BRENDA - Enzyme Database

Deletion of citrate synthase restores growth of Sinorhizobium meliloti 1021 aconitase mutants

Koziol, U.; Hannibal, L.; Rodriguez, M.C.; Fabiano, E.; Kahn, M.L.; Noya, F.; J. Bacteriol. 191, 7581-7586 (2009)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
4.2.1.3
Fe4-S4 cluster
active enzyme contains an iron-sulfur cluster
Sinorhizobium meliloti
Cloned(Commentary)
EC Number
Commentary
Organism
4.2.1.3
expressed in Escherichia coli as a His-tagged fusion protein
Sinorhizobium meliloti
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.3
Sinorhizobium meliloti
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.3
using Ni-NTA chromatography
Sinorhizobium meliloti
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.3
isocitrate
-
704318
Sinorhizobium meliloti
cis-aconitate + H2O
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.3
7.4
-
assay at
Sinorhizobium meliloti
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
4.2.1.3
Fe4-S4 cluster
active enzyme contains an iron-sulfur cluster
Sinorhizobium meliloti
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.3
expressed in Escherichia coli as a His-tagged fusion protein
Sinorhizobium meliloti
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.3
using Ni-NTA chromatography
Sinorhizobium meliloti
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.3
isocitrate
-
704318
Sinorhizobium meliloti
cis-aconitate + H2O
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.3
7.4
-
assay at
Sinorhizobium meliloti
General Information
EC Number
General Information
Commentary
Organism
4.2.1.3
malfunction
acnA mutants grow very poorly, have secondary mutations, and are quickly outgrown by pseudorevertants. The acnA gene is stably interrupted in a citrate synthase (gltA) null background, indicating that the intracellular accumulation of citrate may be deleterious for survival. No aconitase activity is detected in this mutant. To uncover a function of AcnA beyond its catalytic role in the tricarboxylic acid cycle pathway, the citrate synthase/aconitase (acnA) double mutant is compared with the citrate synthase single mutant. No differences are found
Sinorhizobium meliloti
General Information (protein specific)
EC Number
General Information
Commentary
Organism
4.2.1.3
malfunction
acnA mutants grow very poorly, have secondary mutations, and are quickly outgrown by pseudorevertants. The acnA gene is stably interrupted in a citrate synthase (gltA) null background, indicating that the intracellular accumulation of citrate may be deleterious for survival. No aconitase activity is detected in this mutant. To uncover a function of AcnA beyond its catalytic role in the tricarboxylic acid cycle pathway, the citrate synthase/aconitase (acnA) double mutant is compared with the citrate synthase single mutant. No differences are found
Sinorhizobium meliloti