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Literature summary extracted from

  • Kurosawa, N.; Hirata, T.; Suzuki, H.
    Characterization of putative tryptophan monooxygenase from Ralstonia solanacearum (2009), J. Biochem., 146, 23-32.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.16.4 additional information pronase is most effective for activation. Trypsin, chymotrypsin, V8 protease and lysylendoprotease also activate PTMO, but the activity obtained using these proteases is only 5% of that with pronase Ralstonia solanacearum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.16.4 pBR322 containing the open reading frame of PTMO, PCR products inserted into the EcoRV site of pBluescript vector, expressed from vector pET 22b(+) in Escherichia coli BL21(DE3) Ralstonia solanacearum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.16.4 162000
-
 gel filtration Ralstonia solanacearum

Organism

EC Number Organism UniProt Comment Textmining
1.14.16.4 Ralstonia solanacearum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.16.4 on Ni column Ralstonia solanacearum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.16.4 L-methionine + ? + O2 relative high activity with pronase-treated PTMO Ralstonia solanacearum ?
-
 ?
1.14.16.4 L-phenylalanine + tetrahydropteridine + O2 purified PTMO shows no activity, relative high activity with pronase-treated PTMO Ralstonia solanacearum L-tyrosine + dihydropteridine + H2O
-
 ?
1.14.16.4 L-tryptophan + tetrahydrobiopterin + O2 relative high activity with pronase-treated PTMO Ralstonia solanacearum 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin
-
 ?
1.14.16.4 L-tyrosine + tetrahydropteridine + O2 relative high activity with pronase-treated PTMO Ralstonia solanacearum ?
-
 ?
1.14.16.4 additional information pronase-treated PTMO shows less than 5% activity of that of L-phenylalanine for L-lysine, L-histidine and L-threonine Ralstonia solanacearum ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.16.4 PTMO
-
 Ralstonia solanacearum
1.14.16.4 putative tryptophan monooxygenase
-
 Ralstonia solanacearum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.16.4 7
-
-
 Ralstonia solanacearum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.14.16.4 6.5 7.5
-
 Ralstonia solanacearum

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.16.4 tetrahydrobiopterin
-
 Ralstonia solanacearum