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Literature summary extracted from

  • Sindhuwinata, N.; Munoz, E.; Munoz, F.J.; Palcic, M.M.; Peters, H.; Peters, T.
    Binding of an acceptor substrate analog enhances the enzymatic activity of human blood group B galactosyltransferase (2010), Glycobiology, 20, 718-723.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.37 alpha-L-Fuc-(1,2)-beta-D-3-deoxy-Gal-O-octyl i.e. 3DD, an acceptor substrate analogue, accelerates enzymatic hydrolysis of UDP-Gal Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.37 overexpression in Escherichia coli strain BL21 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.37 Mg2+ activates, GTB requires divalent metal ions for full activity Homo sapiens
2.4.1.37 Mn2+ activates 9fold at 24°C, GTB requires divalent metal ions for full activity Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.37 UDP-galactose + alpha-L-fucosyl-(1,2)-D-galactose Homo sapiens i.e. H disaccharide UDP + alpha-D-galactosyl-(1,3)-[alpha-L-fucosyl(1,2)]-D-galactose
-
?
2.4.1.37 UDP-galactose + blood group antigen Homo sapiens GTB catalyzes the transfer of galactose from UDP-Gal to the C3 position of the terminal galactose of H antigen acceptors UDP + alpha-D-galactosyl-blood group antigen
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.37 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.37 recombinant enzyme from Escherichia coli by cation exchange chromatograophy, UDP-hexanolamine affinity chromatography, and dialysis Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.37 UDP-galactose + alpha-L-fucosyl-(1,2)-D-galactose i.e. H disaccharide Homo sapiens UDP + alpha-D-galactosyl-(1,3)-[alpha-L-fucosyl(1,2)]-D-galactose
-
?
2.4.1.37 UDP-galactose + blood group antigen GTB catalyzes the transfer of galactose from UDP-Gal to the C3 position of the terminal galactose of H antigen acceptors Homo sapiens UDP + alpha-D-galactosyl-blood group antigen
-
?
2.4.1.37 UDP-galactose + blood group antigen H GTB catalyzes the transfer of galactose from UDP-Gal to the C3 position of the terminal galactose of H antigen acceptors Homo sapiens UDP + alpha-D-galactosyl-blood group antigen H
-
?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.37 blood group B galactosyltransferase
-
Homo sapiens
2.4.1.37 GTB
-
Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.37 37
-
assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.37 6.5
-
-
Homo sapiens

General Information

EC Number General Information Comment Organism
2.4.1.37 physiological function human blood group B galactosyltransferase responsible for the biosynthesis of human blood group antigens Homo sapiens