BRENDA - Enzyme Database show

Functional characterization of two 13-lipoxygenase genes from olive fruit in relation to the biosynthesis of volatile compounds of virgin olive oil

Padilla, M.N.; Hernandez, M.L.; Sanz, C.; Martnez-Rivas, J.M.; J. Agric. Food Chem. 57, 9097-9107 (2009)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.13.11.12
isoenzyme Oep1LOX2 and Oep2LOX2; Oep1LOX2 and Oep2LOX2 ligated into vector pQE-80 L and overexpressed in Escherichia coli strain XL1-Blue
Olea europaea
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.13.11.12
Cu2+
inhibits Oep2LOX2 by 49%
Olea europaea
1.13.11.12
Fe3+
slight inactivation
Olea europaea
1.13.11.12
Hg2+
inhibits Oep1LOX2 by 60% and totally inactivates Oep2LOX2
Olea europaea
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.13.11.12
0.01459
-
linoleic acid
isoform Oep1LOX2
Olea europaea
1.13.11.12
0.0146
-
linoleate
pH 6.5, 20C, isoenzyme Oep1LOX2
Olea europaea
1.13.11.12
0.028
-
alpha-linolenate
pH 6.5, 20C, isoenzyme Oep1LOX2
Olea europaea
1.13.11.12
0.028
-
linolenic acid
isoform Oep1LOX2
Olea europaea
1.13.11.12
0.1297
-
linoleic acid
isoform Oep2LOX2
Olea europaea
1.13.11.12
0.13
-
linoleate
pH 6.5, 20C, isoenzyme Oep2LOX2
Olea europaea
1.13.11.12
0.1389
-
linolenic acid
isoform Oep2LOX2
Olea europaea
1.13.11.12
0.14
-
alpha-linolenate
pH 6.5, 20C, isoenzyme Oep2LOX2
Olea europaea
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.13.11.12
chloroplast
-
Olea europaea
9507
-
1.13.11.12
additional information
isoenzyme Oep1LOX2 and Oep2LOX2 contain an N-terminal chloroplastic transit peptide
Olea europaea
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.13.11.12
Fe3+
among the conserved amino acids in sequences of both isozymes are three His, one Asn, and one Ile residue, which are essential for the binding of the atom of iron in the active site of the LOXs enzymes
Olea europaea
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.13.11.12
101700
-
sequence analysis, isoform Oep2LOX2
Olea europaea
1.13.11.12
103400
-
sequence analysis, isoform Oep1LOX2
Olea europaea
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.13.11.12
Olea europaea
-
; cv. Picual
-
Purification (Commentary)
EC Number
Commentary
Organism
1.13.11.12
by affinity chromatography; isoenzyme Oep1LOX2 and Oep2LOX2
Olea europaea
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.13.11.12
fruit
-
Olea europaea
-
1.13.11.12
leaf
Oep1LOX2 predominantely expressed
Olea europaea
-
1.13.11.12
mesocarp
Oep2LOX2 shows the highest expression levels in mature mesocarp tissue
Olea europaea
-
1.13.11.12
seed
Oep2LOX2 during development and ripening of Picual and Arbequina olive fruit
Olea europaea
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.13.11.12
alpha-linolenate + O2
isoenzyme Oep1LOX2 and Oep2LOX2 both produce primarily 13-hydroperoxides from linoleic acid and linolenic acid. Linolenic acid is the preferred substrate for both isoenzymes (Vmax/KM is about 10fold higher for linolenic acid)
704142
Olea europaea
(9Z,11E,15Z)-13-hydroperoxy-9,11,15-octadecatrienoate
-
-
-
?
1.13.11.12
linoleate + O2
isoenzyme Oep1LOX2 and Oep2LOX2 both produce primarily 13-hydroperoxides from linoleic acid and linolenic acid. Linolenic acid is the preferred substrate for both isoenzymes (Vmax/KM is about 10fold higher for linolenic acid)
704142
Olea europaea
(9Z,11E)-13-hydroperoxy-9,11-octadecadienoate
-
-
-
?
1.13.11.12
linoleic acid + O2
preferred substrate
704142
Olea europaea
(9Z,11E)-(13S)-13-hydroperoxyoctadeca-9,11-dienoic acid
-
-
-
?
1.13.11.12
linolenic acid + O2
-
704142
Olea europaea
?
-
-
-
?
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.13.11.12
35
-
isoenzyme Oep2LOX2; isoform Oep2LOX2
Olea europaea
1.13.11.12
45
-
isoenzyme Oep1LOX2; isoform Oep1LOX2
Olea europaea
Temperature Range [C]
EC Number
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
1.13.11.12
15
55
more than 85% of maximal activity in the temperature-range: 15-55C, isoenzyme Oep1LOX2; recombinant Oep1LOX2 exhibits more than 85% of its maximal activity, whereas Oep2LOX2 displays about 50-100% of its maximal activity
Olea europaea
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.13.11.12
6.25
-
isoform Oep1LOX2
Olea europaea
1.13.11.12
6.3
-
isoenzyme Oep2LOX2
Olea europaea
1.13.11.12
6.75
-
isoform Oep2LOX2
Olea europaea
1.13.11.12
6.8
-
isoenzyme Oep1LOX2
Olea europaea
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
1.13.11.12
Olea europaea
sequence analysis, isoform Oep2LOX2
-
5.9
1.13.11.12
Olea europaea
sequence analysis, isoform Oep1LOX2
-
7.2
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.13.11.12
isoenzyme Oep1LOX2 and Oep2LOX2; Oep1LOX2 and Oep2LOX2 ligated into vector pQE-80 L and overexpressed in Escherichia coli strain XL1-Blue
Olea europaea
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.13.11.12
Cu2+
inhibits Oep2LOX2 by 49%
Olea europaea
1.13.11.12
Fe3+
slight inactivation
Olea europaea
1.13.11.12
Hg2+
inhibits Oep1LOX2 by 60% and totally inactivates Oep2LOX2
Olea europaea
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.13.11.12
0.01459
-
linoleic acid
isoform Oep1LOX2
Olea europaea
1.13.11.12
0.0146
-
linoleate
pH 6.5, 20C, isoenzyme Oep1LOX2
Olea europaea
1.13.11.12
0.028
-
alpha-linolenate
pH 6.5, 20C, isoenzyme Oep1LOX2
Olea europaea
1.13.11.12
0.028
-
linolenic acid
isoform Oep1LOX2
Olea europaea
1.13.11.12
0.1297
-
linoleic acid
isoform Oep2LOX2
Olea europaea
1.13.11.12
0.13
-
linoleate
pH 6.5, 20C, isoenzyme Oep2LOX2
Olea europaea
1.13.11.12
0.1389
-
linolenic acid
isoform Oep2LOX2
Olea europaea
1.13.11.12
0.14
-
alpha-linolenate
pH 6.5, 20C, isoenzyme Oep2LOX2
Olea europaea
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.13.11.12
chloroplast
-
Olea europaea
9507
-
1.13.11.12
additional information
isoenzyme Oep1LOX2 and Oep2LOX2 contain an N-terminal chloroplastic transit peptide
Olea europaea
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.13.11.12
Fe3+
among the conserved amino acids in sequences of both isozymes are three His, one Asn, and one Ile residue, which are essential for the binding of the atom of iron in the active site of the LOXs enzymes
Olea europaea
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.13.11.12
101700
-
sequence analysis, isoform Oep2LOX2
Olea europaea
1.13.11.12
103400
-
sequence analysis, isoform Oep1LOX2
Olea europaea
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.13.11.12
by affinity chromatography; isoenzyme Oep1LOX2 and Oep2LOX2
Olea europaea
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.13.11.12
fruit
-
Olea europaea
-
1.13.11.12
leaf
Oep1LOX2 predominantely expressed
Olea europaea
-
1.13.11.12
mesocarp
Oep2LOX2 shows the highest expression levels in mature mesocarp tissue
Olea europaea
-
1.13.11.12
seed
Oep2LOX2 during development and ripening of Picual and Arbequina olive fruit
Olea europaea
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.13.11.12
alpha-linolenate + O2
isoenzyme Oep1LOX2 and Oep2LOX2 both produce primarily 13-hydroperoxides from linoleic acid and linolenic acid. Linolenic acid is the preferred substrate for both isoenzymes (Vmax/KM is about 10fold higher for linolenic acid)
704142
Olea europaea
(9Z,11E,15Z)-13-hydroperoxy-9,11,15-octadecatrienoate
-
-
-
?
1.13.11.12
linoleate + O2
isoenzyme Oep1LOX2 and Oep2LOX2 both produce primarily 13-hydroperoxides from linoleic acid and linolenic acid. Linolenic acid is the preferred substrate for both isoenzymes (Vmax/KM is about 10fold higher for linolenic acid)
704142
Olea europaea
(9Z,11E)-13-hydroperoxy-9,11-octadecadienoate
-
-
-
?
1.13.11.12
linoleic acid + O2
preferred substrate
704142
Olea europaea
(9Z,11E)-(13S)-13-hydroperoxyoctadeca-9,11-dienoic acid
-
-
-
?
1.13.11.12
linolenic acid + O2
-
704142
Olea europaea
?
-
-
-
?
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.13.11.12
35
-
isoenzyme Oep2LOX2; isoform Oep2LOX2
Olea europaea
1.13.11.12
45
-
isoenzyme Oep1LOX2; isoform Oep1LOX2
Olea europaea
Temperature Range [C] (protein specific)
EC Number
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
1.13.11.12
15
55
more than 85% of maximal activity in the temperature-range: 15-55C, isoenzyme Oep1LOX2; recombinant Oep1LOX2 exhibits more than 85% of its maximal activity, whereas Oep2LOX2 displays about 50-100% of its maximal activity
Olea europaea
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.13.11.12
6.25
-
isoform Oep1LOX2
Olea europaea
1.13.11.12
6.3
-
isoenzyme Oep2LOX2
Olea europaea
1.13.11.12
6.75
-
isoform Oep2LOX2
Olea europaea
1.13.11.12
6.8
-
isoenzyme Oep1LOX2
Olea europaea
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
1.13.11.12
Olea europaea
sequence analysis, isoform Oep2LOX2
-
5.9
1.13.11.12
Olea europaea
sequence analysis, isoform Oep1LOX2
-
7.2
Expression
EC Number
Organism
Commentary
Expression
1.13.11.12
Olea europaea
Oep2LOX2 transcript levels increase in Picual and Arbequina mesocarps during development and ripening, showing a maximum at turning stage, whereas Oep1LOX2 exhibits constant expression levels in both olive varieties and tissues
up
General Information
EC Number
General Information
Commentary
Organism
1.13.11.12
physiological function
major involvement of the Oep2LOX2 gene in the biosynthesis of virgin olive oil aroma compounds
Olea europaea
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.13.11.12
physiological function
major involvement of the Oep2LOX2 gene in the biosynthesis of virgin olive oil aroma compounds
Olea europaea
Expression (protein specific)
EC Number
Organism
Commentary
Expression
1.13.11.12
Olea europaea
Oep2LOX2 transcript levels increase in Picual and Arbequina mesocarps during development and ripening, showing a maximum at turning stage, whereas Oep1LOX2 exhibits constant expression levels in both olive varieties and tissues
up
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.13.11.12
additional information
-
additional information
linolenic acid is the preferred substrate for both isoenzymes (Vmax/KM is about 10fold higher for linolenic acid)
Olea europaea
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.13.11.12
additional information
-
additional information
linolenic acid is the preferred substrate for both isoenzymes (Vmax/KM is about 10fold higher for linolenic acid)
Olea europaea