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Literature summary extracted from

  • Baksi, K.; Zhang, Z.; Banerjee, A.; Banerjee, D.K.
    Cloning and expression of mannosylphospho dolichol synthase from bovine adrenal medullary capillary endothelial cells (2009), Glycoconj. J., 26, 635-645.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.83 DPMS (KB-3) gene cloned into the pcDNA3.1/His A vector at the KpnI and XhoI site and transformed into DH5alpha cells. Cloned into Xho I and BamH I sites of pEGFP-N1 to obtain pEGFP-N1-DPMS overexpression plasmid. Transfection of capillary endothelial cells with pEGFP-N1-DPMS overexpression plasmid and pEGFP-N1 vector Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.83 membrane DPMS has one membrane spanning region Bos taurus 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.83 33000
-
 x * 33000, recombinant His-tag DPMS, SDS-PAGE Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.83 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.83 His-tag fusion protein purified using ProBond purification system, more than 97% pure Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.83 adrenal medulla capillary endothelial cell Bos taurus
-

Subunits

EC Number Subunits Comment Organism
2.4.1.83 ? x * 33000, recombinant His-tag DPMS, SDS-PAGE Bos taurus

Synonyms

EC Number Synonyms Comment Organism
2.4.1.83 DPMS
-
 Bos taurus
2.4.1.83 mannosylphospho dolichol synthase
-
 Bos taurus

Expression

EC Number Organism Comment Expression
2.4.1.83 Bos taurus capillary endothelial cells harboring DPMS overexpressing plasmid express DPMS higher than pEGFP-N1 vector and the parental cells up

General Information

EC Number General Information Comment Organism
2.4.1.83 physiological function overexpression of DPMS accelerates angiogenesis, a higher rate of cellular proliferation in DPMS overexpressing cells compared to the pEGFP-N1 vector and the parental cells Bos taurus