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Literature summary extracted from

  • Harayama, T.; Shindou, H.; Ogasawara, R.; Suwabe, A.; Shimizu, T.
    Identification of a novel noninflammatory biosynthetic pathway of platelet-activating factor (2008), J. Biol. Chem., 283, 11097-11106.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.67 for transfection of CHO-K1 cells Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.67 F174A mutation of hydrophobic residue for measuring the remaining activity using acetyl-CoA and palmitoyl-CoA as acyl donors Mus musculus
2.3.1.67 I160A mutation of hydrophobic residue for measuring the remaining activity using acetyl-CoA and palmitoyl-CoA as acyl donors Mus musculus
2.3.1.67 I162A mutation of hydrophobic residue for measuring the remaining activity using acetyl-CoA and palmitoyl-CoA as acyl donors Mus musculus
2.3.1.67 I167A mutation of hydrophobic residue for measuring the remaining activity using acetyl-CoA and palmitoyl-CoA as acyl donors Mus musculus
2.3.1.67 I170A mutation of hydrophobic residue for measuring the remaining activity using acetyl-CoA and palmitoyl-CoA as acyl donors Mus musculus
2.3.1.67 L166A mutation of hydrophobic residue for measuring the remaining activity using acetyl-CoA and palmitoyl-CoA as acyl donors Mus musculus
2.3.1.67 V173A mutation of hydrophobic residue for measuring the remaining activity using acetyl-CoA and palmitoyl-CoA as acyl donors Mus musculus
2.3.1.67 V175A mutation of hydrophobic residue for measuring the remaining activity using acetyl-CoA and palmitoyl-CoA as acyl donors Mus musculus
2.3.1.67 W163A mutation of hydrophobic residue for measuring the remaining activity using acetyl-CoA and palmitoyl-CoA as acyl donors Mus musculus
2.3.1.67 Y169A mutation of hydrophobic residue for measuring the remaining activity using acetyl-CoA and palmitoyl-CoA as acyl donors Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.67 Cu2+
-
 Mus musculus
2.3.1.67 Fe2+
-
 Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.67 0.0824
-
 acetyl-CoA apparent KM Mus musculus
2.3.1.67 0.1282
-
 1-O-hexadecyl-sn-glycero-3-phosphocholine apparent KM Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.67 microsome
-
 Mus musculus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.67 60000
-
 determined by SDS-PAGE and Western Blot analysis Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.67 1-O-hexadecyl-sn-glycero-3-phosphocholine + acetyl-CoA Mus musculus lyso-PAF, lyso-platelet-activating factor 1-O-hexadecyl-2-acetyl-sn-glycero-3-phosphocholine + CoA PAF, platelet-activating factor ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.67 Mus musculus Q3TFD2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.67 LPCAT1 is partially purified from microsomes from mouse tissue using a DEAE-Sepharose column Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.67 brain
-
 Mus musculus
-
2.3.1.67 lung
-
 Mus musculus
-
2.3.1.67 spleen
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.67 0.000017
-
 brain, Ca2+ independent lyso-PAF AT activity in the presence of EDTA, in the presence of Ca2+ 0.000104 Mus musculus
2.3.1.67 0.000104
-
 spleen, Ca2+ independent lyso-PAF AT activity in the presence of EDTA, in the presence of Ca2+ 0.000470 Mus musculus
2.3.1.67 0.00033
-
 lung, Ca2+ independent lyso-PAF AT activity in the presence of EDTA, in the presence of Ca2+ 0.000452 Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.67 1-O-hexadecyl-sn-glycero-3-phosphocholine + acetyl-CoA lyso-PAF, lyso-platelet-activating factor Mus musculus 1-O-hexadecyl-2-acetyl-sn-glycero-3-phosphocholine + CoA PAF, platelet-activating factor ?
2.3.1.67 additional information LPCAT1 recognizes a wide range of acyl-CoAs as substrates, ranging from acetyl-CoA to palmitoyl-CoA Mus musculus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.67 LPCAT1 Ca2+ independent lyso-PAF acetyltransferase Mus musculus
2.3.1.67 lyso-PAF acetyltransferase
-
 Mus musculus
2.3.1.67 lyso-PAF AT
-
 Mus musculus
2.3.1.67 lysophosphatidylcholine acyltransferase 1
-
 Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.67 7.4
-
 activity assay Mus musculus

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.67 acetyl-CoA
-
 Mus musculus