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Literature summary extracted from

  • Carvalho, A.; Harduin-Lepers, A.; Magalhaes, A.; Machado, E.; Mendes, N.; Costa, L.; Matthiesen, R.; Almeida, R.; Costa, J.; Reis, C.
    Differential expression of alpha-2,3-sialyltransferases and alpha-1,3/4-fucosyltransferases regulates the levels of sialyl Lewis a and sialyl Lewis x in gastrointestinal carcinoma cells (2010), Int. J. Biochem. Cell Biol., 42, 80-89.
    View publication on PubMed

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.65 Homo sapiens
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Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.65 AGS cell
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 Homo sapiens
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2.4.1.65 AGS cell low expression of FUT1-6 and FUT9, highest expression of FUT10, FUT11 is absent Homo sapiens
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2.4.1.65 gastrointestinal cancer cell
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 Homo sapiens
-
2.4.1.65 GP-202 cell
-
 Homo sapiens
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2.4.1.65 HT-29 cell
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 Homo sapiens
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2.4.1.65 HT-29 cell very low expression of FUT1, FUT3, FUT6 and FUT9, low expression of FUT2, FUT5 and FUT10, higher expression of FUT4, highest expression of FUT11 Homo sapiens
-
2.4.1.65 IPA-220 cell
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 Homo sapiens
-
2.4.1.65 KATO-III cell
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 Homo sapiens
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2.4.1.65 KATO-III cell very low expression of FUT1, low expression of FUT2, higher expression of FUT4-6, highest expression of FUT3 and FUT10-11 Homo sapiens
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2.4.1.65 MKN-45 cell
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 Homo sapiens
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2.4.1.65 MKN-45 cell very low expression of FUT1, FUT5 and FUT6, low expression of FUT2, higher expression of FUT9-11, FUT3 is absent, highest expression of FUT4 Homo sapiens
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2.4.1.65 additional information low expression of FUT1-6 and FUT9 in GP-202 cells, but highest expression of FUT10 and FUT11. Very low expression of FUT1 in IPA-220 cells, low expression of FUT2, higher expression of FUT3-4, FUT6 and FUT10-11, highest expression of FUT5 and FUT9 Homo sapiens
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.65 Fucalpha(1-2)Galbeta(1-3)GlcNAcbeta(1-)(CH2)3-NHCO-(CH2)5-NH-biotin + GDP-L-fucose
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 Homo sapiens Fucalpha(1-2)Galbeta(1-3)[Fucalpha(1-4)]GlcNAcbeta(1-)(CH2)3-NHCO-(CH2)5-NH-biotin + GDP
-
 ?
2.4.1.65 Fucalpha(1-2)Galbeta(1-4)GlcNAcbeta(1-)(CH2)3-NHCO-(CH2)5-NH-biotin + GDP-L-fucose
-
 Homo sapiens Fucalpha(1-2)Galbeta(1-4)[Fucalpha(1-3)]GlcNAcbeta(1-)(CH2)3-NHCO-(CH2)5-NH-biotin + GDP
-
 ?
2.4.1.65 Galbeta(1-3)GlcNAcbeta(1-)(CH2)3-NHCO-(CH2)5-NH-biotin + GDP-L-fucose
-
 Homo sapiens Galbeta(1-3)[Fucalpha(1-4)]GlcNAcbeta(1-)(CH2)3-NHCO-(CH2)5-NH-biotin + GDP
-
 ?
2.4.1.65 Galbeta(1-4)GlcNAcbeta(1-)(CH2)3-NHCO-(CH2)5-NH-biotin + GDP-L-fucose
-
 Homo sapiens Galbeta(1-4)[Fucalpha(1-3)]GlcNAcbeta(1-)(CH2)3-NHCO-(CH2)5-NH-biotin + GDP
-
 ?
2.4.1.65 GDP-beta-L-fucose + Fucalpha(1->2)Galbeta(1->3)GlcNAc-(CH2)3-NHCO-(CH2)5-NH-biotin
-
 Homo sapiens ?
-
 ?
2.4.1.65 GDP-beta-L-fucose + Fucalpha1,2Galbeta(1->4)GlcNAc-(CH2)3-NHCO-(CH2)5-NH-biotin
-
 Homo sapiens ?
-
 ?
2.4.1.65 GDP-beta-L-fucose + Galbeta(1->3)GlcNAc-(CH2)3-NHCO-(CH2)5-NH-biotin
-
 Homo sapiens ?
-
 ?
2.4.1.65 GDP-beta-L-fucose + Galbeta(1->4)GlcNAc-(CH2)3-NHCO-(CH2)5-NH-biotin
-
 Homo sapiens ?
-
 ?
2.4.1.65 GDP-beta-L-fucose + NeuAcalpha(2->3)Galbeta(1->3)GlcNAc-(CH2)3-NHCO-(CH2)5-NH-biotin
-
 Homo sapiens ?
-
 ?
2.4.1.65 GDP-beta-L-fucose + NeuAcalpha(2->3)Galbeta(1->4)GlcNAc-(CH2)3-NHCO-(CH2)5-NH-biotin
-
 Homo sapiens ?
-
 ?
2.4.1.65 additional information FUT6 can synthesize sialyl Lewis x in KATO III cells whereas FUT5 is most likely responsible for the synthesis of this epitope in HT29 and MKN45 cells. FUT3 and to a lower extent FUT5 have the capability to synthesize sialyl Lewis a Homo sapiens ?
-
 ?
2.4.1.65 additional information the enzyme transfers fucose from the GDP-fucose donor onto different glycoconjugate acceptors, such as, glycoproteins, glycolipids and oligosaccharides. Isoforms FUT3 to FUT7 and FUT9 to FUT11 belong to the group of alpha1,3/4-fucosyltransferases. FUT3 predominantly exhibits alpha1,4-fucosyltransferase activity synthesizing Lewis a, NeuAcalpha2,3Galbeta(1->3)[Fucalpha(1->4)]GlcNAc-R and Lewis b, and a minor alpha1,3-FUT activity, synthesizing Lewis x, sialyl Lewis x and Lewis y. FUT5 and FUT6 synthesize Lewis x and NeuAcalpha2,3Galbeta(1->4)[Fucalpha(1->3)]GlcNAc-R. FUT7 is an alpha1,3-FUT that synthesizes only sialyl Lewis x. FUT4 and FUT9 produce Lewis x and Lewis y. FUT10 and FUt11 exhibit alpha1,3-fucosyltransferase activity Homo sapiens ?
-
 ?
2.4.1.65 NeuAcalpha(2-3)Galbeta(1-3)GlcNAcbeta(1-)(CH2)3-NHCO-(CH2)5-NH-biotin + GDP-L-fucose
-
 Homo sapiens NeuAcalpha(2-3)Galbeta(1-3)[Fucalpha(1-4)]GlcNAcbeta(1-)(CH2)3-NHCO-(CH2)5-NH-biotin + GDP
-
 ?
2.4.1.65 NeuAcalpha(2-3)Galbeta(1-4)GlcNAcbeta(1-)(CH2)3-NHCO-(CH2)5-NH-biotin + GDP-L-fucose
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 Homo sapiens NeuAcalpha(2-3)Galbeta(1-4)[Fucalpha(1-3)]GlcNAcbeta(1-)(CH2)3-NHCO-(CH2)5-NH-biotin + GDP
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.65 alpha-1,3/4-fucosyltransferase
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 Homo sapiens
2.4.1.65 alpha1,3/4-fucosyltransferase
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 Homo sapiens
2.4.1.65 alpha1,3/4-FUT
-
 Homo sapiens
2.4.1.65 FucT III isoform Homo sapiens
2.4.1.65 FucT V isoform Homo sapiens
2.4.1.65 FucT VI isoform Homo sapiens
2.4.1.65 FUT10
-
 Homo sapiens
2.4.1.65 FUT11
-
 Homo sapiens
2.4.1.65 FUT3
-
 Homo sapiens
2.4.1.65 FUT3 isoform Homo sapiens
2.4.1.65 FUT4
-
 Homo sapiens
2.4.1.65 FUT5
-
 Homo sapiens
2.4.1.65 FUT5 isoform Homo sapiens
2.4.1.65 FUT6
-
 Homo sapiens
2.4.1.65 FUT6 isoform Homo sapiens
2.4.1.65 FUT7
-
 Homo sapiens
2.4.1.65 Fut9
-
 Homo sapiens

Expression

EC Number Organism Comment Expression
2.4.1.65 Homo sapiens decrease in FUT4 transcript levels in MKN45 confluent cells down
2.4.1.65 Homo sapiens increase in mRNA levels of FUT5 and FUT6 in MKN45 confluent cells up

General Information

EC Number General Information Comment Organism
2.4.1.65 physiological function final glycosylation step in sialyl Lewis x and sialyl Lewis a biosynthesis in MKN45 cell line is associated to FUT5, which efficiently fucosylates sialyl Lewis precursors on glycoproteins Homo sapiens
2.4.1.65 physiological function fucosyltransferases are responsible for the synthesis of sialyl Lewis a and sialyl Lewis x in gastrointestinal cell lines Homo sapiens