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Literature summary extracted from

  • Tsuchiya, W.; Hasegawa, T.
    Molecular recognition of tryptophan tRNA by tryptophanyl-tRNA synthetase from Aeropyrum pernix K1 (2009), J. Biochem., 145, 635-641.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.2 the trpS gene is cloned into the pET30a+ vector for expression in Escherichia coli BL21-CodonPlus DE3-RIL cells Aeropyrum pernix K1

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.2 0.0012
-
 tRNATrp
-
 Aeropyrum pernix K1
6.1.1.2 0.0017
-
 tRNATrp(A73C) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
6.1.1.2 0.0019
-
 tRNATrp(G2C-G71G) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
6.1.1.2 0.0021
-
 tRNAArg pH 7.5, 60°C Aeropyrum pernix K1
6.1.1.2 0.0051
-
 tRNATrp(A73U) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
6.1.1.2 0.0053
-
 tRNATrp(A36G) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
6.1.1.2 0.0055
-
 tRNATrp(G1C-G72G) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
6.1.1.2 0.0058
-
 tRNATrp(G2U-C71A) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
6.1.1.2 0.0058
-
 tRNATrp(A73G) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
6.1.1.2 0.0058
-
 tRNATrp(A36U) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
6.1.1.2 0.0059
-
 tRNATrp(A36C) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
6.1.1.2 0.006
-
 tRNATrp(HOG1) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
6.1.1.2 0.0098
-
 tRNATrp(A36U, A73G) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
6.1.1.2 0.012
-
 tRNATrp(pppG1) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
6.1.1.2 0.014
-
 tRNATrp(C35A) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
6.1.1.2 0.015
-
 tRNATrp(G2A-C71U) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
6.1.1.2 0.021
-
 tRNATrp(G1U-C72A) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
6.1.1.2 0.021
-
 tRNATrp(C35U) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
6.1.1.2 0.025
-
 tRNATrp(G1A-C72U) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
6.1.1.2 0.038
-
 tRNATrp(C34G) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
6.1.1.2 0.038
-
 tRNATrp(C35G) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
6.1.1.2 0.048
-
 tRNATrp(C34U) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
6.1.1.2 0.06
-
 tRNATrp(C34A) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.2 ATP + L-tryptophan + tRNATrp Aeropyrum pernix K1
-
 AMP + diphosphate + L-tryptophyl-tRNATrp
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.2 Aeropyrum pernix K1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.2 by affinity chromatography using a Cibacron Blue 3GA column Aeropyrum pernix K1

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.2 ATP + L-tryptophan + tRNAArg
-
 Aeropyrum pernix K1 AMP + diphosphate + L-tryptophyl-tRNAArg
-
 ?
6.1.1.2 ATP + L-tryptophan + tRNATrp
-
 Aeropyrum pernix K1 AMP + diphosphate + L-tryptophyl-tRNATrp
-
 ?
6.1.1.2 ATP + L-tryptophan + tRNATrp(A36C) substrate mutant Aeropyrum pernix K1 AMP + diphosphate + L-tryptophyl-tRNATrp(A36C)
-
 ?
6.1.1.2 ATP + L-tryptophan + tRNATrp(A36G) substrate mutant Aeropyrum pernix K1 AMP + diphosphate + L-tryptophyl-tRNATrp(A36G)
-
 ?
6.1.1.2 ATP + L-tryptophan + tRNATrp(A36U) substrate mutant Aeropyrum pernix K1 AMP + diphosphate + L-tryptophyl-tRNATrp(A36U)
-
 ?
6.1.1.2 ATP + L-tryptophan + tRNATrp(A36U, A73G) substrate mutant Aeropyrum pernix K1 AMP + diphosphate + L-tryptophyl-tRNATrp(A36U, A73G)
-
 ?
6.1.1.2 ATP + L-tryptophan + tRNATrp(A73C) substrate mutant Aeropyrum pernix K1 AMP + diphosphate + L-tryptophyl-tRNATrp(A73C)
-
 ?
6.1.1.2 ATP + L-tryptophan + tRNATrp(A73G) substrate mutant Aeropyrum pernix K1 AMP + diphosphate + L-tryptophyl-tRNATrp(A73G)
-
 ?
6.1.1.2 ATP + L-tryptophan + tRNATrp(A73U) substrate mutant Aeropyrum pernix K1 AMP + diphosphate + L-tryptophyl-tRNATrp(A73U)
-
 ?
6.1.1.2 ATP + L-tryptophan + tRNATrp(C34A) substrate mutant Aeropyrum pernix K1 AMP + diphosphate + L-tryptophyl-tRNATrp(C34A)
-
 ?
6.1.1.2 ATP + L-tryptophan + tRNATrp(C34G) substrate mutant Aeropyrum pernix K1 AMP + diphosphate + L-tryptophyl-tRNATrp(C34G)
-
 ?
6.1.1.2 ATP + L-tryptophan + tRNATrp(C34U) substrate mutant Aeropyrum pernix K1 AMP + diphosphate + L-tryptophyl-tRNATrp(C34U)
-
 ?
6.1.1.2 ATP + L-tryptophan + tRNATrp(C35A) substrate mutant Aeropyrum pernix K1 AMP + diphosphate + L-tryptophyl-tRNATrp(C35A)
-
 ?
6.1.1.2 ATP + L-tryptophan + tRNATrp(C35G) substrate mutant Aeropyrum pernix K1 AMP + diphosphate + L-tryptophyl-tRNATrp(C35G)
-
 ?
6.1.1.2 ATP + L-tryptophan + tRNATrp(C35U) substrate mutant Aeropyrum pernix K1 AMP + diphosphate + L-tryptophyl-tRNATrp(C35U)
-
 ?
6.1.1.2 ATP + L-tryptophan + tRNATrp(G1A-C72U) substrate mutant Aeropyrum pernix K1 AMP + diphosphate + L-tryptophyl-tRNATrp(G1A-C72U)
-
 ?
6.1.1.2 ATP + L-tryptophan + tRNATrp(G1C-G72G) substrate mutant Aeropyrum pernix K1 AMP + diphosphate + L-tryptophyl-tRNATrp(G1C-G72G)
-
 ?
6.1.1.2 ATP + L-tryptophan + tRNATrp(G1U-C72A) substrate mutant Aeropyrum pernix K1 AMP + diphosphate + L-tryptophyl-tRNATrp(G1U-C72A)
-
 ?
6.1.1.2 ATP + L-tryptophan + tRNATrp(G2A-C71U) substrate mutant Aeropyrum pernix K1 AMP + diphosphate + L-tryptophyl-tRNATrp(G2A-C71U)
-
 ?
6.1.1.2 ATP + L-tryptophan + tRNATrp(G2C-G71G) substrate mutant Aeropyrum pernix K1 AMP + diphosphate + L-tryptophyl-tRNATrp(G2C-G71G)
-
 ?
6.1.1.2 ATP + L-tryptophan + tRNATrp(G2U-C71A) substrate mutant Aeropyrum pernix K1 AMP + diphosphate + L-tryptophyl-tRNATrp(G2U-C71A)
-
 ?
6.1.1.2 ATP + L-tryptophan + tRNATrp(HOG1) substrate mutant Aeropyrum pernix K1 AMP + diphosphate + L-tryptophyl-tRNATrp(HOG1)
-
 ?
6.1.1.2 ATP + L-tryptophan + tRNATrp(pppG1) substrate mutant Aeropyrum pernix K1 AMP + diphosphate + L-tryptophyl-tRNATrp(pppG1)
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.2 TrpRS
-
 Aeropyrum pernix K1
6.1.1.2 Tryptophanyl-tRNA synthetase
-
 Aeropyrum pernix K1

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.1.1.2 60
-
 tryptophanylation assay Aeropyrum pernix K1

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.2 0.079
-
 tRNATrp(C35G) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
6.1.1.2 0.11
-
 tRNATrp(C34U) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
6.1.1.2 0.13
-
 tRNATrp(C34G) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
6.1.1.2 0.15
-
 tRNATrp(C35A) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
6.1.1.2 0.16
-
 tRNAArg pH 7.5, 60°C Aeropyrum pernix K1
6.1.1.2 0.17
-
 tRNATrp(C34A) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
6.1.1.2 0.27
-
 tRNATrp(A36U, A73G) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
6.1.1.2 0.34
-
 tRNATrp(G1C-G72G) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
6.1.1.2 0.46
-
 tRNATrp(G1U-C72A) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
6.1.1.2 0.47
-
 tRNATrp(C35U) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
6.1.1.2 0.62
-
 tRNATrp(A73G) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
6.1.1.2 1.7
-
 tRNATrp(G2C-G71G) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
6.1.1.2 2.3
-
 tRNATrp(G1A-C72U) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
6.1.1.2 3.2
-
 tRNATrp(HOG1) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
6.1.1.2 4.4
-
 tRNATrp(A36G) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
6.1.1.2 4.5
-
 tRNATrp(pppG1) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
6.1.1.2 5.5
-
 tRNATrp(A73C) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
6.1.1.2 6.1
-
 tRNATrp(A73U) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
6.1.1.2 7.2
-
 tRNATrp(G2U-C71A) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
6.1.1.2 11
-
 tRNATrp(A36C) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
6.1.1.2 16
-
 tRNATrp pH 7.5, 60°C Aeropyrum pernix K1
6.1.1.2 19
-
 tRNATrp(G2A-C71U) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1
6.1.1.2 21
-
 tRNATrp(A36U) substrate mutant, pH 7.5, 60°C Aeropyrum pernix K1

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.2 7.5
-
 tryptophanylation assay Aeropyrum pernix K1

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.2 ATP
-
 Aeropyrum pernix K1