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Literature summary extracted from

  • Abdian, P.L.; Lellouch, A.C.; Gautier, C.; Ielpi, L.; Geremia, R.A.
    Identification of essential amino acids in the bacterial (2000), J. Biol. Chem., 275, 40568-40575.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.252 recombinant mutant AceA expressed in Escherichia coli and in Xanthomonas campestris gumH- strain Komagataeibacter xylinus

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.252 E287A mutant enzyme loses activity in vivo and in vitro Komagataeibacter xylinus
2.4.1.252 E295A mutant enzyme retains residual activity in the in vivo assay Komagataeibacter xylinus
2.4.1.252 H127A retains reduced but significant activity both in vitro and in vivo Komagataeibacter xylinus
2.4.1.252 K211A mutant enzyme loses activity in vivo and in vitro Komagataeibacter xylinus
2.4.1.252 S162A retains reduced but significant activity both in vitro and in vivo Komagataeibacter xylinus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.252 membrane associated with Komagataeibacter xylinus 16020
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Organism

EC Number Organism UniProt Comment Textmining
2.4.1.252 Komagataeibacter xylinus
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-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.252 GDP-mannose + D-Glc-beta-(1->4)-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol AceA is not able to use free cellobiose as a substrate, indicating that the pyrophosphate-lipid moiety is needed for enzymatic activity Komagataeibacter xylinus GDP + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol characterisation of the in vitro reaction product ?
2.4.1.252 additional information AceA Lys211 and Glu295 are involved in nucleotide sugar donor binding. Glu287 of the EX7E is a potential catalytic residue Komagataeibacter xylinus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.252 alpha-mannosyltransferase AceA
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 Komagataeibacter xylinus