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Literature summary extracted from

  • Cheng, J.; Huang, S.; Yu, H.; Li, Y.; Lau, K.; Chen, X.
    Trans-sialidase activity of Photobacterium damsela alpha2,6-sialyltransferase and its application in the synthesis of sialosides (2010), Glycobiology, 20, 260-268.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.3.1 CMP cytidine 5'-monophosphate, the product of sialyltransferase reactions, is not required by the trans-sialidase activity of the enzyme but enhances the trans-sialidase activity modestly as a non-essential activator. CMP can enhance the efficiency of the alpha2,6-sialidase activity of DELTA15Pd2,6ST in a dose-dependent manner Photobacterium damselae
3.2.1.18 CMP cytidine 5'-monophosphate is not required by the trans-sialidase activity of the enzyme but enhances the trans-sialidase activity modestly as a non-essential activator Photobacterium damselae

Application

EC Number Application Comment Organism
2.4.3.1 synthesis application of the alpha2,6-trans-sialidase activity of DELTA15Pd2,6ST in the synthesis of sialosides Photobacterium damselae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.3.1 CDP at a concentration of 1 mM inhibits both sialidase and trans-sialidase activities of DELTA15Pd2,6ST Photobacterium damselae
2.4.3.1 CTP at a concentration of 1 mM inhibits both sialidase and trans-sialidase activities of DELTA15Pd2,6ST Photobacterium damselae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.3.1 53
-
 Neu5Acalpha-4-nitrophenyl alpha2,6-trans-sialidase activity of DELTA15Pd2,6ST Photobacterium damselae
3.2.1.18 53
-
 4-nitrophenyl alpha-D-N-acetylneuraminic acid
-
 Photobacterium damselae

Organism

EC Number Organism UniProt Comment Textmining
2.4.3.1 Photobacterium damselae
-
-
-
3.2.1.18 Photobacterium damselae
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.3.1 additional information DELTA15Pd2,6ST preferentially cleaves alpha2,6-linked sialic acid Photobacterium damselae ?
-
 ?
2.4.3.1 Neu5Acalpha-4-nitrophenyl + 4-methylumbelliferyl-beta-D-lactoside alpha2,6-trans-sialidase activity of DELTA15Pd2,6ST Photobacterium damselae ?
-
 ?
3.2.1.18 4-nitrophenyl alpha-D-N-acetylneuraminic acid + H2O
-
 Photobacterium damselae ?
-
 ?
3.2.1.18 alpha(2-6)-sialyllactose + H2O DELTA15Pd2,6ST-catalyzed trans-sialidase reactions follow a ping-pong bi-bi reaction mechanism, DELTA15Pd2,6ST preferentially cleaves alpha2,6-linked sialic acid Photobacterium damselae sialic acid + lactose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.3.1 alpha2,6-sialyltransferase
-
 Photobacterium damselae
3.2.1.18 alpha2,6-sialidase
-
 Photobacterium damselae
3.2.1.18 alpha2,6-sialyltransferase
-
 Photobacterium damselae
3.2.1.18 alpha2,6-trans-sialidase
-
 Photobacterium damselae
3.2.1.18 DELTA15Pd2,6ST the enzyme has additional alpha2,6-trans-sialidase and alpha2,6-sialidase activities Photobacterium damselae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.3.1 0.883
-
 Neu5Acalpha-4-nitrophenyl alpha2,6-trans-sialidase activity of DELTA15Pd2,6ST Photobacterium damselae
3.2.1.18 0.88
-
 4-nitrophenyl alpha-D-N-acetylneuraminic acid
-
 Photobacterium damselae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.3.1 5.8
-
 alpha2,6-sialidase and alpha2,6-trans-sialidase activities of DELTA15Pd2,6ST in MES buffer Photobacterium damselae

General Information

EC Number General Information Comment Organism
2.4.3.1 physiological function DELTA15Pd2,6ST-catalyzed trans-sialidase reaction follows a ping-pong bi-bi reaction mechanism. DELTA15Pd2,6ST also has alpha2,6-sialidase activity Photobacterium damselae

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.3.1 0.016
-
 Neu5Acalpha-4-nitrophenyl alpha2,6-trans-sialidase activity of DELTA15Pd2,6ST Photobacterium damselae