BRENDA - Enzyme Database show

In vivo reshaping the catalytic site of nucleoside 2-deoxyribosyltransferase for dideoxy- and didehydronucleosides via a single amino acid substitution

Kaminski, P.A.; Dacher, P.; Dugue, L.; Pochet, S.; J. Biol. Chem. 283, 20053-20059 (2008)

Data extracted from this reference:

Engineering
EC Number
Amino acid exchange
Commentary
Organism
2.4.2.6
A15C
kcat is diminished as compared to wild-type
Lactobacillus fermentum
2.4.2.6
A15S
activity with 2'-deoxycytidine and adenine as substrates is closer to that of the wild-type enzyme than that of the A15T mutant, kcat is diminished as compared to wild-type
Lactobacillus fermentum
2.4.2.6
A15T
mutation allows conversion of the nucleoside 2'deoxyribosyltransferase activity to a nucleoside 2',3'-dideoxy- or 2',3'-didehydro,2',3'-dideoxyribosyltransferase activity, kcat is diminished as compared to wild-type
Lactobacillus fermentum
2.4.2.6
A15V
can not improve 2',3'-dideoxyribose transfer reaction, kcat is diminished as compared to wild-type
Lactobacillus fermentum
2.4.2.6
G9C
can not improve 2',3'-dideoxyribose transfer reaction, kcat is diminished as compared to wild-type
Lactobacillus leichmannii
2.4.2.6
G9S
mutation allows conversion of the nucleoside 2'deoxyribosyltransferase activity to a nucleoside 2',3'-dideoxy- or 2',3'-didehydro,2',3'-dideoxyribosyltransferase activity, kcat is diminished as compared to wild-type
Lactobacillus leichmannii
2.4.2.6
G9T
Ser remains a better substituent for Gly-9 than Thr, kcat is diminished as compared to wild-type
Lactobacillus leichmannii
2.4.2.6
G9W
can not improve 2',3'-dideoxyribose transfer reaction, kcat is diminished as compared to wild-type
Lactobacillus leichmannii
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.2.6
0.25
-
2'-deoxycytidine
wild-type
Lactobacillus leichmannii
2.4.2.6
0.3
-
2'-deoxycytidine
wild-type
Lactobacillus fermentum
2.4.2.6
0.3
-
2'-deoxycytidine
mutant G9S
Lactobacillus leichmannii
2.4.2.6
0.38
-
2',3'-dideoxycytidine
wild-type
Lactobacillus fermentum
2.4.2.6
0.39
-
2'-deoxycytidine
mutant A15T
Lactobacillus fermentum
2.4.2.6
0.8
-
2',3'-dideoxycytidine
mutant A15T
Lactobacillus fermentum
2.4.2.6
0.92
-
2',3'-dideoxycytidine
mutant G9S
Lactobacillus leichmannii
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.4.2.6
18080
-
native enzyme, mass spectrometry
Lactobacillus leichmannii
2.4.2.6
18110
-
mutant, mass spectrometry
Lactobacillus leichmannii
2.4.2.6
18900
-
native enzyme, mass spectrometry
Lactobacillus fermentum
2.4.2.6
18930
-
mutant, mass spectrometry
Lactobacillus fermentum
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.2.6
Lactobacillus fermentum
Q6YNI5
-
-
2.4.2.6
Lactobacillus fermentum PAK9
Q6YNI5
-
-
2.4.2.6
Lactobacillus leichmannii
Q9RSV5
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.4.2.6
-
Lactobacillus fermentum
2.4.2.6
-
Lactobacillus leichmannii
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.4.2.6
0.01
-
wild-type, with 2',3'-dideoxyguanosine and thymine as substrates; wild-type, with 2',3'-dideoxyinosine and adenine as substrates
Lactobacillus fermentum
2.4.2.6
0.01
-
wild-type, with 2',3'didehydro-2',3'-dideoxythymidine and adenine as substrates; wild-type, with 2',3'-dideoxyguanosine and thymine as substrates; wild-type, with 2',3'-dideoxyinosine and adenine as substrates; wild-type, with 2'-deoxycytidine and hypoxanthine as substrates
Lactobacillus leichmannii
2.4.2.6
0.017
-
wild-type, with 2',3'-dideoxythymidine and adenine as substrates
Lactobacillus leichmannii
2.4.2.6
0.02
-
mutant G9S, with 2'-deoxyinosine and adenine as substrates
Lactobacillus fermentum
2.4.2.6
0.03
-
wild-type, with 2',3'-dideoxythymidine and adenine as substrates
Lactobacillus fermentum
2.4.2.6
0.036
-
wild-type, with 2',3'didehydro-2',3'-dideoxythymidine and adenine as substrates
Lactobacillus fermentum
2.4.2.6
0.04
-
mutant G9S, with 2',3'-dideoxyinosine and adenine as substrates; wild-type, with 2',3'-dideoxycytidine and hypoxanthine as substrates
Lactobacillus leichmannii
2.4.2.6
0.05
-
wild-type, with 2',3'-dideoxycytidine and hypoxanthine as substrates
Lactobacillus fermentum
2.4.2.6
0.056
-
wild-type, with 2',3'-dideoxyuridine and cytosine as substrates
Lactobacillus leichmannii
2.4.2.6
0.06
-
mutant G9S, with 2',3'-dideoxyinosine and adenine as substrates; wild-type, with 2'-deoxycytidine and hypoxanthine as substrates
Lactobacillus fermentum
2.4.2.6
0.19
-
wild-type, with 2',3'-dideoxyuridine and cytosine as substrates
Lactobacillus fermentum
2.4.2.6
0.2
-
mutant G9S, with 2',3'didehydro-2',3'-dideoxythymidine and adenine as substrates
Lactobacillus leichmannii
2.4.2.6
0.25
-
mutant G9S, with 3'-dideoxythymidine and adenine as substrates
Lactobacillus leichmannii
2.4.2.6
0.4
-
mutant G9S, with 2',3'-dideoxyguanosine and thymine as substrates
Lactobacillus leichmannii
2.4.2.6
0.47
-
mutant G9S, with 2'-deoxyinosine and adenine as substrates
Lactobacillus leichmannii
2.4.2.6
0.5
-
mutant G9S, with 3'-dideoxythymidine and adenine as substrates
Lactobacillus fermentum
2.4.2.6
0.57
-
mutant G9S, with 2'-deoxyguanosine and thymine
Lactobacillus fermentum
2.4.2.6
0.6
-
mutant G9S, with 2',3'-dideoxyguanosine and thymine as substrates
Lactobacillus fermentum
2.4.2.6
0.8
-
mutant G9S, with 2',3'-dideoxycytidine and hypoxanthine as substrates
Lactobacillus leichmannii
2.4.2.6
1.04
-
mutant G9S, with 2'-deoxythymidine and adenine as substrates
Lactobacillus leichmannii
2.4.2.6
1.08
-
wild-type, with 2'-deoxyinosine and adenine as substrates
Lactobacillus fermentum
2.4.2.6
1.1
-
mutant G9S, with 2'-deoxyguanosine and thymine
Lactobacillus leichmannii
2.4.2.6
1.25
-
mutant G9S, with 2'-deoxycytidine and hypoxanthine as substrates
Lactobacillus leichmannii
2.4.2.6
1.29
-
mutant G9S, with 2',3'-dideoxycytidine and adenine as substrates
Lactobacillus fermentum
2.4.2.6
1.3
-
mutant G9S, with 2',3'-dideoxyuridine and cytosine as substrates
Lactobacillus leichmannii
2.4.2.6
1.6
-
mutant G9S, with 2',3'-dideoxyuridine and cytosine as substrates
Lactobacillus fermentum
2.4.2.6
1.9
-
mutant G9S, with 2'-deoxythymidine and adenine as substrates
Lactobacillus fermentum
2.4.2.6
3.1
-
mutant G9S, with 2'-deoxyuridine and cytosine as substrates
Lactobacillus leichmannii
2.4.2.6
3.6
-
wild-type, with 2'-deoxyinosine and adenine as substrates
Lactobacillus leichmannii
2.4.2.6
4.95
-
wild-type, with 2'-deoxyguanosine and thymine
Lactobacillus fermentum
2.4.2.6
5.2
-
wild-type, with 2'-deoxyguanosine and thymine
Lactobacillus leichmannii
2.4.2.6
6
-
mutant G9S, with 2',3'-dideoxycytidine and adenine as substrates
Lactobacillus leichmannii
2.4.2.6
6.8
-
mutant G9S, with 2',3'-dideoxycytidine and hypoxanthine as substrates
Lactobacillus fermentum
2.4.2.6
6.9
-
wild-type, with 2',3'-dideoxycytidine and adenine as substrates
Lactobacillus fermentum
2.4.2.6
7.1
-
mutant G9S, with 2'-deoxycytidine and hypoxanthine as substrates
Lactobacillus fermentum
2.4.2.6
9.28
-
mutant G9S, with 2',3'didehydro-2',3'-dideoxythymidine and adenine as substrates
Lactobacillus fermentum
2.4.2.6
9.3
-
mutant G9S, with 2'-deoxycytidine and adenine as substrates
Lactobacillus fermentum
2.4.2.6
11.9
-
wild-type, with 2'-deoxythymidine and adenine as substrates
Lactobacillus leichmannii
2.4.2.6
12.7
-
mutant G9S, with 2'-deoxyuridine and cytosine as substrates
Lactobacillus fermentum
2.4.2.6
20.1
-
wild-type, with 2'-deoxythymidine and adenine as substrates
Lactobacillus fermentum
2.4.2.6
27.1
-
mutant G9S, with 2'-deoxycytidine and adenine as substrates
Lactobacillus leichmannii
2.4.2.6
29
-
wild-type, with 2',3'-dideoxycytidine and adenine as substrates
Lactobacillus leichmannii
2.4.2.6
40.2
-
wild-type, with 2'-deoxyuridine and cytosine as substrates
Lactobacillus fermentum
2.4.2.6
42.5
-
wild-type, with 2'-deoxyuridine and cytosine as substrates
Lactobacillus leichmannii
2.4.2.6
83.7
-
wild-type, with 2'-deoxycytidine and adenine as substrates
Lactobacillus fermentum
2.4.2.6
173
-
wild-type, with 2'-deoxycytidine and adenine as substrates
Lactobacillus leichmannii
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.2.6
2',3'-dideoxycytidine + adenine
-
704429
Lactobacillus fermentum
2',3'-dideoxyadenosine + cytosine
-
-
-
?
2.4.2.6
2',3'-dideoxycytidine + adenine
-
704429
Lactobacillus leichmannii
2',3'-dideoxyadenosine + cytosine
-
-
-
?
2.4.2.6
2',3'-dideoxycytidine + adenine
-
704429
Lactobacillus fermentum PAK9
2',3'-dideoxyadenosine + cytosine
-
-
-
?
2.4.2.6
2',3'-dideoxycytidine + hypoxanthine
-
704429
Lactobacillus fermentum
2',3'-dideoxyinosine + cytosine
-
-
-
?
2.4.2.6
2',3'-dideoxycytidine + hypoxanthine
-
704429
Lactobacillus leichmannii
2',3'-dideoxyinosine + cytosine
-
-
-
?
2.4.2.6
2',3'-dideoxycytidine + hypoxanthine
-
704429
Lactobacillus fermentum PAK9
2',3'-dideoxyinosine + cytosine
-
-
-
?
2.4.2.6
2',3'-dideoxyguanosine + thymine
-
704429
Lactobacillus fermentum
2',3'-dideoxythymidine + guanine
-
-
-
?
2.4.2.6
2',3'-dideoxyguanosine + thymine
-
704429
Lactobacillus leichmannii
2',3'-dideoxythymidine + guanine
-
-
-
?
2.4.2.6
2',3'-dideoxyguanosine + thymine
-
704429
Lactobacillus fermentum PAK9
2',3'-dideoxythymidine + guanine
-
-
-
?
2.4.2.6
2',3'-dideoxyinosine + adenine
-
704429
Lactobacillus fermentum
2',3'-dideoxyadenosine + hypoxanthine
-
-
-
?
2.4.2.6
2',3'-dideoxyinosine + adenine
-
704429
Lactobacillus leichmannii
2',3'-dideoxyadenosine + hypoxanthine
-
-
-
?
2.4.2.6
2',3'-dideoxyinosine + adenine
-
704429
Lactobacillus fermentum PAK9
2',3'-dideoxyadenosine + hypoxanthine
-
-
-
?
2.4.2.6
2',3'-dideoxythymidine + adenine
-
704429
Lactobacillus fermentum
2',3'-dideoxyadenosine + thymine
-
-
-
?
2.4.2.6
2',3'-dideoxythymidine + adenine
-
704429
Lactobacillus leichmannii
2',3'-dideoxyadenosine + thymine
-
-
-
?
2.4.2.6
2',3'-dideoxythymidine + adenine
-
704429
Lactobacillus fermentum PAK9
2',3'-dideoxyadenosine + thymine
-
-
-
?
2.4.2.6
2',3'-dideoxyuridine + cytosine
-
704429
Lactobacillus fermentum
2',3'-dideoxycytidine + uracil
-
-
-
?
2.4.2.6
2',3'-dideoxyuridine + cytosine
-
704429
Lactobacillus leichmannii
2',3'-dideoxycytidine + uracil
-
-
-
?
2.4.2.6
2',3'didehydro-2',3'-dideoxythymidine + adenine
-
704429
Lactobacillus fermentum
2',3'didehydro-2',3'-dideoxyadenosine + thymine
-
-
-
?
2.4.2.6
2',3'didehydro-2',3'-dideoxythymidine + adenine
-
704429
Lactobacillus leichmannii
2',3'didehydro-2',3'-dideoxyadenosine + thymine
-
-
-
?
2.4.2.6
2'-deoxycytidine + adenine
-
704429
Lactobacillus fermentum
2'-deoxyadenosine + cytosine
-
-
-
?
2.4.2.6
2'-deoxycytidine + adenine
-
704429
Lactobacillus leichmannii
2'-deoxyadenosine + cytosine
-
-
-
?
2.4.2.6
2'-deoxycytidine + hypoxanthine
-
704429
Lactobacillus fermentum
2'-deoxyinosine + cytosine
-
-
-
?
2.4.2.6
2'-deoxycytidine + hypoxanthine
-
704429
Lactobacillus leichmannii
2'-deoxyinosine + cytosine
-
-
-
?
2.4.2.6
2'-deoxyguanosine + thymine
-
704429
Lactobacillus fermentum
2'-deoxythymidine + guanine
-
-
-
?
2.4.2.6
2'-deoxyguanosine + thymine
-
704429
Lactobacillus leichmannii
2'-deoxythymidine + guanine
-
-
-
?
2.4.2.6
2'-deoxyinosine + adenine
-
704429
Lactobacillus fermentum
2'-deoxyadenosine + hypoxanthine
-
-
-
?
2.4.2.6
2'-deoxyinosine + adenine
-
704429
Lactobacillus leichmannii
2'-deoxyadenosine + hypoxanthine
-
-
-
?
2.4.2.6
2'-deoxythymidine + adenine
-
704429
Lactobacillus fermentum
2'-deoxyadenosine + thymine
-
-
-
?
2.4.2.6
2'-deoxythymidine + adenine
-
704429
Lactobacillus leichmannii
2'-deoxyadenosine + thymine
-
-
-
?
2.4.2.6
2'-deoxyuridine + cytosine
-
704429
Lactobacillus fermentum
2'-deoxycytidine + uracil
-
-
-
?
2.4.2.6
2'-deoxyuridine + cytosine
-
704429
Lactobacillus leichmannii
2'-deoxycytidine + uracil
-
-
-
?
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.4.2.6
0.047
-
2',3'-dideoxycytidine
wild-type
Lactobacillus fermentum
2.4.2.6
2.54
-
2',3'-dideoxycytidine
mutant G9S
Lactobacillus leichmannii
2.4.2.6
5.36
-
2'-deoxycytidine
mutant A15T
Lactobacillus fermentum
2.4.2.6
10.4
-
2',3'-dideoxycytidine
mutant A15T
Lactobacillus fermentum
2.4.2.6
12.4
-
2'-deoxycytidine
mutant G9S
Lactobacillus leichmannii
2.4.2.6
45.1
-
2'-deoxycytidine
wild-type
Lactobacillus fermentum
2.4.2.6
62.2
-
2'-deoxycytidine
wild-type
Lactobacillus leichmannii
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
2.4.2.6
A15C
kcat is diminished as compared to wild-type
Lactobacillus fermentum
2.4.2.6
A15S
activity with 2'-deoxycytidine and adenine as substrates is closer to that of the wild-type enzyme than that of the A15T mutant, kcat is diminished as compared to wild-type
Lactobacillus fermentum
2.4.2.6
A15T
mutation allows conversion of the nucleoside 2'deoxyribosyltransferase activity to a nucleoside 2',3'-dideoxy- or 2',3'-didehydro,2',3'-dideoxyribosyltransferase activity, kcat is diminished as compared to wild-type
Lactobacillus fermentum
2.4.2.6
A15V
can not improve 2',3'-dideoxyribose transfer reaction, kcat is diminished as compared to wild-type
Lactobacillus fermentum
2.4.2.6
G9C
can not improve 2',3'-dideoxyribose transfer reaction, kcat is diminished as compared to wild-type
Lactobacillus leichmannii
2.4.2.6
G9S
mutation allows conversion of the nucleoside 2'deoxyribosyltransferase activity to a nucleoside 2',3'-dideoxy- or 2',3'-didehydro,2',3'-dideoxyribosyltransferase activity, kcat is diminished as compared to wild-type
Lactobacillus leichmannii
2.4.2.6
G9T
Ser remains a better substituent for Gly-9 than Thr, kcat is diminished as compared to wild-type
Lactobacillus leichmannii
2.4.2.6
G9W
can not improve 2',3'-dideoxyribose transfer reaction, kcat is diminished as compared to wild-type
Lactobacillus leichmannii
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.2.6
0.25
-
2'-deoxycytidine
wild-type
Lactobacillus leichmannii
2.4.2.6
0.3
-
2'-deoxycytidine
wild-type
Lactobacillus fermentum
2.4.2.6
0.3
-
2'-deoxycytidine
mutant G9S
Lactobacillus leichmannii
2.4.2.6
0.38
-
2',3'-dideoxycytidine
wild-type
Lactobacillus fermentum
2.4.2.6
0.39
-
2'-deoxycytidine
mutant A15T
Lactobacillus fermentum
2.4.2.6
0.8
-
2',3'-dideoxycytidine
mutant A15T
Lactobacillus fermentum
2.4.2.6
0.92
-
2',3'-dideoxycytidine
mutant G9S
Lactobacillus leichmannii
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.4.2.6
18080
-
native enzyme, mass spectrometry
Lactobacillus leichmannii
2.4.2.6
18110
-
mutant, mass spectrometry
Lactobacillus leichmannii
2.4.2.6
18900
-
native enzyme, mass spectrometry
Lactobacillus fermentum
2.4.2.6
18930
-
mutant, mass spectrometry
Lactobacillus fermentum
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.4.2.6
-
Lactobacillus fermentum
2.4.2.6
-
Lactobacillus leichmannii
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.4.2.6
0.01
-
wild-type, with 2',3'-dideoxyguanosine and thymine as substrates; wild-type, with 2',3'-dideoxyinosine and adenine as substrates
Lactobacillus fermentum
2.4.2.6
0.01
-
wild-type, with 2',3'didehydro-2',3'-dideoxythymidine and adenine as substrates; wild-type, with 2',3'-dideoxyguanosine and thymine as substrates; wild-type, with 2',3'-dideoxyinosine and adenine as substrates; wild-type, with 2'-deoxycytidine and hypoxanthine as substrates
Lactobacillus leichmannii
2.4.2.6
0.017
-
wild-type, with 2',3'-dideoxythymidine and adenine as substrates
Lactobacillus leichmannii
2.4.2.6
0.02
-
mutant G9S, with 2'-deoxyinosine and adenine as substrates
Lactobacillus fermentum
2.4.2.6
0.03
-
wild-type, with 2',3'-dideoxythymidine and adenine as substrates
Lactobacillus fermentum
2.4.2.6
0.036
-
wild-type, with 2',3'didehydro-2',3'-dideoxythymidine and adenine as substrates
Lactobacillus fermentum
2.4.2.6
0.04
-
mutant G9S, with 2',3'-dideoxyinosine and adenine as substrates; wild-type, with 2',3'-dideoxycytidine and hypoxanthine as substrates
Lactobacillus leichmannii
2.4.2.6
0.05
-
wild-type, with 2',3'-dideoxycytidine and hypoxanthine as substrates
Lactobacillus fermentum
2.4.2.6
0.056
-
wild-type, with 2',3'-dideoxyuridine and cytosine as substrates
Lactobacillus leichmannii
2.4.2.6
0.06
-
mutant G9S, with 2',3'-dideoxyinosine and adenine as substrates; wild-type, with 2'-deoxycytidine and hypoxanthine as substrates
Lactobacillus fermentum
2.4.2.6
0.19
-
wild-type, with 2',3'-dideoxyuridine and cytosine as substrates
Lactobacillus fermentum
2.4.2.6
0.2
-
mutant G9S, with 2',3'didehydro-2',3'-dideoxythymidine and adenine as substrates
Lactobacillus leichmannii
2.4.2.6
0.25
-
mutant G9S, with 3'-dideoxythymidine and adenine as substrates
Lactobacillus leichmannii
2.4.2.6
0.4
-
mutant G9S, with 2',3'-dideoxyguanosine and thymine as substrates
Lactobacillus leichmannii
2.4.2.6
0.47
-
mutant G9S, with 2'-deoxyinosine and adenine as substrates
Lactobacillus leichmannii
2.4.2.6
0.5
-
mutant G9S, with 3'-dideoxythymidine and adenine as substrates
Lactobacillus fermentum
2.4.2.6
0.57
-
mutant G9S, with 2'-deoxyguanosine and thymine
Lactobacillus fermentum
2.4.2.6
0.6
-
mutant G9S, with 2',3'-dideoxyguanosine and thymine as substrates
Lactobacillus fermentum
2.4.2.6
0.8
-
mutant G9S, with 2',3'-dideoxycytidine and hypoxanthine as substrates
Lactobacillus leichmannii
2.4.2.6
1.04
-
mutant G9S, with 2'-deoxythymidine and adenine as substrates
Lactobacillus leichmannii
2.4.2.6
1.08
-
wild-type, with 2'-deoxyinosine and adenine as substrates
Lactobacillus fermentum
2.4.2.6
1.1
-
mutant G9S, with 2'-deoxyguanosine and thymine
Lactobacillus leichmannii
2.4.2.6
1.25
-
mutant G9S, with 2'-deoxycytidine and hypoxanthine as substrates
Lactobacillus leichmannii
2.4.2.6
1.29
-
mutant G9S, with 2',3'-dideoxycytidine and adenine as substrates
Lactobacillus fermentum
2.4.2.6
1.3
-
mutant G9S, with 2',3'-dideoxyuridine and cytosine as substrates
Lactobacillus leichmannii
2.4.2.6
1.6
-
mutant G9S, with 2',3'-dideoxyuridine and cytosine as substrates
Lactobacillus fermentum
2.4.2.6
1.9
-
mutant G9S, with 2'-deoxythymidine and adenine as substrates
Lactobacillus fermentum
2.4.2.6
3.1
-
mutant G9S, with 2'-deoxyuridine and cytosine as substrates
Lactobacillus leichmannii
2.4.2.6
3.6
-
wild-type, with 2'-deoxyinosine and adenine as substrates
Lactobacillus leichmannii
2.4.2.6
4.95
-
wild-type, with 2'-deoxyguanosine and thymine
Lactobacillus fermentum
2.4.2.6
5.2
-
wild-type, with 2'-deoxyguanosine and thymine
Lactobacillus leichmannii
2.4.2.6
6
-
mutant G9S, with 2',3'-dideoxycytidine and adenine as substrates
Lactobacillus leichmannii
2.4.2.6
6.8
-
mutant G9S, with 2',3'-dideoxycytidine and hypoxanthine as substrates
Lactobacillus fermentum
2.4.2.6
6.9
-
wild-type, with 2',3'-dideoxycytidine and adenine as substrates
Lactobacillus fermentum
2.4.2.6
7.1
-
mutant G9S, with 2'-deoxycytidine and hypoxanthine as substrates
Lactobacillus fermentum
2.4.2.6
9.28
-
mutant G9S, with 2',3'didehydro-2',3'-dideoxythymidine and adenine as substrates
Lactobacillus fermentum
2.4.2.6
9.3
-
mutant G9S, with 2'-deoxycytidine and adenine as substrates
Lactobacillus fermentum
2.4.2.6
11.9
-
wild-type, with 2'-deoxythymidine and adenine as substrates
Lactobacillus leichmannii
2.4.2.6
12.7
-
mutant G9S, with 2'-deoxyuridine and cytosine as substrates
Lactobacillus fermentum
2.4.2.6
20.1
-
wild-type, with 2'-deoxythymidine and adenine as substrates
Lactobacillus fermentum
2.4.2.6
27.1
-
mutant G9S, with 2'-deoxycytidine and adenine as substrates
Lactobacillus leichmannii
2.4.2.6
29
-
wild-type, with 2',3'-dideoxycytidine and adenine as substrates
Lactobacillus leichmannii
2.4.2.6
40.2
-
wild-type, with 2'-deoxyuridine and cytosine as substrates
Lactobacillus fermentum
2.4.2.6
42.5
-
wild-type, with 2'-deoxyuridine and cytosine as substrates
Lactobacillus leichmannii
2.4.2.6
83.7
-
wild-type, with 2'-deoxycytidine and adenine as substrates
Lactobacillus fermentum
2.4.2.6
173
-
wild-type, with 2'-deoxycytidine and adenine as substrates
Lactobacillus leichmannii
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.2.6
2',3'-dideoxycytidine + adenine
-
704429
Lactobacillus fermentum
2',3'-dideoxyadenosine + cytosine
-
-
-
?
2.4.2.6
2',3'-dideoxycytidine + adenine
-
704429
Lactobacillus leichmannii
2',3'-dideoxyadenosine + cytosine
-
-
-
?
2.4.2.6
2',3'-dideoxycytidine + adenine
-
704429
Lactobacillus fermentum PAK9
2',3'-dideoxyadenosine + cytosine
-
-
-
?
2.4.2.6
2',3'-dideoxycytidine + hypoxanthine
-
704429
Lactobacillus fermentum
2',3'-dideoxyinosine + cytosine
-
-
-
?
2.4.2.6
2',3'-dideoxycytidine + hypoxanthine
-
704429
Lactobacillus leichmannii
2',3'-dideoxyinosine + cytosine
-
-
-
?
2.4.2.6
2',3'-dideoxycytidine + hypoxanthine
-
704429
Lactobacillus fermentum PAK9
2',3'-dideoxyinosine + cytosine
-
-
-
?
2.4.2.6
2',3'-dideoxyguanosine + thymine
-
704429
Lactobacillus fermentum
2',3'-dideoxythymidine + guanine
-
-
-
?
2.4.2.6
2',3'-dideoxyguanosine + thymine
-
704429
Lactobacillus leichmannii
2',3'-dideoxythymidine + guanine
-
-
-
?
2.4.2.6
2',3'-dideoxyguanosine + thymine
-
704429
Lactobacillus fermentum PAK9
2',3'-dideoxythymidine + guanine
-
-
-
?
2.4.2.6
2',3'-dideoxyinosine + adenine
-
704429
Lactobacillus fermentum
2',3'-dideoxyadenosine + hypoxanthine
-
-
-
?
2.4.2.6
2',3'-dideoxyinosine + adenine
-
704429
Lactobacillus leichmannii
2',3'-dideoxyadenosine + hypoxanthine
-
-
-
?
2.4.2.6
2',3'-dideoxyinosine + adenine
-
704429
Lactobacillus fermentum PAK9
2',3'-dideoxyadenosine + hypoxanthine
-
-
-
?
2.4.2.6
2',3'-dideoxythymidine + adenine
-
704429
Lactobacillus fermentum
2',3'-dideoxyadenosine + thymine
-
-
-
?
2.4.2.6
2',3'-dideoxythymidine + adenine
-
704429
Lactobacillus leichmannii
2',3'-dideoxyadenosine + thymine
-
-
-
?
2.4.2.6
2',3'-dideoxythymidine + adenine
-
704429
Lactobacillus fermentum PAK9
2',3'-dideoxyadenosine + thymine
-
-
-
?
2.4.2.6
2',3'-dideoxyuridine + cytosine
-
704429
Lactobacillus fermentum
2',3'-dideoxycytidine + uracil
-
-
-
?
2.4.2.6
2',3'-dideoxyuridine + cytosine
-
704429
Lactobacillus leichmannii
2',3'-dideoxycytidine + uracil
-
-
-
?
2.4.2.6
2',3'didehydro-2',3'-dideoxythymidine + adenine
-
704429
Lactobacillus fermentum
2',3'didehydro-2',3'-dideoxyadenosine + thymine
-
-
-
?
2.4.2.6
2',3'didehydro-2',3'-dideoxythymidine + adenine
-
704429
Lactobacillus leichmannii
2',3'didehydro-2',3'-dideoxyadenosine + thymine
-
-
-
?
2.4.2.6
2'-deoxycytidine + adenine
-
704429
Lactobacillus fermentum
2'-deoxyadenosine + cytosine
-
-
-
?
2.4.2.6
2'-deoxycytidine + adenine
-
704429
Lactobacillus leichmannii
2'-deoxyadenosine + cytosine
-
-
-
?
2.4.2.6
2'-deoxycytidine + hypoxanthine
-
704429
Lactobacillus fermentum
2'-deoxyinosine + cytosine
-
-
-
?
2.4.2.6
2'-deoxycytidine + hypoxanthine
-
704429
Lactobacillus leichmannii
2'-deoxyinosine + cytosine
-
-
-
?
2.4.2.6
2'-deoxyguanosine + thymine
-
704429
Lactobacillus fermentum
2'-deoxythymidine + guanine
-
-
-
?
2.4.2.6
2'-deoxyguanosine + thymine
-
704429
Lactobacillus leichmannii
2'-deoxythymidine + guanine
-
-
-
?
2.4.2.6
2'-deoxyinosine + adenine
-
704429
Lactobacillus fermentum
2'-deoxyadenosine + hypoxanthine
-
-
-
?
2.4.2.6
2'-deoxyinosine + adenine
-
704429
Lactobacillus leichmannii
2'-deoxyadenosine + hypoxanthine
-
-
-
?
2.4.2.6
2'-deoxythymidine + adenine
-
704429
Lactobacillus fermentum
2'-deoxyadenosine + thymine
-
-
-
?
2.4.2.6
2'-deoxythymidine + adenine
-
704429
Lactobacillus leichmannii
2'-deoxyadenosine + thymine
-
-
-
?
2.4.2.6
2'-deoxyuridine + cytosine
-
704429
Lactobacillus fermentum
2'-deoxycytidine + uracil
-
-
-
?
2.4.2.6
2'-deoxyuridine + cytosine
-
704429
Lactobacillus leichmannii
2'-deoxycytidine + uracil
-
-
-
?
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.4.2.6
0.047
-
2',3'-dideoxycytidine
wild-type
Lactobacillus fermentum
2.4.2.6
2.54
-
2',3'-dideoxycytidine
mutant G9S
Lactobacillus leichmannii
2.4.2.6
5.36
-
2'-deoxycytidine
mutant A15T
Lactobacillus fermentum
2.4.2.6
10.4
-
2',3'-dideoxycytidine
mutant A15T
Lactobacillus fermentum
2.4.2.6
12.4
-
2'-deoxycytidine
mutant G9S
Lactobacillus leichmannii
2.4.2.6
45.1
-
2'-deoxycytidine
wild-type
Lactobacillus fermentum
2.4.2.6
62.2
-
2'-deoxycytidine
wild-type
Lactobacillus leichmannii
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
2.4.2.6
0.12
-
2',3'-dideoxycytidine
wild-type
Lactobacillus fermentum
2.4.2.6
9.1
-
2',3'-dideoxycytidine
mutant G9S
Lactobacillus leichmannii
2.4.2.6
13
-
2',3'-dideoxycytidine
mutant A15T
Lactobacillus fermentum
2.4.2.6
14
-
2'-deoxycytidine
mutant A15T
Lactobacillus fermentum
2.4.2.6
41
-
2'-deoxycytidine
mutant G9S
Lactobacillus leichmannii
2.4.2.6
150
-
2'-deoxycytidine
wild-type
Lactobacillus fermentum
2.4.2.6
250
-
2'-deoxycytidine
wild-type
Lactobacillus leichmannii
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
2.4.2.6
0.12
-
2',3'-dideoxycytidine
wild-type
Lactobacillus fermentum
2.4.2.6
9.1
-
2',3'-dideoxycytidine
mutant G9S
Lactobacillus leichmannii
2.4.2.6
13
-
2',3'-dideoxycytidine
mutant A15T
Lactobacillus fermentum
2.4.2.6
14
-
2'-deoxycytidine
mutant A15T
Lactobacillus fermentum
2.4.2.6
41
-
2'-deoxycytidine
mutant G9S
Lactobacillus leichmannii
2.4.2.6
150
-
2'-deoxycytidine
wild-type
Lactobacillus fermentum
2.4.2.6
250
-
2'-deoxycytidine
wild-type
Lactobacillus leichmannii