BRENDA - Enzyme Database

Oxidation of cysteine 645 of cobalamin-independent methionine synthase causes a methionine limitation in Escherichia coli

Hondorp, E.R.; Matthews, R.G.; J. Bacteriol. 191, 3407-3410 (2009)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.1.1.14
expressed in Escherichia coli strain MTD23
Escherichia coli
Engineering
EC Number
Amino acid exchange
Commentary
Organism
2.1.1.14
C645A
the mutation confers resistance to diamide when cells are grown in media lacking methionine, but not when cells are grown in the presence of methionine, cysteine 645 serves to modulate the activity of MetE in vivo in response to disulfide stress
Escherichia coli
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.1.1.14
Diamide
-
Escherichia coli
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.1.1.14
Escherichia coli
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.14
5-methyltetrahydropteroyl-tri-L-glutamate + L-homocysteine
-
704294
Escherichia coli
tetrahydropteroyl-tri-L-glutamate + L-methionine
-
-
-
?
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.1.1.14
expressed in Escherichia coli strain MTD23
Escherichia coli
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
2.1.1.14
C645A
the mutation confers resistance to diamide when cells are grown in media lacking methionine, but not when cells are grown in the presence of methionine, cysteine 645 serves to modulate the activity of MetE in vivo in response to disulfide stress
Escherichia coli
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.1.1.14
Diamide
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.14
5-methyltetrahydropteroyl-tri-L-glutamate + L-homocysteine
-
704294
Escherichia coli
tetrahydropteroyl-tri-L-glutamate + L-methionine
-
-
-
?