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Literature summary extracted from

  • Falabella, P.; Riviello, L.; De Stradis, M.L.; Stigliano, C.; Varricchio, P.; Grimaldi, A.; de Eguileor, M.; Graziani, F.; Gigliotti, S.; Pennacchio, F.
    Aphidius ervi teratocytes release an extracellular enolase (2009), Insect Biochem. Mol. Biol., 39, 801-813.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.11 expressed in Escherichia coli Rosettagami (DE3) cells Aphidius ervi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.11 0.086
-
 2-phospho-D-glycerate in 81 mM triethanolamine/HCl, pH 7.4, at 25°C Aphidius ervi

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.1.11 extracellular
-
 Aphidius ervi
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.11 Mg2+ the highest activation rate of the enzymatic activity is reached at 5 mM Mg2+ and remains unchanged up to 25 mM Aphidius ervi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.11 49000
-
 2 * 49000, gel filtration, the recombinant protein produced in bacteria under native conditions is a dimer Aphidius ervi
4.2.1.11 98000
-
 gel filtration Aphidius ervi

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.11 Aphidius ervi C9YSH7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.11 Ni-NTA-agarose column chromatography, gel filtration Aphidius ervi

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.1.11 embryo
-
 Aphidius ervi
-
4.2.1.11 larva
-
 Aphidius ervi
-
4.2.1.11 teratocyte present in the teratocytes and released in the host haemocoel Aphidius ervi
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.11 2-phospho-D-glycerate
-
 Aphidius ervi phosphoenolpyruvate + H2O
-
 r

Subunits

EC Number Subunits Comment Organism
4.2.1.11 dimer 2 * 49000, gel filtration, the recombinant protein produced in bacteria under native conditions is a dimer Aphidius ervi

Synonyms

EC Number Synonyms Comment Organism
4.2.1.11 ENO
-
 Aphidius ervi
4.2.1.11 enolase
-
 Aphidius ervi
4.2.1.11 p45
-
 Aphidius ervi