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Literature summary extracted from
- Purification and characterization of a flavoprotein from bovine thyroid with iodotyrosine deiodinase activity (1979), J. Biol. Chem., 254, 12318-12325.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.21.1.1 | 2-mercaptoethanol | maximal activity at 50 mM | Bos taurus |  |
| 1.21.1.1 | FAD | up to 1.5fold stimulation | Bos taurus | |
| 1.21.1.1 | FMN | up to 1.5fold stimulation | Bos taurus | |
| 1.21.1.1 | additional information | enzyme activity increases at high ionic strength | Bos taurus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.21.1.1 | 0.0025 | - |
3,5-diiodo-L-tyrosine | pH 7.4, 38°C | Bos taurus | |
| 1.21.1.1 | 0.05 | - |
3-iodo-L-tyrosine | pH 7.4, 38°C | Bos taurus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.21.1.1 | microsome | - |
Bos taurus | - |
- |
| 1.21.1.1 | mitochondrion | - |
Bos taurus | 5739 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.21.1.1 | 22300 | - |
2 * 22300, SDS-PAGE | Bos taurus |
| 1.21.1.1 | 42000 | - |
gel filtration | Bos taurus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.21.1.1 | Bos taurus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.21.1.1 | - |
Bos taurus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.21.1.1 | thyroid gland | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.21.1.1 | 7800 | - |
pH 7.4, 38°C | Bos taurus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.21.1.1 | -15 to -20°C in 10 mM phosphate buffer, stable for months | Bos taurus |
| 1.21.1.1 | 0 to 4°C in 10 mM phosphate buffer, stable for weeks | Bos taurus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.21.1.1 | 3,5-diiodo-L-tyrosine + NADPH + H+ | - |
Bos taurus | 3-iodo-L-tyrosine + NADP+ + I- | - |
? | |
| 1.21.1.1 | 3-iodo-L-tyrosine + NADPH + H+ | - |
Bos taurus | L-tyrosine + NADP+ + I- | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.21.1.1 | dimer | 2 * 22300, SDS-PAGE | Bos taurus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.21.1.1 | 10 | - |
3,5-diiodo-L-tyrosine | pH 7.4, 38°C | Bos taurus | |
| 1.21.1.1 | 18 | - |
3-iodo-L-tyrosine | pH 7.4, 38°C | Bos taurus | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.21.1.1 | 6.3 | - |
- |
Bos taurus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.21.1.1 | FMN | 1 mol of FMN per mol of protein | Bos taurus | |
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