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Literature summary extracted from

  • Zhang, Y.; Luo, M.; Schramm, V.L.
    Transition states of Plasmodium falciparum and human orotate phosphoribosyltransferases (2009), J. Am. Chem. Soc., 131, 4685-4694.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.10 expressed from pDEST14-OPRT in Escherichia coli BL21-AI competent cells Homo sapiens
2.4.2.10 into the pDONR221 vector, expressed from pDEST14-OPRT in Escherichia coli BL21-AI competent cells Plasmodium falciparum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.10 p-nitrophenyl beta-D-ribose
-
Homo sapiens
2.4.2.10 p-nitrophenyl beta-D-ribose
-
Plasmodium falciparum
2.4.2.10 p-nitrophenyl beta-D-ribose 5'-phosphate competitive inhibitor against orotidine 5'-phosphate Homo sapiens
2.4.2.10 p-nitrophenyl beta-D-ribose 5'-phosphate competitive inhibitor against orotidine 5'-phosphate Plasmodium falciparum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.10 0.00155
-
orotidine 5'-phosphate in the presence of 1 mM diphosphate, at 25°C, 50 mM Tris-HCl, pH 8.0, 5 mM MgCl2 Homo sapiens
2.4.2.10 0.00214
-
orotidine 5'-phosphate in the presence of 20 mM phosphonoacetic acid, at 25°C, 50 mM Tris-HCl, pH 8.0, 5 mM MgCl2 Homo sapiens
2.4.2.10 0.0029
-
orotidine 5'-phosphate in the presence of 20 mM phosphonoacetic acid, at 25°C, 50 mM Tris-HCl, pH 8.0, 5 mM MgCl2 Plasmodium falciparum
2.4.2.10 0.00373
-
orotidine 5'-phosphate in the presence of 1 mM diphosphate, at 25°C, 50 mM Tris-HCl, pH 8.0, 5 mM MgCl2 Plasmodium falciparum
2.4.2.10 0.091
-
Orotidine
-
Homo sapiens
2.4.2.10 0.096
-
Orotidine
-
Plasmodium falciparum
2.4.2.10 1.59
-
Phosphonoacetic acid
-
Plasmodium falciparum
2.4.2.10 3.84
-
Phosphonoacetic acid
-
Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.2.10 Mg2+
-
Homo sapiens
2.4.2.10 Mg2+
-
Plasmodium falciparum

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.10 Homo sapiens
-
-
-
2.4.2.10 Plasmodium falciparum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.10 Ni2+ affinity column Homo sapiens
2.4.2.10 Ni2+ affinity column Plasmodium falciparum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.10 orotate + 5-phospho-alpha-D-ribose 1-diphosphate
-
Homo sapiens orotidine 5'-phosphate + diphosphate
-
?
2.4.2.10 orotate + 5-phospho-alpha-D-ribose 1-diphosphate
-
Plasmodium falciparum orotidine 5'-phosphate + diphosphate
-
?
2.4.2.10 orotidine 5'-phosphate + diphosphate
-
Homo sapiens orotate + 5-phospho-alpha-D-ribose 1-diphosphate
-
?
2.4.2.10 orotidine 5'-phosphate + diphosphate
-
Plasmodium falciparum orotate + 5-phospho-alpha-D-ribose 1-diphosphate
-
?
2.4.2.10 orotidine 5'-phosphate + phosphonoacetic acid
-
Homo sapiens ?
-
?
2.4.2.10 orotidine 5'-phosphate + phosphonoacetic acid
-
Plasmodium falciparum ?
-
?

Synonyms

EC Number Synonyms Comment Organism
2.4.2.10 OPRT
-
Homo sapiens
2.4.2.10 OPRT
-
Plasmodium falciparum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.2.10 0.014
-
orotidine 5'-phosphate in the presence of 20 mM phosphonoacetic acid, at 25°C, 50 mM Tris-HCl, pH 8.0, 5 mM MgCl2 Plasmodium falciparum
2.4.2.10 0.017
-
Phosphonoacetic acid
-
Homo sapiens
2.4.2.10 0.017
-
orotidine 5'-phosphate in the presence of 20 mM phosphonoacetic acid, at 25°C, 50 mM Tris-HCl, pH 8.0, 5 mM MgCl2 Homo sapiens
2.4.2.10 0.023
-
Phosphonoacetic acid
-
Plasmodium falciparum
2.4.2.10 0.024
-
Orotidine
-
Homo sapiens
2.4.2.10 0.042
-
Orotidine
-
Plasmodium falciparum
2.4.2.10 0.1
-
orotidine 5'-phosphate in the presence of 1 mM diphosphate, at 25°C, 50 mM Tris-HCl, pH 8.0, 5 mM MgCl2 Homo sapiens
2.4.2.10 1.96
-
orotidine 5'-phosphate in the presence of 1 mM diphosphate, at 25°C, 50 mM Tris-HCl, pH 8.0, 5 mM MgCl2 Plasmodium falciparum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.2.10 0.00004
-
p-nitrophenyl beta-D-ribose 5'-phosphate in the presence of orotidine 5'-phosphate, at 25°C, 50 mM Tris-HCl, pH 8.0, 5 mM MgCl2 Plasmodium falciparum
2.4.2.10 0.000041
-
p-nitrophenyl beta-D-ribose 5'-phosphate in the presence of orotidine 5'-phosphate, at 25°C, 50 mM Tris-HCl, pH 8.0, 5 mM MgCl2 Homo sapiens
2.4.2.10 0.000121
-
p-nitrophenyl beta-D-ribose in the presence of orotidine 5'-phosphate, at 25°C, 50 mM Tris-HCl, pH 8.0, 5 mM MgCl2 Homo sapiens
2.4.2.10 0.000188
-
p-nitrophenyl beta-D-ribose in the presence of orotidine 5'-phosphate, at 25°C, 50 mM Tris-HCl, pH 8.0, 5 mM MgCl2 Plasmodium falciparum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.2.10 4.8
-
orotidine 5'-phosphate in the presence of 20 mM phosphonoacetic acid, at 25°C, 50 mM Tris-HCl, pH 8.0, 5 mM MgCl2 Plasmodium falciparum
2.4.2.10 7.9
-
orotidine 5'-phosphate in the presence of 20 mM phosphonoacetic acid, at 25°C, 50 mM Tris-HCl, pH 8.0, 5 mM MgCl2 Homo sapiens
2.4.2.10 65
-
orotidine 5'-phosphate in the presence of 1 mM diphosphate, at 25°C, 50 mM Tris-HCl, pH 8.0, 5 mM MgCl2 Homo sapiens
2.4.2.10 530
-
orotidine 5'-phosphate in the presence of 1 mM diphosphate, at 25°C, 50 mM Tris-HCl, pH 8.0, 5 mM MgCl2 Plasmodium falciparum