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Literature summary extracted from

  • Ishibashi, Y.; Nagamatsu, Y.; Meyer, S.; Imamura, A.; Ishida, H.; Kiso, M.; Okino, N.; Geyer, R.; Ito, M.
    Transglycosylation-based fluorescent labeling of 6-gala series glycolipids by endogalactosylceramidase (2009), Glycobiology, 19, 797-807.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.123 expressed in Escherichia coli BL21(DE3) cells Rhodococcus equi

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.123 Rhodococcus equi
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.123 Ni Sepharose 6 column chromatography and Superdex 200 gel filtration Rhodococcus equi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.123 7-nitro-2,1,3-benzoxadiazole-pentanol + digalactosyldiacylglycerol transglycosylation Rhodococcus equi ?
-
 ?
3.2.1.123 Galalpha1-6Galbeta1-3-diacylglycerol + H2O
-
 Rhodococcus equi ?
-
 ?
3.2.1.123 Galbeta1-1'-ceramide + H2O
-
 Rhodococcus equi ?
-
 ?
3.2.1.123 Galbeta1-6Galbeta1-1'-ceramide + H2O highest transglycosylation efficiency Rhodococcus equi ?
-
 ?
3.2.1.123 Galbeta1-6Galbeta1-6Galbeta1-1'-ceramide + H2O
-
 Rhodococcus equi ?
-
 ?
3.2.1.123 Galbeta1-6Galbeta1-6Galbeta1-6Galbeta1-1'-ceramide + H2O
-
 Rhodococcus equi ?
-
 ?
3.2.1.123 Glcbeta1-6Galbeta1-1'-ceramide + H2O
-
 Rhodococcus equi ?
-
 ?
3.2.1.123 additional information EGALC specifically hydrolyzes the Galbeta1-1'-ceramide linkage in 6-Gal series glycosphingolipids Rhodococcus equi ?
-
 ?
3.2.1.123 additional information no activity towards Galbeta1-6Glcbeta1-1'-ceramide, Galbeta1-4Galbeta1-1'-ceramide, Galalpha1-1'ceramide, Glcbeta1-1'-ceramide, Galbeta1-4Glcbeta1-1'ceramide, Galalpha1-4Galbeta1-4Glcbeta1-1'-ceramide, and Galbeta1-3GalNAcbeta1-4(NeuAcalpha2-3)Galbeta1-4Glcbeta1-1'-ceramide Rhodococcus equi ?
-
 ?
3.2.1.123 trigalactosylceramide + H2O
-
 Rhodococcus equi ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.123 EGALC
-
 Rhodococcus equi
3.2.1.123 endogalactosylceramidase
-
 Rhodococcus equi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.123 37
-
 optimal temperature for transglycosylation by EGALC Rhodococcus equi