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Literature summary extracted from

  • Takagi, M.; Tamaki, H.; Miyamoto, Y.; Leonardi, R.; Hanada, S.; Jackowski, S.; Chohnan, S.
    Pantothenate kinase from the thermoacidophilic archaeon Picrophilus torridus (2010), J. Bacteriol., 192, 233-241.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.33 expressed in Escherichia coli Picrophilus torridus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.33 0.099
-
 ATP
-
 Picrophilus torridus
2.7.1.33 0.621
-
 pantothenate
-
 Picrophilus torridus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.33 Mg2+ activity with 1 mM (plus 2.5 mM ATP): 252 nmol/min/mg Picrophilus torridus
2.7.1.33 Mn2+ addition (1 mM) instead of Mg2+ accelerates activity 1.75fold Picrophilus torridus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.33 33000
-
 x * 33000 Da, SDS-PAGE Picrophilus torridus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.33 Picrophilus torridus Q6L2I5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.33
-
 Picrophilus torridus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.33 0.36
-
 purified recombinant protein Picrophilus torridus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.33 pantothenate + ATP relative activity with GTP: 81%, CTP: 68%, and UTP: 91.8% Picrophilus torridus phosphopantothenate + ADP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.33 ? x * 33000 Da, SDS-PAGE Picrophilus torridus

Synonyms

EC Number Synonyms Comment Organism
2.7.1.33 pantothenate kinase
-
 Picrophilus torridus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.33 55
-
-
 Picrophilus torridus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.1.33 50 70 more than 80% of the maximum activity Picrophilus torridus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.33 0.718
-
 ATP
-
 Picrophilus torridus
2.7.1.33 0.818
-
 pantothenate
-
 Picrophilus torridus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.33 5
-
-
 Picrophilus torridus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.33 4.5 6 more than 70% of the maximum activity Picrophilus torridus