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Literature summary extracted from

  • Yesilkaya, H.; Spissu, F.; Carvalho, S.M.; Terra, V.S.; Homer, K.A.; Benisty, R.; Porat, N.; Neves, A.R.; Andrew, P.W.
    Pyruvate formate lyase is required for pneumococcal fermentative metabolism and virulence (2009), Infect. Immun., 77, 5418-5427.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.54 PCR-amplificatioin, mariner plasmid Streptococcus pneumoniae D39

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.54 additional information no formate production and decrease of unsaturation index in downstream (in absence of acetate) fatty acid production in mutants SPD0420M (enzyme mutant) and SPD1774M (activating enzyme mutant), restored by complementation, increased survival and lower disease sign score of mice infected with mutant strains, wild-type survival rate in complemented mutants, lower nasopharynx numbers and lower survival in lungs, intravenous infection with similar growth of mutant and wild-type. All mutant and wild-type strains grow better under aerobic than anaerobic conditions with glucose as sole carbon source, no difference in growth rate and yields between mutants and wild-type, with galactose (generally lower growth rate than with glucose) as carbon source all but strain SPD1774M grow better under aerobic conditions, strain SPD1774M better under anaerobic conditions, strain SPD0420M has lower growth rate than wild-type under anaerobiosis, the complemented strain SPD0420Comp grows better than SPD0420M in anaerobiosis. Mutants SPD035M and SPD0229M do not affect pyruvate formate lyase dependent product formation. Streptococcus pneumoniae D39

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.54 oxamate isosteric, chemically inert pyruvate analogue Streptococcus pneumoniae D39

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.54 pyruvate + CoA Streptococcus pneumoniae D39
-
 formate + acetyl-CoA
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.54 Streptococcus pneumoniae D39
-
 slightly aerobic and anaerobic growth, and intraperitoneal infection or nasal infection of female MFI mice
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.3.1.54 additional information PFL-activating enzyme Streptococcus pneumoniae D39

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.54 pyruvate + CoA
-
 Streptococcus pneumoniae D39 formate + acetyl-CoA
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.3.1.54 PFL
-
 Streptococcus pneumoniae D39
2.3.1.54 pyruvate formate lyase
-
 Streptococcus pneumoniae D39

Expression

EC Number Organism Comment Expression
2.3.1.54 Streptococcus pneumoniae D39 9fold increase in SPD0420 mutants in galactose compared to glucose-grown bacteria in anaerobic conditions, in aerobiosis, 2.4fold upregulation of SpD0420 mutant, 3.5fold in SPD1774 mutant in galactose compared to glucose-grown bacteria up

General Information

EC Number General Information Comment Organism
2.3.1.54 metabolism anaerobic glucose metabolism, direct link between fermentative metabolism and virulence in infected mice Streptococcus pneumoniae D39