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Literature summary extracted from

  • Yao, D.; Mizuguchi, H.; Yamaguchi, M.; Yamada, H.; Chida, J.; Shikata, K.; Kido, H.
    Thermal instability of compound variants of carnitine palmitoyltransferase II and impaired mitochondrial fuel utilization in influenza-associated encephalopathy (2008), Hum. Mutat., 29, 718-727.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.21 expressed in COS-7 cell Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.21 F352C naturally occuring mutation Homo sapiens
2.3.1.21 F352C/V368I double mutant with naturally occuring mutations Homo sapiens
2.3.1.21 F352C/V368I/V605L triple mutant with naturally occuring mutations Homo sapiens
2.3.1.21 M647V naturally occuring mutation Homo sapiens
2.3.1.21 P504L naturally occuring mutation Homo sapiens
2.3.1.21 P504L/V605L double mutant with naturally occuring mutations Homo sapiens
2.3.1.21 V368I naturally occuring mutation Homo sapiens
2.3.1.21 V605L naturally occuring mutation Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.21 0.12
-
 L-carnitine wild-type protein, COS-7 cell lysate Homo sapiens
2.3.1.21 0.13
-
 L-carnitine V368I mutant protein, COS-7 cell lysate Homo sapiens
2.3.1.21 0.14
-
 L-carnitine M647V mutant protein, COS-7 cell lysate Homo sapiens
2.3.1.21 0.35
-
 L-carnitine P504L mutant protein, COS-7 cell lysate Homo sapiens
2.3.1.21 0.35
-
 L-carnitine V605L mutant protein, COS-7 cell lysate Homo sapiens
2.3.1.21 0.36
-
 L-carnitine F352C mutant protein, COS-7 cell lysate Homo sapiens
2.3.1.21 0.37
-
 L-carnitine F352C/V368I mutant protein, COS-7 cell lysate Homo sapiens
2.3.1.21 0.37
-
 L-carnitine wild-type protein and P504L/V605L mutant protein, COS-7 cell lysate (doubly transfected) Homo sapiens
2.3.1.21 0.38
-
 L-carnitine P504L/V605L mutant protein, COS-7 cell lysate Homo sapiens
2.3.1.21 0.52
-
 L-carnitine F352C/V368I/V605L mutant protein, COS-7 cell lysate Homo sapiens
2.3.1.21 0.53
-
 L-carnitine wild-type protein and F352C/V368I/V605L mutant protein, COS-7 cell lysate (doubly transfected) Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.21 mitochondrial inner membrane
-
 Homo sapiens 5743
-

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.21 Homo sapiens P23786
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.21 0.00062
-
 F352C/V368I/V605L mutant protein, COS-7 cell lysate Homo sapiens
2.3.1.21 0.00063
-
 wild-type protein and F352C/V368I/V605L mutant protein, COS-7 cell lysate (doubly transfected) Homo sapiens
2.3.1.21 0.00071
-
 P504L/V605L mutant protein, COS-7 cell lysate Homo sapiens
2.3.1.21 0.00072
-
 F352C/V368I mutant protein, COS-7 cell lysate Homo sapiens
2.3.1.21 0.00072
-
 wild-type protein and P504L/V605L mutant protein, COS-7 cell lysate (doubly transfected) Homo sapiens
2.3.1.21 0.00073
-
 F352C mutant protein, COS-7 cell lysate Homo sapiens
2.3.1.21 0.00076
-
 P504L mutant protein, COS-7 cell lysate Homo sapiens
2.3.1.21 0.00078
-
 V605L mutant protein, COS-7 cell lysate Homo sapiens
2.3.1.21 0.00081
-
 M647V mutant protein, COS-7 cell lysate Homo sapiens
2.3.1.21 0.00085
-
 V368I mutant protein, COS-7 cell lysate Homo sapiens
2.3.1.21 0.00087
-
 wild-type protein, COS-7 cell lysate Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.21 L-carnitine + palmitoyl-CoA
-
 Homo sapiens L-palmitoylcarnitine + CoA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.21 carnitine palmitoyltransferase II
-
 Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.21 additional information
-
 mutant proteins V605L, F352C, and P504L: enzyme activities of transfected cells are significantly reduced to 59.5 to 43.6% during incubation for 2 hours at 41°C, mutant protein F352C/V368I/V605L: enzyme activity of transfected cells is reduced to 19.4% during incubation for 2 hours at 41°C Homo sapiens