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Literature summary extracted from

  • Brumshtein, B.; Salinas, P.; Peterson, B.; Chan, V.; Silman, I.; Sussman, J.L.; Savickas, P.J.; Robinson, G.S.; Futerman, A.H.
    Characterization of gene-activated human acid-beta-glucosidase: Crystal structure, glycan composition, and internalization into macrophages (2010), Glycobiology, 20, 24-32.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.45 expressed in Chinese hamster ovary cells and in transgenic carrot cells Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.45 velaglucerase alfa, micro-batch crystallization under oil, using 1 M (NH4)2SO4/0.1 M HEPES, pH 7.0, containing 0.5% (w/v) PEG8000 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.45 0.015
-
 glucosylceramide imiglucerase, in 50 mM sodium citrate, pH 6.0 with 0.75 mg/ml bovine serum albumin, at 37°C Homo sapiens
3.2.1.45 0.019
-
 glucosylceramide velaglucerase alfa, in 50 mM sodium citrate, pH 6.0 with 0.75 mg/ml bovine serum albumin, at 37°C Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.45 Homo sapiens P04062
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.45
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.45 glucosylceramide + H2O
-
 Homo sapiens beta-D-glucose + ceramide
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.45 acid-beta-glucocerebrosidase
-
 Homo sapiens
3.2.1.45 acid-beta-glucosidase
-
 Homo sapiens
3.2.1.45 Cerezyme commercial preparation Homo sapiens
3.2.1.45 gene-activated human GlcCerase commercial preparation Homo sapiens
3.2.1.45 GlcCerase
-
 Homo sapiens
3.2.1.45 Imiglucerase
-
 Homo sapiens
3.2.1.45 velaglucerase alfa commercial preparation Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.45 31.67
-
 glucosylceramide imiglucerase alfa, in 50 mM sodium citrate, pH 6.0 with 0.75 mg/ml bovine serum albumin, at 37°C Homo sapiens
3.2.1.45 35
-
 glucosylceramide velaglucerase alfa, in 50 mM sodium citrate, pH 6.0 with 0.75 mg/ml bovine serum albumin, at 37°C Homo sapiens