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Literature summary extracted from

  • Condac, E.; Dale, G.L.; Bender-Neal, D.; Ferencz, B.; Towner, R.; Hinsdale, M.E.
    Xylosyltransferase II is a significant contributor of circulating xylosyltransferase levels and platelets constitute an important source of xylosyltransferase in serum (2009), Glycobiology, 19, 829-833.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.2.26 additional information unactivated platelets harbor significant XylT activity that is released upon activation with thrombin. Diseases affecting platelet activation may alter serum total XylT activity. In vivo liver cells may contribute significant XylT activity to circulating levels Homo sapiens

Application

EC Number Application Comment Organism
2.4.2.26 medicine serum XylT levels may be an informative biomarker in patients who suffer from diseases affecting platelet and/or liver homeostasis Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.26 c-Myc/TGFalpha doubly transgenic mice. Modified minigene Xylt2 expression construct producing a protein lacking the 45 N-terminal amino acids including the transmembrane domains. The Xylt1 expression cassette producing a protein that lacks 94 N-terminal amino acids including the transmembrane domain which is predicted as for XylT2. Both Xylt1 and Xylt2 expression cassettes cloned into a modified pcDNA3.1 vector containing a transferrin signal peptide and an HPC4 epitope tag in frame on the N-terminus Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.26 additional information reduced total XylT activity in sera from mice possessing visible tumors as compared to controls Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.26 0.01172
-
 QEEEGSGGGQKK in Xylt2 Mus musculus
2.4.2.26 0.02048
-
 QEEEGSGGGQKK in Xylt1 Mus musculus
2.4.2.26 0.2132
-
 TENEGSGLTNIK in Xylt1 Mus musculus
2.4.2.26 0.3905
-
 TENEGSGLTNIK in Xylt2 Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.26 Homo sapiens
-
-
-
2.4.2.26 Mus musculus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.2.26 blood plasma
-
 Homo sapiens
-
2.4.2.26 blood plasma XylT levels in plasma are approximately 200% lower than those in serum due in part to XylT released by platelets during blood clotting in vitro. In Xylt2+/+ mice, total serum XylT activity is 42% higher than in plasma. In Xylt2-/- mice, no significant difference between serum and plasma total XylT activity Mus musculus
-
2.4.2.26 blood platelet unactivated platelets harbor significant XylT activity that is released upon activation with thrombin Homo sapiens
-
2.4.2.26 blood serum XylT2 is predominant Homo sapiens
-
2.4.2.26 blood serum XylT2 is predominant. XylT levels in serum are approximately 200% higher than those in plasma due in part to XylT released by platelets during blood clotting in vitro. In Xylt2+/+ mice, total serum XylT activity is 42% higher than in plasma. In Xylt2-/- mice, no significant difference between serum and plasma total XylT activity Mus musculus
-
2.4.2.26 Hep-G2 cell
-
 Homo sapiens
-
2.4.2.26 liver liver is a significant source of circulating XylT2 activity Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.26 UDP-D-xylose + QEEEGSGGGQKK QEEEGSGGGQKK is a better acceptor substrate than TENEGSGLTNIK Mus musculus UDP + QEEEG-(D-xylosyl)SGGGQKK
-
 ?
2.4.2.26 UDP-D-xylose + QEEEGSGGGQKK the bikunin nuclear acceptor peptide QEEEGSGGGQKK is not a differential acceptor substrate for the human XylT isoenzymes but is a good acceptor substrate for total XylT activity measurements Homo sapiens UDP + QEEEG-(D-xylosyl)SGGGQKK
-
 ?
2.4.2.26 UDP-D-xylose + TENEGSGLTNIK QEEEGSGGGQKK is a better acceptor substrate than TENEGSGLTNIK Mus musculus UDP + TENEG-(D-xylosyl)SGLTNIK
-
 ?
2.4.2.26 UDP-D-xylose + TENEGSGLTNIK the 3-A4-amyloid protein precursor protein peptide TENEGSGLTNIK is an acceptor substrate for XylT1 Homo sapiens UDP + TENEG-(D-xylosyl)SGLTNIK
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.2.26 xylosyltransferase I
-
 Mus musculus
2.4.2.26 xylosyltransferase I
-
 Homo sapiens
2.4.2.26 xylosyltransferase II
-
 Mus musculus
2.4.2.26 xylosyltransferase II
-
 Homo sapiens
2.4.2.26 XYLT1
-
 Mus musculus
2.4.2.26 XYLT1
-
 Homo sapiens
2.4.2.26 XYLT2
-
 Mus musculus
2.4.2.26 XYLT2
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
2.4.2.26 physiological function in Xylt2-/- mice total XylT activity is decreased ca. 99% as compared to Xylt2+/+ mice Mus musculus