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Literature summary extracted from

  • Zhang, Q.; Gao, F.; Peng, H.; Cheng, H.; Liu, Y.; Tang, J.; Thompson, J.; Wei, G.; Zhang, J.; Du, Y.; Yan, J.; Gao, G.F.
    Crystal structures of Streptococcus suis mannonate dehydratase (ManD) and its complex with substrate: genetic and biochemical evidence for a catalytic mechanism (2009), J. Bacteriol., 191, 5832-5837.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.8 expressed in Escherichia coli BL21(DE3) cells Streptococcus suis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.1.8 ManD in native form and in complex with its substrate D-mannonate and Mn2+ ion, hanging drop vapor diffusion method, using 0.2 M potassium-sodium tartrate, 0.1 M sodium citrate (pH 6.5), and 1 M ammonium sulfate (native form) or using 0.2 M potassium sodium tartrate tetrahydrate, 0.1 M trisodium citrate dehydrate (pH 6.5), 1 M ammonium sulfate, and 15 mM D-mannonate Streptococcus suis

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.8 H311A the activity of H311A is only 2.4% that of the native wild type enzyme Streptococcus suis
4.2.1.8 Y325F catalytically inactive Streptococcus suis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.8 1.9
-
 D-mannonate mutant enzyme H311A, at pH 7.5 and 37°C Streptococcus suis
4.2.1.8 3
-
 D-mannonate wild type enzyme, at pH 7.5 Streptococcus suis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.8 Mn2+ each ManD monomer roughly has one Mn2+ ion-binding site Streptococcus suis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.8 41000
-
 2 * 41000, SDS-PAGE Streptococcus suis
4.2.1.8 80000
-
 gel filtration Streptococcus suis

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.8 Streptococcus suis A4VVI4 serotype 2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.8 Ni-NTA column chromatography, Superdex 200 gel filtration Streptococcus suis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.8 D-Mannonate
-
 Streptococcus suis 2-Dehydro-3-deoxy-D-gluconate + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.8 homodimer 2 * 41000, SDS-PAGE Streptococcus suis

Synonyms

EC Number Synonyms Comment Organism
4.2.1.8 ManD
-
 Streptococcus suis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.1.8 4
-
 ManD exhibits no enzymatic activity at 4°C Streptococcus suis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.8 0.09
-
 D-mannonate mutant enzyme H311A, at pH 7.5 and 37°C Streptococcus suis
4.2.1.8 5.88
-
 D-mannonate wild type enzyme, at pH 7.5 and 37°C Streptococcus suis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.2.1.8 0.047
-
 D-mannonate mutant enzyme H311A, at pH 7.5 and 37°C Streptococcus suis
4.2.1.8 1.96
-
 D-mannonate wild type enzyme, at pH 7.5 and 37°C Streptococcus suis