BRENDA - Enzyme Database

Identification and characterization of two arginine kinases from the parasitic insect Ctenocephalides felis

Werr, M.; Cramer, J.; Ilg, T.; Insect Biochem. Mol. Biol. 39, 634-645 (2009)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.3.3
0.21
-
L-Arg
isoform arginine kinase 2
Ctenocephalides felis
2.7.3.3
0.284
-
ADP
isoform arginine kinase 2
Ctenocephalides felis
2.7.3.3
0.7
-
ADP
isoform arginine kinase 1
Ctenocephalides felis
2.7.3.3
0.74
-
Nomega-phospho-L-Arg
isoform arginine kinase 2
Ctenocephalides felis
2.7.3.3
1.27
-
L-Arg
isoform arginine kinase 1
Ctenocephalides felis
2.7.3.3
2.31
-
Nomega-phospho-L-Arg
isoform arginine kinase 1
Ctenocephalides felis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.3.3
41500
-
isoform AK1, estimated from SDS-PAGE
Ctenocephalides felis
2.7.3.3
43400
-
isoform AK2, estimated from SDS-PAGE
Ctenocephalides felis
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.3.3
Ctenocephalides felis
-
cat flea
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.3.3
Ni2+-NTA agarose column chromatography
Ctenocephalides felis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.3.3
ATP + L-Arg
highest activity
704021
Ctenocephalides felis
ADP + Nomega-phospho-L-Arg
-
-
-
?
2.7.3.3
ATP + L-arginine-O-ethyl ester
isoform arginine kinase 2 shows about 16% activity of that obtained with L-Arg
704021
Ctenocephalides felis
?
-
-
-
?
2.7.3.3
ATP + L-canavanine
isoform arginine kinase 1 shows 10% activity of that obtained with L-Arg, isoform arginine kinase 2 shows about 9.5% activity of that obtained with L-Arg
704021
Ctenocephalides felis
ADP + L-phosphocanavanine
-
-
-
?
2.7.3.3
ATP + L-homoarginine
isoform arginine kinase 2 shows about 37% activity of that obtained with L-Arg
704021
Ctenocephalides felis
ADP + Nomega-phospho-L-homoarginine
-
-
-
?
2.7.3.3
additional information
L-arginine-O-ethyl ester and L-homo-arginine are extremely poor substrates for isoform AK1 with only 3.5% and 2% of the L-arginine reaction rate. L-Nalpha-acetyl-arginine, agmatine, creatine, 4-guanidino butyric acid, N7-methyl-arginine and N7-nitro-arginine are no substrates for isoform AK1. Minor substrates for isoform AK2 are L-Nalpha-acetyl-arginine and 4-guanidino butyric acid with 1.5% and 1.9% of the L-arginine reaction rate, respectively. Agmatine, creatine, L-N7-methyl-arginine and L-N7-nitro-arginine are no substrates for isoform AK2
704021
Ctenocephalides felis
?
-
-
-
-
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.7.3.3
ATP
-
Ctenocephalides felis
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.7.3.3
ATP
-
Ctenocephalides felis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.3.3
0.21
-
L-Arg
isoform arginine kinase 2
Ctenocephalides felis
2.7.3.3
0.284
-
ADP
isoform arginine kinase 2
Ctenocephalides felis
2.7.3.3
0.7
-
ADP
isoform arginine kinase 1
Ctenocephalides felis
2.7.3.3
0.74
-
Nomega-phospho-L-Arg
isoform arginine kinase 2
Ctenocephalides felis
2.7.3.3
1.27
-
L-Arg
isoform arginine kinase 1
Ctenocephalides felis
2.7.3.3
2.31
-
Nomega-phospho-L-Arg
isoform arginine kinase 1
Ctenocephalides felis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.3.3
41500
-
isoform AK1, estimated from SDS-PAGE
Ctenocephalides felis
2.7.3.3
43400
-
isoform AK2, estimated from SDS-PAGE
Ctenocephalides felis
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.3.3
Ni2+-NTA agarose column chromatography
Ctenocephalides felis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.3.3
ATP + L-Arg
highest activity
704021
Ctenocephalides felis
ADP + Nomega-phospho-L-Arg
-
-
-
?
2.7.3.3
ATP + L-arginine-O-ethyl ester
isoform arginine kinase 2 shows about 16% activity of that obtained with L-Arg
704021
Ctenocephalides felis
?
-
-
-
?
2.7.3.3
ATP + L-canavanine
isoform arginine kinase 1 shows 10% activity of that obtained with L-Arg, isoform arginine kinase 2 shows about 9.5% activity of that obtained with L-Arg
704021
Ctenocephalides felis
ADP + L-phosphocanavanine
-
-
-
?
2.7.3.3
ATP + L-homoarginine
isoform arginine kinase 2 shows about 37% activity of that obtained with L-Arg
704021
Ctenocephalides felis
ADP + Nomega-phospho-L-homoarginine
-
-
-
?
2.7.3.3
additional information
L-arginine-O-ethyl ester and L-homo-arginine are extremely poor substrates for isoform AK1 with only 3.5% and 2% of the L-arginine reaction rate. L-Nalpha-acetyl-arginine, agmatine, creatine, 4-guanidino butyric acid, N7-methyl-arginine and N7-nitro-arginine are no substrates for isoform AK1. Minor substrates for isoform AK2 are L-Nalpha-acetyl-arginine and 4-guanidino butyric acid with 1.5% and 1.9% of the L-arginine reaction rate, respectively. Agmatine, creatine, L-N7-methyl-arginine and L-N7-nitro-arginine are no substrates for isoform AK2
704021
Ctenocephalides felis
?
-
-
-
-