil
-
 ?
2.4.2.6 2'-deoxyuridine + cytosine
-
 Lactobacillus leichmannii 2'-deoxycytidine + uracil
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.2.6 NTD
-
 Limosilactobacillus fermentum
2.4.2.6 NTD
-
 Lactobacillus leichmannii
2.4.2.6 nucleoside 2'-deoxyribosyltransferase
-
 Limosilactobacillus fermentum
2.4.2.6 nucleoside 2'-deoxyribosyltransferase
-
 Lactobacillus leichmannii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.2.6 0.047
-
 2',3'-dideoxycytidine wild-type Limosilactobacillus fermentum
2.4.2.6 2.54
-
 2',3'-dideoxycytidine mutant G9S Lactobacillus leichmannii
2.4.2.6 5.36
-
 2'-deoxycytidine mutant A15T Limosilactobacillus fermentum
2.4.2.6 10.4
-
 2',3'-dideoxycytidine mutant A15T Limosilactobacillus fermentum
2.4.2.6 12.4
-
 2'-deoxycytidine mutant G9S Lactobacillus leichmannii
2.4.2.6 45.1
-
 2'-deoxycytidine wild-type Limosilactobacillus fermentum
2.4.2.6 62.2
-
 2'-deoxycytidine wild-type Lactobacillus leichmannii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.2.6 0.12
-
 2',3'-dideoxycytidine wild-type Limosilactobacillus fermentum
2.4.2.6 9.1
-
 2',3'-dideoxycytidine mutant G9S Lactobacillus leichmannii
2.4.2.6 13
-
 2',3'-dideoxycytidine mutant A15T Limosilactobacillus fermentum
2.4.2.6 14
-
 2'-deoxycytidine mutant A15T Limosilactobacillus fermentum
2.4.2.6 41
-
 2'-deoxycytidine mutant G9S Lactobacillus leichmannii
2.4.2.6 150
-
 2'-deoxycytidine wild-type Limosilactobacillus fermentum
2.4.2.6 250
-
 2'-deoxycytidine wild-type Lactobacillus leichmannii