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Literature summary extracted from

  • Prinetti, A.; Aureli, M.; Illuzzi, G.; Prioni, S.; Nocco, V.; Scandroglio, F.; Gagliano, N.; Tredici, G.; Rodriguez-Menendez, V.; Chigorno, V.; Sonnino, S.
    GM3 synthase overexpression results in reduced cell motility and in caveolin-1 upregulation in human ovarian carcinoma cells (2010), Glycobiology, 20, 62-77.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.3.9 SAT-1 cDNA cloned into plasmid expression vector pRc/CMV under control of CMV promoter, overexpression in A2780 ovarian tumor cells Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.3.9 additional information motility of low GM3 synthase expressing cells is reduced in the presence of a Src kinase inhibitor Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.92 Homo sapiens
-
-
-
2.4.3.9 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.92 A-2780 cell
-
 Homo sapiens
-
2.4.1.92 ovary cancer cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.3.9 CMP-Neu5Ac + lactosylceramide caveolin-1 is a GM3-interacting protein in GM3 synthase overexpressing cells Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.92 GM3 synthase
-
 Homo sapiens
2.4.3.9 GM3 synthase
-
 Homo sapiens
2.4.3.9 SAT-1
-
 Homo sapiens
2.4.3.9 sialyltransferase-1
-
 Homo sapiens

Expression

EC Number Organism Comment Expression
2.4.3.9 Homo sapiens SAT-1 mRNA level and GM3 synthase activity in vitro are markedly upregulated in 3 SAT-1-transfected clones with respect to wild-type and mock-transfected A2780 cells up

General Information

EC Number General Information Comment Organism
2.4.1.92 physiological function GM3 synthase overexpression results in reduced cell motility accompanied by reduced levels of the epithelial-mesenchymal transition marker alpha smooth muscle actin and in caveolin-1 upregulation in human ovarian carcinoma cells Homo sapiens
2.4.3.9 physiological function overexpression of GM3 synthase in A2780 cells consistently results in elevated ganglioside (GM3, GM2 and GD1a) levels. GM3 synthase overexpressing cells have a growth rate similar to wild-type cells, but show a strongly reduced in vitro cell motility accompanied by reduced levels of the epithelial-mesenchymal transition marker alpha smooth muscle actin. Caveolin-1 is markedly upregulated in GM3 synthase overexpressing cells. Higher levels of the inactive form of c-Src are present in GM3 synthase overexpressing cells, associated with a ganglioside- and caveolin-rich detergent insoluble fraction Homo sapiens