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Literature summary extracted from

  • Soya, N.; Shoemaker, G.K.; Palcic, M.M.; Klassen, J.S.
    Comparative study of substrate and product binding to the human ABO(H) blood group glycosyltransferases (2009), Glycobiology, 19, 1224-1234.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.37 overexpression of truncated GTB in Escherichia coli strain BL21 Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.37 additional information construction of truncated GTB monomers composed of the full C-terminal and catalytic domain as well as a truncated N-terminal domain Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.37 additional information
-
additional information kinetic mechanism and thermodynamic analysis of GTB using electrospray ionization mass spectrometry, comparison to GTA, EC 2.4.1.40 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.37 Mn2+ required, in the absence of divalent metal ion, GTB does not exhibit any appreciable affinity for its native donor UDP-Gal Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.37 34483
-
x * 34483, recombinant truncated enzyme mutant, mass spectrometry Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.37 UDP-galactose + alpha-L-fucosyl-(1,2)-D-galactose Homo sapiens
-
UDP + alpha-D-galactosyl-(1,3)-[alpha-L-fucosyl(1,2)]-D-galactose
-
?
2.4.1.37 UDP-galactose + blood group antigen H Homo sapiens
-
UDP + alpha-D-galactosyl-blood group antigen H
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.37 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.37 additional information enzyme-substrate interaction analysis by electrospray ionization mass spectrometry, binding of substrate and product analogues, overview Homo sapiens ?
-
?
2.4.1.37 UDP-galactose + alpha-L-fucosyl-(1,2)-D-galactose
-
Homo sapiens UDP + alpha-D-galactosyl-(1,3)-[alpha-L-fucosyl(1,2)]-D-galactose
-
?
2.4.1.37 UDP-galactose + alpha-L-fucosyl-(1,2)-D-galactose i.e. H disaccharide Homo sapiens UDP + alpha-D-galactosyl-(1,3)-[alpha-L-fucosyl(1,2)]-D-galactose in the absence of UDP and Mn2+, GTB recognizes its trisaccharide product with a low affinity of about 1 mM, while the presence of UDP and Mn2+ precludes B-trisaccharide binding ?
2.4.1.37 UDP-galactose + blood group antigen H
-
Homo sapiens UDP + alpha-D-galactosyl-blood group antigen H
-
?
2.4.1.37 UDP-galactose + blood group antigen H GTB catalyzes the transfer of galactose from UDP-Gal to the C3 position of the terminal galactose of H antigen acceptors Homo sapiens UDP + alpha-D-galactosyl-blood group antigen H
-
?

Subunits

EC Number Subunits Comment Organism
2.4.1.37 ? x * 34483, recombinant truncated enzyme mutant, mass spectrometry Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.4.1.37 alpha-(1,3)-galactosyltransferase
-
Homo sapiens
2.4.1.37 GTB
-
Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.37 24
-
assay at Homo sapiens