Any feedback?
Literature summary extracted from
- Cloning, expression, and characterization of an enzyme possessing both glutaredoxin and dehydroascorbate reductase activity from Taiwanofungus camphorata (2009), J. Agric. Food Chem., 57, 10357-10362.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.8.5.1 | the coding region is expressed as a His-tagged fusion protein in Escherichia coli | Taiwanofungus camphoratus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.5.1 | 1.85 | - |
dehydroascorbate | - |
Taiwanofungus camphoratus |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.8.5.1 | 11000 | - |
calculated from cDNA | Taiwanofungus camphoratus |
| 1.8.5.1 | 15000 | - |
SDS-PAGE and electrospray ionization quadrupole-time-of-flight (ESI Q-TOF), His-tagged fusion protein | Taiwanofungus camphoratus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.8.5.1 | Taiwanofungus camphoratus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.8.5.1 | functional TcGrx is purified by Ni2+-nitrilotriacetic acid sepharose | Taiwanofungus camphoratus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.5.1 | glutathione + dehydroascorbate | - |
Taiwanofungus camphoratus | glutathione disulfide + ascorbate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.8.5.1 | monomer | electrospray ionization quadrupole-time-of-flight (ESI Q-TOF), 1 * 15000 Da (His-tagged fusion protein) | Taiwanofungus camphoratus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.8.5.1 | dehydroascorbate reductase | - |
Taiwanofungus camphoratus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.8.5.1 | 37 | - |
assay at | Taiwanofungus camphoratus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.8.5.1 | 100 | - |
the half-life of deactivation of the enzyme at 100°C is 8.5 min, and its thermal inactivation rate constant Kd is 0.0652/min | Taiwanofungus camphoratus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.5.1 | 21.51 | - |
dehydroascorbate | - |
Taiwanofungus camphoratus | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.8.5.1 | 7.4 | - |
assay at | Taiwanofungus camphoratus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.8.5.1 | 6 | 10 | - |
Taiwanofungus camphoratus |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.5.1 | 11.62 | - |
dehydroascorbate | - |
Taiwanofungus camphoratus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
