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Literature summary extracted from

  • Hadler, K.S.; Tanifum, E.A.; Yip, S.H.; Mitic, N.; Guddat, L.W.; Jackson, C.J.; Gahan, L.R.; Nguyen, K.; Carr, P.D.; Ollis, D.L.; Hengge, A.C.; Larrabee, J.A.; Schenk, G.
    Substrate-promoted formation of a catalytically competent binuclear center and regulation of reactivity in a glycerophosphodiesterase from Enterobacter aerogenes (2008), J. Am. Chem. Soc., 130, 14129-14138.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.46 expressed in Escherichia coli DH5alpha Klebsiella aerogenes

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.4.46
-
 Klebsiella aerogenes

Protein Variants

EC Number Protein Variants Comment Organism
3.1.4.46 N80A ligand of the metal ion Klebsiella aerogenes
3.1.4.46 N80D ligand of the metal ion Klebsiella aerogenes

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.46 p-nitrophenyl phosphate
-
 Klebsiella aerogenes
3.1.4.46 phosphate hydrolysis of p-nitrophenyl phosphate, competitive Klebsiella aerogenes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.46 0.12
-
 p-nitrophenyl phosphate wild-type protein, pH 7.0, 1 mM CoCl2 Klebsiella aerogenes
3.1.4.46 39
-
 p-nitrophenyl phosphate N80D mutant protein, pH 7.0, 1 mM CoCl2 Klebsiella aerogenes
3.1.4.46 49
-
 p-nitrophenyl phosphate N80A mutant protein, pH 7.0, 1 mM CoCl2 Klebsiella aerogenes

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.46 additional information a Co(II)-substituted enzyme is used Klebsiella aerogenes

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.46 31000
-
 x * 31000, SDS-PAGE Klebsiella aerogenes

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.46 Klebsiella aerogenes Q6XBH1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.46 ion-exchange chromatography (DEAE), hydrophobic interaction chromatography (Phenyl Sepharose), gel filtration Klebsiella aerogenes

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.46 bis(p-nitrophenyl) phosphate + H2O
-
 Klebsiella aerogenes p-nitrophenyl phosphate + p-nitrophenol
-
 ?
3.1.4.46 ethyl p-nitrophenyl phosphate + H2O
-
 Klebsiella aerogenes p-nitrophenol + ethyl phosphate
-
 ?
3.1.4.46 p-nitrophenyl phosphate + H2O
-
 Klebsiella aerogenes p-nitrophenol + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.4.46 ? x * 31000, SDS-PAGE Klebsiella aerogenes

Synonyms

EC Number Synonyms Comment Organism
3.1.4.46 glycerophosphodiesterase
-
 Klebsiella aerogenes

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.4.46 0.24
-
 p-nitrophenyl phosphate N80D mutant protein, pH 7.0, 1 mM CoCl2 Klebsiella aerogenes
3.1.4.46 1.89
-
 p-nitrophenyl phosphate wild-type protein, pH 7.0, 1 mM CoCl2 Klebsiella aerogenes
3.1.4.46 6.4
-
 p-nitrophenyl phosphate N80A mutant protein, pH 7.0, 1 mM CoCl2 Klebsiella aerogenes

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.4.46 0.078
-
 p-nitrophenyl phosphate
-
 Klebsiella aerogenes

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.4.46 0.006
-
 p-nitrophenyl phosphate N80D mutant protein, pH 7.0, 1 mM CoCl2 Klebsiella aerogenes
3.1.4.46 0.13
-
 p-nitrophenyl phosphate N80A mutant protein, pH 7.0, 1 mM CoCl2 Klebsiella aerogenes
3.1.4.46 16.4
-
 p-nitrophenyl phosphate wild-type protein, pH 7.0, 1 mM CoCl2 Klebsiella aerogenes