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Literature summary extracted from

  • Puthenveetil, S.; Liu, D.S.; White, K.A.; Thompson, S.; Ting, A.Y.
    Yeast display evolution of a kinetically efficient 13-amino acid substrate for lipoic acid ligase (2009), J. Am. Chem. Soc., 131, 16430-16438.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.1.20 0.2
-
 LplA acceptor peptide 1
-
 Escherichia coli
6.3.1.20 13.32
-
 LplA acceptor peptide 2
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.1.20 ATP + lipoate + H-protein Escherichia coli
-
 AMP + diphosphate + ?
-
 ?
6.3.1.20 additional information Escherichia coli LplA's natural protein substrates have a conserved beta-hairpin structure ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.1.20 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.1.20 ATP + lipoate + biotin
-
 Escherichia coli AMP + diphosphate + ?
-
 ?
6.3.1.20 ATP + lipoate + H-protein
-
 Escherichia coli AMP + diphosphate + ?
-
 ?
6.3.1.20 ATP + lipoate + LplA acceptor peptide 1
-
 Escherichia coli ?
-
 ?
6.3.1.20 ATP + lipoate + LplA acceptor peptide 2
-
 Escherichia coli ?
-
 ?
6.3.1.20 additional information LplA's natural protein substrates have a conserved beta-hairpin structure Escherichia coli ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.1.20 lipoic acid ligase
-
 Escherichia coli
6.3.1.20 LPLA
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3.1.20 0.22
-
 LplA acceptor peptide 2
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.1.20 ATP
-
 Escherichia coli

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.3.1.20 0.0000165
-
 LplA acceptor peptide 2
-
 Escherichia coli