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Literature summary extracted from

  • Hyun, Y.J.; Lee, K.S.; Kim, D.H.
    Cloning, expression and characterization of acharan sulfate-degrading heparin lyase II from Bacteroides stercoris HJ-15 (2010), J. Appl. Microbiol., 108, 226-235.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.2.7 expressed in Escherichia coli BL21(DE3) Bacteroides stercoris

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.7 Co2+
-
 Bacteroides stercoris
4.2.2.7 Cu2+
-
 Bacteroides stercoris
4.2.2.7 Ni2+
-
 Bacteroides stercoris
4.2.2.7 Pb2+
-
 Bacteroides stercoris
4.2.2.7 Zn2+
-
 Bacteroides stercoris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.2.7 additional information
-
 heparan sulfate 0.0000182 g/ml Bacteroides stercoris
4.2.2.7 additional information
-
 heparin 0.00002095 g/ml Bacteroides stercoris
4.2.2.7 additional information
-
 acharan sulfate 0.00003799 g/ml Bacteroides stercoris

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.2.7 Ba2+
-
 Bacteroides stercoris
4.2.2.7 Ca2+
-
 Bacteroides stercoris
4.2.2.7 Mg2+
-
 Bacteroides stercoris
4.2.2.7 Mn2+
-
 Bacteroides stercoris
4.2.2.7 additional information optimal condition: 75 mmol/l NaCl Bacteroides stercoris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.7 85400
-
 x * 85400, calculated Bacteroides stercoris

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.7 Bacteroides stercoris C0JBW5
-
-
4.2.2.7 Bacteroides stercoris HJ-15 C0JBW5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.7 ion exchange chromatography Bacteroides stercoris

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.2.7 additional information
-
 no activity with chondroitin sulfate or hyaluronic acid Bacteroides stercoris
4.2.2.7 6.38
-
 heparin Bacteroides stercoris
4.2.2.7 27.74
-
 heparan sulfate Bacteroides stercoris
4.2.2.7 109.9
-
 acharan sulfate Bacteroides stercoris

Storage Stability

EC Number Storage Stability Organism
4.2.2.7 4°C, without any additives, 15 days, retains its activity, more stable at 4°C than at -20°C, activity decreases after a few cycles of freezing and thawing Bacteroides stercoris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.7 acharan sulfate uniformly repeating disaccharide structure of alpha-D-N-acetylglucosaminyl-2-O-sulfo-alpha-L-iduronic acid Bacteroides stercoris n 4-deoxy-2-O-sulfo-alpha-L-threo-hex-4-enopyranosyl-(1->4)-N-acetyl-D-glucosamine
-
 ?
4.2.2.7 acharan sulfate uniformly repeating disaccharide structure of alpha-D-N-acetylglucosaminyl-2-O-sulfo-alpha-L-iduronic acid Bacteroides stercoris HJ-15 n 4-deoxy-2-O-sulfo-alpha-L-threo-hex-4-enopyranosyl-(1->4)-N-acetyl-D-glucosamine
-
 ?
4.2.2.7 heparan sulfate
-
 Bacteroides stercoris ? 5 products found, major product not identified ?
4.2.2.7 heparan sulfate
-
 Bacteroides stercoris HJ-15 ? 5 products found, major product not identified ?
4.2.2.7 heparin
-
 Bacteroides stercoris n 4-deoxy-2-O-sulfo-alpha-L-threo-hex-4-enopyranosyl-(1->4)-N-acetyl-6-O-sulfo-D-glucosamine + ? 2 minor products found ?
4.2.2.7 heparin
-
 Bacteroides stercoris HJ-15 n 4-deoxy-2-O-sulfo-alpha-L-threo-hex-4-enopyranosyl-(1->4)-N-acetyl-6-O-sulfo-D-glucosamine + ? 2 minor products found ?

Subunits

EC Number Subunits Comment Organism
4.2.2.7 ? x * 85400, calculated Bacteroides stercoris

Synonyms

EC Number Synonyms Comment Organism
4.2.2.7 heparin lyase II
-
 Bacteroides stercoris
4.2.2.7 heparinase II
-
 Bacteroides stercoris

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.7 32
-
-
 Bacteroides stercoris

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.2.2.7 20 37
-
 Bacteroides stercoris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.7 7.4
-
 substrate heparin Bacteroides stercoris

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.2.7 5 8
-
 Bacteroides stercoris

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.2.2.7 Bacteroides stercoris calculated for mature protein
-
 7.35