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Literature summary extracted from

  • Mollania, N.; Khajeh, K.; Hosseinkhani, S.; Dabirmanesh, B.
    Purification and characterization of a thermostable phytate resistant alpha-amylase from Geobacillus sp. LH8 (2010), Int. J. Biol. Macromol., 46, 27-36.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.1 expressed in Escherichia coli BL21(DE3) cells Geobacillus sp.

General Stability

EC Number General Stability Organism
3.2.1.1 Ca2+ strongly stabilizes alpha-amylase while EDTA reduces its stability Geobacillus sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.1 Ba2+ 80% residual activity at 10 mM Geobacillus sp.
3.2.1.1 Co2+ 70% residual activity at 10 mM Geobacillus sp.
3.2.1.1 Cu2+ 86% residual activity at 10 mM Geobacillus sp.
3.2.1.1 EDTA 21% residual activity at 10 mM, alpha-amylase is completely inactivated after 6 min of incubation at 80°C in the presence of 5 mM EDTA Geobacillus sp.
3.2.1.1 Fe3+ 10% residual activity at 10 mM Geobacillus sp.
3.2.1.1 Mg2+ 40% residual activity at 10 mM Geobacillus sp.
3.2.1.1 additional information not inhibited by phytic acid Geobacillus sp.
3.2.1.1 Ni2+ 60% residual activity at 10 mM Geobacillus sp.
3.2.1.1 Zn2+ 64% residual activity at 10 mM Geobacillus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.1 additional information
-
 additional information the Km value for starch is 3 mg/ml at 65°C using potato starch (1% (w/v)) as a substrate in 20 mM Tris-HCl, pH 7.4 Geobacillus sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.1 Al3+ 120% relative activity at 10 mM Geobacillus sp.
3.2.1.1 Ca2+ 200% relative activity at 10 mM Geobacillus sp.
3.2.1.1 Cr3+ 140% relative activity at 10 mM Geobacillus sp.
3.2.1.1 K+ 105% relative activity at 10 mM Geobacillus sp.
3.2.1.1 Mn2+ 140% relative activity at 10 mM Geobacillus sp.
3.2.1.1 additional information not stimulated by Na+ Geobacillus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.1 52000
-
 SDS-PAGE Geobacillus sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.1 Geobacillus sp.
-
-
-
3.2.1.1 Geobacillus sp. LH8
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.1 ammonium sulfate precipitation, Q-Sepharose column chromatography, and Mono Q-Sepharose column chromatography Geobacillus sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.1 13.6
-
 after 13.6fold purification, at 65°C Geobacillus sp.
3.2.1.1 59
-
 crude extract, at 65°C Geobacillus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.1 potato starch + H2O
-
 Geobacillus sp. malto-oligosaccharides
-
 ?
3.2.1.1 potato starch + H2O
-
 Geobacillus sp. LH8 malto-oligosaccharides
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.1 1,4-alpha-D-glucan glucanohydrolase
-
 Geobacillus sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.1 70
-
 in the absence of Ca2+ Geobacillus sp.
3.2.1.1 80
-
 in the presence of 10 mM Ca2+ Geobacillus sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.1 85 90 29% activity is seen after 10 min at 85 and 90°C, respectively Geobacillus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.1 5 7
-
 Geobacillus sp.