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Literature summary extracted from

  • Terra, V.S.; Homer, K.A.; Rao, S.G.; Andrew, P.W.; Yesilkaya, H.
    Characterization of novel beta-galactosidase activity that contributes to glycoprotein degradation and virulence in Streptococcus pneumoniae (2010), Infect. Immun., 78, 348-357.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.23 expressed in Escherichia coli BL21(DE3) cells Streptococcus pneumoniae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.23 0.91
-
 4-nitrophenyl-beta-D-galactopyranoside at pH 6.5 Streptococcus pneumoniae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.23 69000
-
 SDS-PAGE Streptococcus pneumoniae

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.23 Streptococcus pneumoniae
-
 serotype 2 strain D39
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.23 glycoprotein
-
 Streptococcus pneumoniae

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.23 Imac resin column chromatography Streptococcus pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.23 4-nitrophenyl beta-D-galactopyranoside + H2O
-
 Streptococcus pneumoniae 4-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 additional information the SPD0065 protein shows no activity towards 4-methylumbelliferyl-N-acetyl-alpha-D-glucosaminide, 4-methylumbeliferyl-alpha-D-glucopyranoside, 2-nitrophenyl-alpha-D-galactopyranoside, 4-nitrophenyl-alpha-D-glucoside 6-phosphate, 4-nitrophenyl-alpha-D-mannose 6-phosphate, 4-nitrophenyl-alpha-D-galactose 6-phosphate, and 4-nitrophenyl-beta-D-glucose 6-phosphate Streptococcus pneumoniae ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.23 SPD_0065 protein
-
 Streptococcus pneumoniae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.23 6.5
-
-
 Streptococcus pneumoniae

Expression

EC Number Organism Comment Expression
3.2.1.23 Streptococcus pneumoniae the expression of SPD_0065 protein in cells grown in mucin-containing medium or tissues harvested from infected animals is significantly upregulated compared to that in pneumococci from glucose-containing medium up