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Literature summary extracted from

  • Dong, S.; Chesnokova, O.N.; Turnbough, C.L.; Pritchard, D.G.
    Identification of the UDP-N-acetylglucosamine 4-epimerase involved in exosporium protein glycosylation in Bacillus anthracis (2009), J. Bacteriol., 191, 7094-7101.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.3.2 expression in Escherichia coli Bacillus anthracis
5.1.3.2 gene BAS5114, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3) Bacillus anthracis
5.1.3.7 expression in Escherichia coli Bacillus anthracis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.1.3.2 Mg2+
-
 Bacillus anthracis
5.1.3.2 Mg2+ 4 mM are included in assay medium Bacillus anthracis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.1.3.2 36900
-
 predicted by amino acid sequence Bacillus anthracis
5.1.3.2 37800
-
 predicted by amino acid sequence Bacillus anthracis
5.1.3.7 37800
-
 predicted by amino acid sequence Bacillus anthracis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.1.3.2 additional information Bacillus anthracis the gene encodes a bifunctional enzyme with both UDP-GlcNAc 4-epimerase and UDP-Glc 4-epimerase activities and that no other annotated UDP-Glc 4-epimerase gene encodes a UDP-GlcNAc 4-epimerase ?
-
 ?
5.1.3.2 additional information Bacillus anthracis UDP-N-acetylgalactoseamine 4-epimerase encoded by gene BAS5304 also shows UDP-glucose 4-epimerase activity, overview ?
-
 ?
5.1.3.2 UDP-alpha-D-glucose Bacillus anthracis
-
 UDP-alpha-D-galactose
-
 r
5.1.3.2 UDP-galactose Bacillus anthracis
-
 UDP-glucose
-
 r
5.1.3.7 additional information Bacillus anthracis the gene encodes a bifunctional enzyme with both UDP-GlcNAc 4-epimerase and UDP-Glc 4-epimerase activities and that no other annotated UDP-Glc 4-epimerase gene encodes a UDP-GlcNAc 4-epimerase ?
-
 ?
5.1.3.7 UDP-N-acetylgalactosamine Bacillus anthracis
-
 UDP-N-acetylglucosamine
-
 r

Organism

EC Number Organism UniProt Comment Textmining
5.1.3.2 Bacillus anthracis Q81JK4
-
-
5.1.3.2 Bacillus anthracis Q81K34
-
-
5.1.3.7 Bacillus anthracis Q81JK4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.3.2 by using nickel-nitrilotriacetic acid affinity chromatography and gel filtration Bacillus anthracis
5.1.3.2 recombinant enzyme from Escherichia coli strain BL21(DE3) Bacillus anthracis
5.1.3.7 by using nickel-nitrilotriacetic acid affinity chromatography and gel filtration Bacillus anthracis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.3.2 additional information the gene encodes a bifunctional enzyme with both UDP-GlcNAc 4-epimerase and UDP-Glc 4-epimerase activities and that no other annotated UDP-Glc 4-epimerase gene encodes a UDP-GlcNAc 4-epimerase Bacillus anthracis ?
-
 ?
5.1.3.2 additional information UDP-N-acetylgalactoseamine 4-epimerase encoded by gene BAS5304 also shows UDP-glucose 4-epimerase activity, overview Bacillus anthracis ?
-
 ?
5.1.3.2 additional information no activity with UDP-N-acetylgalactoseamine Bacillus anthracis ?
-
 ?
5.1.3.2 UDP-alpha-D-glucose
-
 Bacillus anthracis UDP-alpha-D-galactose
-
 r
5.1.3.2 UDP-galactose
-
 Bacillus anthracis UDP-glucose
-
 r
5.1.3.7 additional information the gene encodes a bifunctional enzyme with both UDP-GlcNAc 4-epimerase and UDP-Glc 4-epimerase activities and that no other annotated UDP-Glc 4-epimerase gene encodes a UDP-GlcNAc 4-epimerase Bacillus anthracis ?
-
 ?
5.1.3.7 UDP-N-acetylgalactosamine
-
 Bacillus anthracis UDP-N-acetylglucosamine
-
 r

Synonyms

EC Number Synonyms Comment Organism
5.1.3.2 UDP-Glc 4-epimerase
-
 Bacillus anthracis
5.1.3.2 UDP-GlcNAc 4-epimerase
-
 Bacillus anthracis
5.1.3.2 UDP-glucose 4-epimerase
-
 Bacillus anthracis
5.1.3.7 UDP-N-acetylglucosamine 4-epimerase
-
 Bacillus anthracis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.1.3.2 37
-
 assay at Bacillus anthracis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.1.3.2 100
-
 5 min, purified recombinant enzyme, inactivation Bacillus anthracis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1.3.2 8
-
 assay at Bacillus anthracis

Cofactor

EC Number Cofactor Comment Organism Structure
5.1.3.2 additional information contains an NAD+ binding site Bacillus anthracis
5.1.3.2 NAD+
-
 Bacillus anthracis

Expression

EC Number Organism Comment Expression
5.1.3.7 Bacillus anthracis spores produced by a mutant strain lacking the enzyme still contain normal levels of BclA-attached oligosaccharides, monosaccharide analysis of the oligosaccharides reveals that N-acetylglucosamine had replaced N-acetylgalactosamine, significant levels of gene transcripts are detected only during sporulation, an observation consistent with a role for this gene in exosporium assembly, gene expression during sporulation appears to be biphasic additional information

General Information

EC Number General Information Comment Organism
5.1.3.7 physiological function the epimerase is required to produce N-acetyl-galactosamine for BclA oligosaccharide biosynthesis, a collagen-like glycoprotein, UDP-GlcNAc 4-epimerase encoded by the BAS5304 gene is required for the synthesis of GalNAc in sporulating cells and that GlcNAc can replace GalNAc in the synthesis of BclA oligosaccharides Bacillus anthracis