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Literature summary extracted from

  • Hegde, S.S.; Shrader, T.E.
    FemABX family members are novel nonribosomal peptidyltransferases and important pathogen-specific drug targets (2001), J. Biol. Chem., 276, 6998-7003.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.2.16
-
 Weissella viridescens

Protein Variants

EC Number Protein Variants Comment Organism
2.3.2.16 F196L 26% of wild-type activity Weissella viridescens
2.3.2.16 F196Y 87% of wild-type activity Weissella viridescens
2.3.2.16 F305L 16% of wild-type activity Weissella viridescens
2.3.2.16 F305Y 98% of wild-type activity Weissella viridescens
2.3.2.16 G292V inactive Weissella viridescens
2.3.2.16 G319S 13% of wild-type activity Weissella viridescens
2.3.2.16 G319V 5% of wild-type activity Weissella viridescens
2.3.2.16 K306A 8% of wild-type activity Weissella viridescens
2.3.2.16 K306C 38% of wild-type activity Weissella viridescens
2.3.2.16 K306N 9% of wild-type activity Weissella viridescens
2.3.2.16 K306R 25% of wild-type activity Weissella viridescens
2.3.2.16 P110F inactive Weissella viridescens
2.3.2.16 P110H inactive Weissella viridescens
2.3.2.16 Q144E 11% of wild-type activity Weissella viridescens
2.3.2.16 Q144T 79% of wild-type activity Weissella viridescens
2.3.2.16 Q29E inactive Weissella viridescens
2.3.2.16 Q29T inactive Weissella viridescens
2.3.2.16 Y216F 41% of wild-type activity Weissella viridescens
2.3.2.16 Y216L inactive Weissella viridescens
2.3.2.16 Y73F 40% of wild-type activity Weissella viridescens
2.3.2.16 Y73L 40% of wild-type activity Weissella viridescens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.2.16 39360
-
 1 * 39360, calculated Weissella viridescens
2.3.2.16 40000
-
 gel filtration Weissella viridescens

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.16 Weissella viridescens Q9EY50
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.16
-
 Weissella viridescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.16 glycyl-tRNA(E.coli) + UDP-MurNAc-Ala-D-Glu-Lys-D-Ala-D-Ala addition of serine and alanine is preferred over glycine Weissella viridescens tRNA(E.coli) + UDP-MurNAc-Ala-D-Glu-(N6-Gly)Lys-D-Ala-D-Ala
-
 ?
2.3.2.16 L-alanyl-tRNA(E.coli) + UDP-MurNAc-Ala-D-Glu-Lys-D-Ala-D-Ala addition of serine and alanine is preferred over glycine Weissella viridescens tRNA(E.coli) + UDP-MurNAc-Ala-D-Glu-(N6-L-Ala)Lys-D-Ala-D-Ala
-
 ?
2.3.2.16 L-seryl-tRNA(E.coli) + UDP-MurNAc-Ala-D-Glu-Lys-D-Ala-D-Ala addition of serine and alanine is preferred over glycine Weissella viridescens tRNA(E.coli) + UDP-MurNAc-Ala-D-Glu-(N6-L-Ser)Lys-D-Ala-D-Ala
-
 ?
2.3.2.16 additional information Assays with mixtures of alanyl-, seryl-, and glycyl-tRNAs result in mixtures of the expected UDP-MurNAc hexapeptides, but no larger species. FemX adds only the first residue of the interchain peptide Weissella viridescens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.2.16 monomer 1 * 39360, calculated Weissella viridescens

Synonyms

EC Number Synonyms Comment Organism
2.3.2.16 FemX
-
 Weissella viridescens