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Literature summary extracted from

  • Hsu, W.H.; Ong, P.L.; Chen, S.C.; Lin, L.L.
    Contribution of Ser463 residue to the enzymatic and autoprocessing activities of Escherichia coli gamma-glutamyltranspeptidase (2009), Indian J. Biochem. Biophys., 46, 281-288.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.3.2.2 S463D complete loss of activity, impaired autoproteolytic processing, increase in the critical bond distance of residues Q390-T391 Escherichia coli
2.3.2.2 S463K complete loss of activity, impaired autoproteolytic processing, increase in the critical bond distance of residues Q390-T391 Escherichia coli
2.3.2.2 S463T 40% decrease in ratio kcat/KM Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.2.2 0.045
-
 5-L-glutamyl-4-nitroanilide wild-type, pH 9.0, 37°C Escherichia coli
2.3.2.2 0.058
-
 5-L-glutamyl-4-nitroanilide mutant S463T, pH 9.0, 37°C Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.2 Escherichia coli P18956
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.2.2 4.1
-
 mutant S463T, pH 9.0, 37°C Escherichia coli
2.3.2.2 6.8
-
 wild-type, pH 9.0, 37°C Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.2 5-L-glutamyl-4-nitroanilide + Gly-Gly
-
 Escherichia coli 4-nitroaniline + 5-L-glutamyl-Gly-Gly
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.2.2 0.49
-
 5-L-glutamyl-4-nitroanilide mutant S463T, pH 9.0, 37°C Escherichia coli
2.3.2.2 0.62
-
 5-L-glutamyl-4-nitroanilide wild-type, pH 9.0, 37°C Escherichia coli

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.2.2 0.008
-
 5-L-glutamyl-4-nitroanilide mutant S463T, pH 9.0, 37°C Escherichia coli
2.3.2.2 0.014
-
 5-L-glutamyl-4-nitroanilide wild-type, pH 9.0, 37°C Escherichia coli