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Characterization of three potato lipoxygenases with distinct enzymatic activities and different organ-specific and wound-regulated expression patterns

Royo, J.; Vancanneyt, G.; Perez, A.G.; Sanz, C.; Störmann, K.; Rosahl, S.; Sanchez-Serrano, J.J.; J. Biol. Chem. 271, 21012-21009 (1996)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.13.11.12
expression in Escherichia coli
Solanum tuberosum
1.13.11.58
expression in Escherichia coli
Solanum tuberosum
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.13.11.12
Solanum tuberosum
-
L. cv Desiree
-
1.13.11.58
Solanum tuberosum
P37831
L. cv Desiree
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.13.11.12
flower bud
Lox2 and Lox3 are present to a lesser extent, compared to leaf
Solanum tuberosum
-
1.13.11.12
leaf
LOX2 level starts to rise steadily up to 24 h after jasmonic acid treatment, maximal lox3 levels are attained around 30 min after wounding or jasmonic acid treatment
Solanum tuberosum
-
1.13.11.12
root
Lox3 is detected in roots, Lox2 levels in roots is below limit of detection
Solanum tuberosum
-
1.13.11.58
bud
Lox1 transcripts are predominantly detected in tubers and roots and to a much lesser extent in buds and leaves
Solanum tuberosum
-
1.13.11.58
leaf
Lox1 transcripts are predominantly detected in tubers and roots and to a much lesser extent in buds and leaves
Solanum tuberosum
-
1.13.11.58
root
Lox1 transcripts are predominantly detected in tubers and roots and to a much lesser extent in buds and leaves
Solanum tuberosum
-
1.13.11.58
tuber
Lox1 transcripts are predominantly detected in tubers and roots and to a much lesser extent in buds and leaves. along tuber formation, Lox1 class transcripts are detected at the stolon stage, and their steady state levels increases during the early stages of tuber development
Solanum tuberosum
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.13.11.12
alpha-linolenate + O2
Lox2 and Lox3 are more active against linolenate compared to linoleate
704382
Solanum tuberosum
(9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoate
-
-
-
?
1.13.11.12
linoleate + O2
Lox2 and Lox3 are more active against linolenate compared to linoleate
704382
Solanum tuberosum
(9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoate
the R/S stereoconfiguration of the product is not determined
-
-
?
1.13.11.58
alpha-linolenate + O2
-
704382
Solanum tuberosum
(10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoate
96% (10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoate and 2.1% (9Z,11E,15Z)-13-hydroperoxy-9,11,15-octadecatrienoate
-
-
?
1.13.11.58
linoleate + O2
linoleate (abundant in membrane lipids of tubers) is preferred to linolenate as substrate
704382
Solanum tuberosum
(10E,12Z)-9-hydroperoxy-10,12-octadecadienoate
the R/S stereoconfiguration of the product is not determined
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.13.11.12
6
-
-
Solanum tuberosum
1.13.11.58
6
-
-
Solanum tuberosum
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.13.11.12
expression in Escherichia coli
Solanum tuberosum
1.13.11.58
expression in Escherichia coli
Solanum tuberosum
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.13.11.12
flower bud
Lox2 and Lox3 are present to a lesser extent, compared to leaf
Solanum tuberosum
-
1.13.11.12
leaf
LOX2 level starts to rise steadily up to 24 h after jasmonic acid treatment, maximal lox3 levels are attained around 30 min after wounding or jasmonic acid treatment
Solanum tuberosum
-
1.13.11.12
root
Lox3 is detected in roots, Lox2 levels in roots is below limit of detection
Solanum tuberosum
-
1.13.11.58
bud
Lox1 transcripts are predominantly detected in tubers and roots and to a much lesser extent in buds and leaves
Solanum tuberosum
-
1.13.11.58
leaf
Lox1 transcripts are predominantly detected in tubers and roots and to a much lesser extent in buds and leaves
Solanum tuberosum
-
1.13.11.58
root
Lox1 transcripts are predominantly detected in tubers and roots and to a much lesser extent in buds and leaves
Solanum tuberosum
-
1.13.11.58
tuber
Lox1 transcripts are predominantly detected in tubers and roots and to a much lesser extent in buds and leaves. along tuber formation, Lox1 class transcripts are detected at the stolon stage, and their steady state levels increases during the early stages of tuber development
Solanum tuberosum
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.13.11.12
alpha-linolenate + O2
Lox2 and Lox3 are more active against linolenate compared to linoleate
704382
Solanum tuberosum
(9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoate
-
-
-
?
1.13.11.12
linoleate + O2
Lox2 and Lox3 are more active against linolenate compared to linoleate
704382
Solanum tuberosum
(9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoate
the R/S stereoconfiguration of the product is not determined
-
-
?
1.13.11.58
alpha-linolenate + O2
-
704382
Solanum tuberosum
(10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoate
96% (10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoate and 2.1% (9Z,11E,15Z)-13-hydroperoxy-9,11,15-octadecatrienoate
-
-
?
1.13.11.58
linoleate + O2
linoleate (abundant in membrane lipids of tubers) is preferred to linolenate as substrate
704382
Solanum tuberosum
(10E,12Z)-9-hydroperoxy-10,12-octadecadienoate
the R/S stereoconfiguration of the product is not determined
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.13.11.12
6
-
-
Solanum tuberosum
1.13.11.58
6
-
-
Solanum tuberosum