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Literature summary extracted from

  • Bevers, L.E.; Pinkse, M.W.; Verhaert, P.D.; Hagen, W.R.
    Oleate hydratase catalyzes the hydration of a nonactivated carbon-carbon bond (2009), J. Bacteriol., 191, 5010-5012.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.1.53 additional information addition of EDTA or EGTA (both at a 1000fold excess) has no effect on the activity Elizabethkingia meningoseptica

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.53 into the pBAD/His A vector and expressed in Escherichia coli TOP10 cells Elizabethkingia meningoseptica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.53 0.078
-
 oleic acid at 22°C, in 20 mM Tris buffer, pH 8, 50 mM NaCl, with mixing speed of 2200 rpm, in the presence of 2.5% isopropyl alcoho Elizabethkingia meningoseptica
4.2.1.53 0.121
-
 oleic acid at 22°C, in 20 mM Tris buffer, pH 8, 50 mM NaCl, with mixing speed of 1400 rpm Elizabethkingia meningoseptica
4.2.1.53 0.21
-
 oleic acid at 30°C, in 20 mM Tris buffer, pH 8, 50 mM NaCl, with mixing speed of 1000 rpm Elizabethkingia meningoseptica
4.2.1.53 0.3
-
 oleic acid at 30°C, in 20 mM Tris buffer, pH 8, 50 mM NaCl, with mixing speed of 1400 rpm Elizabethkingia meningoseptica
4.2.1.53 0.56
-
 oleic acid at 22°C, in 20 mM Tris buffer, pH 8, 50 mM NaCl, with mixing speed of 2200 rpm Elizabethkingia meningoseptica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.53 additional information calcium has no role in the catalytic mechanism Elizabethkingia meningoseptica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.53 70000
-
 gel filtration Elizabethkingia meningoseptica
4.2.1.53 73487
-
 1 * 73487, calculated from sequence Elizabethkingia meningoseptica

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.53 Elizabethkingia meningoseptica C7DLJ6 formerly Pseudomonas sp. strain 3266
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.53 by three steps of gel filtration. Recombinant oleate hydratase fused to an N-terminal His tag purified onh Ni-resin Elizabethkingia meningoseptica

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.53 additional information
-
 specific activity of the enzyme does not change significantly in the presence of 2.5% or 5% isopropyl alcohol, but it decreases at isopropyl alcohol concentrations higher than 10% Elizabethkingia meningoseptica
4.2.1.53 0.14
-
 at 30°C, in 20 mM Tris buffer, pH 8, 50 mM NaCl, with mixing speed of 1400 rpm Elizabethkingia meningoseptica
4.2.1.53 0.16
-
 at 22°C, in 20 mM Tris buffer, pH 8, 50 mM NaCl, with mixing speed of 1400 rpm Elizabethkingia meningoseptica
4.2.1.53 0.17
-
 at 30°C, in 20 mM Tris buffer, pH 8, 50 mM NaCl, with mixing speed of 1000 rpm Elizabethkingia meningoseptica
4.2.1.53 0.34
-
 at 22°C, in 20 mM Tris buffer, pH 8, 50 mM NaCl, with mixing speed of 2200 rpm, in the presence of 2.5% isopropyl alcohol Elizabethkingia meningoseptica
4.2.1.53 0.39
-
 at 22°C, in 20 mM Tris buffer, pH 8, 50 mM NaCl, with mixing speed of 2200 rpm Elizabethkingia meningoseptica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.53 oleic acid + H2O
-
 Elizabethkingia meningoseptica 10-hydroxystearic acid
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.53 monomer 1 * 73487, calculated from sequence Elizabethkingia meningoseptica

Synonyms

EC Number Synonyms Comment Organism
4.2.1.53 OA hydratase
-
 Elizabethkingia meningoseptica
4.2.1.53 oleate hydratase
-
 Elizabethkingia meningoseptica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.53 6
-
-
 Elizabethkingia meningoseptica

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.1.53 5 7 pH 5: about 70% of maximal activity, pH 7: about about 75% of maximal activity Elizabethkingia meningoseptica

Expression

EC Number Organism Comment Expression
4.2.1.53 Elizabethkingia meningoseptica expression is strongly upregulated in cells grown in the presence of oleic acid up