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Literature summary extracted from

  • Dutta, T.; Das, A.; Das, D.
    Purification and characterization of [Fe]-hydrogenase from high yielding hydrogen-producing strain, Enterobacter cloacae IIT-BT08 (MTCC 5373) (2009), Int. J. Hydrogen Energy, 34, 7530-7537.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.12.7.2 0.57
-
 reduced methyl viologen
-
 Enterobacter cloacae
1.12.7.2 0.72
-
 reduced ferredoxin
-
 Enterobacter cloacae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.12.7.2 cytoplasm
-
 Enterobacter cloacae 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.12.7.2 Fe contains ca. 11.47 gm-atom Fe/mol, iron molecules exist as [4Fe-4S] clusters Enterobacter cloacae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.12.7.2 50000
-
 gel filtration Enterobacter cloacae
1.12.7.2 50800
-
 1 * 50000, gel filtration and SDS-PAGE, 1 * 50800, mass spectrometry Enterobacter cloacae

Organism

EC Number Organism UniProt Comment Textmining
1.12.7.2 Enterobacter cloacae
-
 IIT-BT08 (MTCC 5373)
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.7.2 1284fold with a yield of 16.17%, by ultracentrifugation, ion exchange chromatography, gel filtration and hydrophobic-interaction chromatography Enterobacter cloacae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.12.7.2 0.26
-
 crude extract Enterobacter cloacae
1.12.7.2 335
-
 1284fold purified enzyme Enterobacter cloacae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.7.2 H+ + reduced benzyl viologen 58.8% relative activity, purified enzyme Enterobacter cloacae H2 + oxidized benzyl viologen
-
 ?
1.12.7.2 H+ + reduced ferredoxin 94.3% relative activity, purified enzyme Enterobacter cloacae H2 + oxidized ferredoxin
-
 ?
1.12.7.2 H+ + reduced methyl viologen 100% relative activity, purified enzyme Enterobacter cloacae H2 + oxidized methyl viologen
-
 ?
1.12.7.2 additional information no activity of the purified enzyme with methylene blue, NADH, NADPH and Na-dithionite as electron-donor Enterobacter cloacae ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.12.7.2 monomer 1 * 50000, gel filtration and SDS-PAGE, 1 * 50800, mass spectrometry Enterobacter cloacae

Synonyms

EC Number Synonyms Comment Organism
1.12.7.2 Fe-H2ase
-
 Enterobacter cloacae
1.12.7.2 Fe-hydrogenase
-
 Enterobacter cloacae
1.12.7.2 [Fe]-hydrogenase
-
 Enterobacter cloacae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.12.7.2 37
-
-
 Enterobacter cloacae

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.12.7.2 25 50 25°C: about 65% of maximal activity, 50: about 80% of maximal activity Enterobacter cloacae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.12.7.2 7 7.2
-
 Enterobacter cloacae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.12.7.2 6 8 pH 6.0: about 60% of maximal activity, pH 8.0: about 70% of maximal activity Enterobacter cloacae

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.12.7.2 Enterobacter cloacae isoelectric focusing
-
 5.6