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Literature summary extracted from

  • Luk, L.Y.; Tanner, M.E.
    Mechanism of dimethylallyltryptophan synthase: evidence for a dimethylallyl cation intermediate in an aromatic prenyltransferase reaction (2009), J. Am. Chem. Soc., 131, 13932-13933.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.34 overexpressed in Escherichia coli as a C-terminal His-tagged fusion protein Aspergillus fumigatus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.34 EDTA 3 mM EDTA leads to a 2fold reduction Aspergillus fumigatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.34 0.01
-
 dimethylallyl diphosphate
-
 Aspergillus fumigatus
2.5.1.34 0.011
-
 L-tryptophan
-
 Aspergillus fumigatus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.34 additional information the enzyme does not require exogenous divalent metal ions for activity Aspergillus fumigatus

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.34 Aspergillus fumigatus
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.5.1.34 dimethylallyl diphosphate + L-tryptophan = diphosphate + 4-(3-methylbut-2-enyl)-L-tryptophan studies are presented that strongly support the dissociative mechanism for the DMAT synthase reaction. The observation of positional isotope exchange (PIX) in isotopically labeled DMAPP provides evidence for the existence of a dimethylallyl carbocation intermediate Aspergillus fumigatus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.34 dimethylallyl diphosphate + L-tryptophan
-
 Aspergillus fumigatus diphosphate + 4-dimethylallyl-L-tryptophan
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.34 dimethylallyltryptophan synthase
-
 Aspergillus fumigatus
2.5.1.34 DMAT synthase
-
 Aspergillus fumigatus