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Literature summary extracted from

  • Padilla, M.N.; Hernandez, M.L.; Sanz, C.; Martínez-Rivas, J.M.
    Functional characterization of two 13-lipoxygenase genes from olive fruit in relation to the biosynthesis of volatile compounds of virgin olive oil (2009), J. Agric. Food Chem., 57, 9097-9107.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.12 isoenzyme Oep1LOX2 and Oep2LOX2 Olea europaea
1.13.11.12 Oep1LOX2 and Oep2LOX2 ligated into vector pQE-80 L and overexpressed in Escherichia coli strain XL1-Blue Olea europaea

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.12 Cu2+ inhibits Oep2LOX2 by 49% Olea europaea
1.13.11.12 Fe3+ slight inactivation Olea europaea
1.13.11.12 Hg2+ inhibits Oep1LOX2 by 60% and totally inactivates Oep2LOX2 Olea europaea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.12 0.01459
-
 linoleic acid isoform Oep1LOX2 Olea europaea
1.13.11.12 0.0146
-
 linoleate pH 6.5, 20°C, isoenzyme Oep1LOX2 Olea europaea
1.13.11.12 0.028
-
 linolenic acid isoform Oep1LOX2 Olea europaea
1.13.11.12 0.028
-
 alpha-linolenate pH 6.5, 20°C, isoenzyme Oep1LOX2 Olea europaea
1.13.11.12 0.1297
-
 linoleic acid isoform Oep2LOX2 Olea europaea
1.13.11.12 0.13
-
 linoleate pH 6.5, 20°C, isoenzyme Oep2LOX2 Olea europaea
1.13.11.12 0.1389
-
 linolenic acid isoform Oep2LOX2 Olea europaea
1.13.11.12 0.14
-
 alpha-linolenate pH 6.5, 20°C, isoenzyme Oep2LOX2 Olea europaea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.13.11.12 chloroplast
-
 Olea europaea 9507
-
1.13.11.12 additional information isoenzyme Oep1LOX2 and Oep2LOX2 contain an N-terminal chloroplastic transit peptide Olea europaea
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.12 Fe3+ among the conserved amino acids in sequences of both isozymes are three His, one Asn, and one Ile residue, which are essential for the binding of the atom of iron in the active site of the LOXs enzymes Olea europaea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.12 101700
-
 sequence analysis, isoform Oep2LOX2 Olea europaea
1.13.11.12 103400
-
 sequence analysis, isoform Oep1LOX2 Olea europaea

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.12 Olea europaea
-
-
-
1.13.11.12 Olea europaea
-
 cv. Picual
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.12 by affinity chromatography Olea europaea
1.13.11.12 isoenzyme Oep1LOX2 and Oep2LOX2 Olea europaea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.12 fruit
-
 Olea europaea
-
1.13.11.12 leaf Oep1LOX2 predominantely expressed Olea europaea
-
1.13.11.12 mesocarp Oep2LOX2 shows the highest expression levels in mature mesocarp tissue Olea europaea
-
1.13.11.12 seed Oep2LOX2 during development and ripening of Picual and Arbequina olive fruit Olea europaea
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.12 alpha-linolenate + O2 isoenzyme Oep1LOX2 and Oep2LOX2 both produce primarily 13-hydroperoxides from linoleic acid and linolenic acid. Linolenic acid is the preferred substrate for both isoenzymes (Vmax/KM is about 10fold higher for linolenic acid) Olea europaea (9Z,11E,15Z)-13-hydroperoxy-9,11,15-octadecatrienoate
-
 ?
1.13.11.12 linoleate + O2 isoenzyme Oep1LOX2 and Oep2LOX2 both produce primarily 13-hydroperoxides from linoleic acid and linolenic acid. Linolenic acid is the preferred substrate for both isoenzymes (Vmax/KM is about 10fold higher for linolenic acid) Olea europaea (9Z,11E)-13-hydroperoxy-9,11-octadecadienoate
-
 ?
1.13.11.12 linoleic acid + O2 preferred substrate Olea europaea (9Z,11E)-(13S)-13-hydroperoxyoctadeca-9,11-dienoic acid
-
 ?
1.13.11.12 linolenic acid + O2
-
 Olea europaea ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.12 13-lipoxygenase
-
 Olea europaea
1.13.11.12 LOX2
-
 Olea europaea
1.13.11.12 Oep1LOX2
-
 Olea europaea
1.13.11.12 Oep2LOX2
-
 Olea europaea

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.12 35
-
 isoenzyme Oep2LOX2 Olea europaea
1.13.11.12 35
-
 isoform Oep2LOX2 Olea europaea
1.13.11.12 45
-
 isoenzyme Oep1LOX2 Olea europaea
1.13.11.12 45
-
 isoform Oep1LOX2 Olea europaea

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.13.11.12 15 55 more than 85% of maximal activity in the temperature-range: 15-55°C, isoenzyme Oep1LOX2 Olea europaea
1.13.11.12 15 55 recombinant Oep1LOX2 exhibits more than 85% of its maximal activity, whereas Oep2LOX2 displays about 50-100% of its maximal activity Olea europaea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.12 6.25
-
 isoform Oep1LOX2 Olea europaea
1.13.11.12 6.3
-
 isoenzyme Oep2LOX2 Olea europaea
1.13.11.12 6.75
-
 isoform Oep2LOX2 Olea europaea
1.13.11.12 6.8
-
 isoenzyme Oep1LOX2 Olea europaea

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.13.11.12 Olea europaea sequence analysis, isoform Oep2LOX2
-
 5.9
1.13.11.12 Olea europaea sequence analysis, isoform Oep1LOX2
-
 7.2

Expression

EC Number Organism Comment Expression
1.13.11.12 Olea europaea Oep2LOX2 transcript levels increase in Picual and Arbequina mesocarps during development and ripening, showing a maximum at turning stage, whereas Oep1LOX2 exhibits constant expression levels in both olive varieties and tissues up

General Information

EC Number General Information Comment Organism
1.13.11.12 physiological function major involvement of the Oep2LOX2 gene in the biosynthesis of virgin olive oil aroma compounds Olea europaea

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.13.11.12 additional information
-
 additional information linolenic acid is the preferred substrate for both isoenzymes (Vmax/KM is about 10fold higher for linolenic acid) Olea europaea