BRENDA - Enzyme Database

Aphidius ervi teratocytes release an extracellular enolase

Falabella, P.; Riviello, L.; De Stradis, M.L.; Stigliano, C.; Varricchio, P.; Grimaldi, A.; de Eguileor, M.; Graziani, F.; Gigliotti, S.; Pennacchio, F.; Insect Biochem. Mol. Biol. 39, 801-813 (2009)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.2.1.11
expressed in Escherichia coli Rosettagami (DE3) cells
Aphidius ervi
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.11
0.086
-
2-phospho-D-glycerate
in 81 mM triethanolamine/HCl, pH 7.4, at 25°C
Aphidius ervi
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.2.1.11
extracellular
-
Aphidius ervi
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.11
Mg2+
the highest activation rate of the enzymatic activity is reached at 5 mM Mg2+ and remains unchanged up to 25 mM
Aphidius ervi
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
49000
-
2 * 49000, gel filtration, the recombinant protein produced in bacteria under native conditions is a dimer
Aphidius ervi
4.2.1.11
98000
-
gel filtration
Aphidius ervi
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.11
Aphidius ervi
C9YSH7
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.11
Ni-NTA-agarose column chromatography, gel filtration
Aphidius ervi
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.11
embryo
-
Aphidius ervi
-
4.2.1.11
larva
-
Aphidius ervi
-
4.2.1.11
teratocyte
present in the teratocytes and released in the host haemocoel
Aphidius ervi
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
704025
Aphidius ervi
phosphoenolpyruvate + H2O
-
-
-
r
Subunits
EC Number
Subunits
Commentary
Organism
4.2.1.11
dimer
2 * 49000, gel filtration, the recombinant protein produced in bacteria under native conditions is a dimer
Aphidius ervi
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
expressed in Escherichia coli Rosettagami (DE3) cells
Aphidius ervi
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.11
0.086
-
2-phospho-D-glycerate
in 81 mM triethanolamine/HCl, pH 7.4, at 25°C
Aphidius ervi
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.2.1.11
extracellular
-
Aphidius ervi
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.11
Mg2+
the highest activation rate of the enzymatic activity is reached at 5 mM Mg2+ and remains unchanged up to 25 mM
Aphidius ervi
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
49000
-
2 * 49000, gel filtration, the recombinant protein produced in bacteria under native conditions is a dimer
Aphidius ervi
4.2.1.11
98000
-
gel filtration
Aphidius ervi
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
Ni-NTA-agarose column chromatography, gel filtration
Aphidius ervi
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.11
embryo
-
Aphidius ervi
-
4.2.1.11
larva
-
Aphidius ervi
-
4.2.1.11
teratocyte
present in the teratocytes and released in the host haemocoel
Aphidius ervi
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
704025
Aphidius ervi
phosphoenolpyruvate + H2O
-
-
-
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.2.1.11
dimer
2 * 49000, gel filtration, the recombinant protein produced in bacteria under native conditions is a dimer
Aphidius ervi