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Literature summary extracted from

  • Sugiura, N.; Shimokata, S.; Minamisawa, T.; Hirabayashi, J.; Kimata, K.; Watanabe, H.
    Sequential synthesis of chondroitin oligosaccharides by immobilized chondroitin polymerase mutants (2008), Glycoconj. J., 25, 521-530.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.175 expressed in Escherichia Top10 cells Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.175 D241K inactive using UDP-N-acetyl-beta-D-galactosamine as substrate Escherichia coli
2.4.1.175 D521K inactive using UDP-N-glucuronic acid as substrate Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.175 82000
-
 SDS-PAGE Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.175 Escherichia coli
-
-
-
2.4.1.175 Escherichia coli K4
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.175 Ni-NTA agarose column chromatography Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.175 UDP-alpha-D-glucuronate + chondroitin hexasaccharide
-
 Escherichia coli UDP + ?
-
 ?
2.4.1.175 UDP-alpha-D-glucuronate + chondroitin hexasaccharide
-
 Escherichia coli K4 UDP + ?
-
 ?
2.4.1.175 UDP-alpha-D-glucuronate + N-acetyl-beta-D-galactosaminyl-[GlcA-GalNAc]n-pyridylamine
-
 Escherichia coli UDP + [GlcA-GalNAc]n+1-pyridylamine
-
 ?
2.4.1.175 UDP-N-acetyl-alpha-D-galactosamine + chondroitin hexasaccharide
-
 Escherichia coli UDP + N-acetyl-beta-D-galactosaminyl chondroitin hexasaccharide
-
 ?
2.4.1.175 UDP-N-acetyl-alpha-D-galactosamine + chondroitin hexasaccharide
-
 Escherichia coli K4 UDP + N-acetyl-beta-D-galactosaminyl chondroitin hexasaccharide
-
 ?
2.4.1.175 UDP-N-acetyl-alpha-D-galactosamine + chondroitin pentasaccharide
-
 Escherichia coli UDP + N-acetyl-beta-D-galactosaminyl chondroitin pentasaccharide
-
 ?
2.4.1.175 UDP-N-acetyl-alpha-D-galactosamine + chondroitin pentasaccharide
-
 Escherichia coli K4 UDP + N-acetyl-beta-D-galactosaminyl chondroitin pentasaccharide
-
 ?
2.4.1.175 UDP-N-acetyl-alpha-D-galactosamine + chondroitin polysaccharide
-
 Escherichia coli UDP + N-acetyl-beta-D-galactosaminyl chondroitin polysaccharide
-
 ?
2.4.1.175 UDP-N-acetyl-alpha-D-galactosamine + chondroitin polysaccharide
-
 Escherichia coli K4 UDP + N-acetyl-beta-D-galactosaminyl chondroitin polysaccharide
-
 ?
2.4.1.175 UDP-N-acetyl-alpha-D-galactosamine + [GlcA-GalNAc]n-pyridylamine
-
 Escherichia coli UDP + N-acetyl-beta-D-galactosaminyl-[GlcA-GalNAc]n-pyridylamine
-
 ?
2.4.1.175 UDP-N-acetyl-alpha-D-galactosamine + [GlcA-GalNAc]n-pyridylamine
-
 Escherichia coli K4 UDP + N-acetyl-beta-D-galactosaminyl-[GlcA-GalNAc]n-pyridylamine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.175 chondroitin polymerase
-
 Escherichia coli
2.4.1.175 K4CP
-
 Escherichia coli