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Literature summary extracted from

  • Kabututu, Z.; Manin, M.; Pointud, J.C.; Maruyama, T.; Nagata, N.; Lambert, S.; Lefrancois-Martinez, A.M.; Martinez, A.; Urade, Y.
    Prostaglandin F2alpha synthase activities of aldo-keto reductase 1B1, 1B3 and 1B7 (2009), J. Biochem., 145, 161-168.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.188 into vector pET-28a and expressed in Escherichia coli BL21 (DE3) Mus musculus
1.1.1.188 into vector pET-28a and expressed in Escherichia coli BL21 (DE3) Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.188 additional information heat treatment of AKR1B1 at 100°C for 5 min completely inactivates the prostaglandin F2alpha synthase activity, which is strictly dependent on the presence of NADPH Homo sapiens
1.1.1.188 additional information heat treatment of AKR1B3 and AKR1B7 at 100°C for 5 min completely inactivates the prostaglandin F2alpha synthase activities, which is strictly dependent on the presence of NADPH Mus musculus
1.1.1.188 sorbinil
-
 Homo sapiens
1.1.1.188 sorbinil significantly inhibits the prostaglandin F2alpha synthase activity of AKR1B3 in a non-competitive fashion, whereas AKR1B7 is not sensitive to the inhibitor Mus musculus
1.1.1.188 Tolrestat
-
 Homo sapiens
1.1.1.188 Tolrestat significantly inhibits the prostaglandin F2alpha synthase activity of AKR1B3 in a non-competitive fashion, whereas AKR1B7 is not sensitive to the inhibitor Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.188 0.0019
-
 prostaglandin H2
-
 Homo sapiens
1.1.1.188 0.0038
-
 prostaglandin H2 AKR1B7 Mus musculus
1.1.1.188 0.0057
-
 NADPH
-
 Homo sapiens
1.1.1.188 0.0074
-
 NADPH AKR1B3 Mus musculus
1.1.1.188 0.0092
-
 NADPH AKR1B7 Mus musculus
1.1.1.188 0.0093
-
 prostaglandin H2 AKR1B3 Mus musculus
1.1.1.188 0.018
-
 prostaglandin H2
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.188 35000
-
 x * 35000, SDS-PAGE Mus musculus
1.1.1.188 35000
-
 x * 35000, SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.188 Homo sapiens P15121
-
-
1.1.1.188 Homo sapiens P42330
-
-
1.1.1.188 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.188 to apparent homogeneity, on Ni-NTA resin and by gel filtration Mus musculus
1.1.1.188 to apparent homogeneity, on Ni-NTA resin and by gel filtration Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.188 kidney AKR1B3 Mus musculus
-
1.1.1.188 lung
-
 Homo sapiens
-
1.1.1.188 placenta
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.188 prostaglandin H2 + NADPH + H+ aldo-keto reductase has prostaglandin F2alpha synthase activity. AKR1B1 is a more efficient prostaglandin F2alpha synthase than AKR1C3 Homo sapiens (5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate + NADP+
-
 ?
1.1.1.188 prostaglandin H2 + NADPH + H+ aldo-keto reductases AKR1B3 and AKR1B7 have prostaglandin F2alpha synthase activity Mus musculus (5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.188 ? x * 35000, SDS-PAGE Mus musculus
1.1.1.188 ? x * 35000, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
1.1.1.188 AKR1B1
-
 Homo sapiens
1.1.1.188 AKR1B3
-
 Mus musculus
1.1.1.188 AKR1B7
-
 Mus musculus
1.1.1.188 AKR1C3
-
 Homo sapiens
1.1.1.188 aldo-keto reductase
-
 Mus musculus
1.1.1.188 aldo-keto reductase
-
 Homo sapiens
1.1.1.188 PGFS
-
 Mus musculus
1.1.1.188 PGFS
-
 Homo sapiens
1.1.1.188 prostaglandin F2alpha synthase
-
 Mus musculus
1.1.1.188 prostaglandin F2alpha synthase
-
 Homo sapiens
1.1.1.188 vas deferens protein
-
 Mus musculus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.188 NADPH
-
 Mus musculus
1.1.1.188 NADPH
-
 Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.188 0.00026
-
 Tolrestat AKR1B3 Mus musculus
1.1.1.188 0.00089
-
 sorbinil AKR1B3 Mus musculus
1.1.1.188 0.0036
-
 Tolrestat
-
 Homo sapiens
1.1.1.188 0.0217
-
 sorbinil
-
 Homo sapiens