Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Savage, T.J.; Hatch, M.W.; Croteau, R.
    Monoterpene synthases of Pinus contorta and related conifers. A new class of terpenoid cyclase (1994), J. Biol. Chem., 269, 4012-4020.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.3.52 diethyl dicarbonate lack of substrate protection against inactivation Pinus contorta
4.2.3.107 diethyl dicarbonate formation of a substrate-metal ion complex does not protect against inhibition Pinus contorta

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.52 0.0078
-
 geranyl diphosphate 32°C Pinus contorta

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.3.107 soluble
-
 Pinus contorta
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.52 Cs+ activates Pinus contorta
4.2.3.52 Fe2+ the enzyme requires either Mn2+ or Fe2+ as divalent metal ion cofactor Pinus contorta
4.2.3.52 K+ activates Pinus contorta
4.2.3.52 Mn2+ the enzyme requires either Mn2+ or Fe2+ as divalent metal ion cofactor, maximal activity at 1 mM MnCl2 Pinus contorta
4.2.3.52 additional information no activation by Mg2+, Li+ or Na+ Pinus contorta
4.2.3.52 NH4+ activates Pinus contorta
4.2.3.52 Rb+ activates Pinus contorta
4.2.3.107 Cs+ about 50% of the activation by K+ Pinus contorta
4.2.3.107 Fe2+ or Mn2+, required Pinus contorta
4.2.3.107 K+ and Rb+, best activation Pinus contorta
4.2.3.107 Mn2+ or Fe2+, required Pinus contorta
4.2.3.107 NH4+ about 80% of the activation by K+ Pinus contorta
4.2.3.107 Rb+ and K+, activation Pinus contorta

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.3.52 67000
-
 gel filtration Pinus contorta

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.52 Pinus contorta
-
-
-
4.2.3.107 Pinus contorta
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
4.2.3.52 the enzyme is extremely sensitive to thiol oxidation. Cyclase activity is completely lost when the partially purified xylem preparation is dialyzed against buffer (pH 7.8) devoid of thiol-protecting reagents (dithiothreitol or 2-mercaptoethanol). Other reducing agents, such as sodium ascorbate (5 mM) or sodium metabisulfite (5 mM), fail to protect the cyclases from inactivation, whereas readdition of 5 mM dithiothreitol to the dialyzed preparations restores 54% of the original activity Pinus contorta

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.3.52
-
 Pinus contorta
4.2.3.107 partial Pinus contorta

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.3.52 xylem of 2-year-old Pinus contorta Pinus contorta
-
4.2.3.107 phloem 29% of xylem activity Pinus contorta
-
4.2.3.107 root 22% of xylem activity Pinus contorta
-
4.2.3.107 xylem highest enzymic activity Pinus contorta
-

Storage Stability

EC Number Storage Stability Organism
4.2.3.52 0-4°C, partially purified preparation is very stable Pinus contorta

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.52 geranyl diphosphate
-
 Pinus contorta (4S)-beta-phellandrene + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.3.52 monomer 1 * 67000, SDS-PAGE Pinus contorta

Synonyms

EC Number Synonyms Comment Organism
4.2.3.52 phellandrene synthase
-
 Pinus contorta

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.3.52 7.8
-
-
 Pinus contorta
4.2.3.107 7.8
-
-
 Pinus contorta

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.3.52 6.6 8.5 50% of maximal activity at pH 6.6 and at pH 8.5 Pinus contorta

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.2.3.52 Pinus contorta isoelectric focusing
-
 4.75