BRENDA - Enzyme Database

Electron paramagnetic resonance (EPR) spectroscopy of the stable-free radical in the native metallo-cofactor of the manganese-ribonucleotide reductase (Mn-RNR) of Corynebacterium glutamicum

Abbouni, B.; Oehlmann, W.; Stolle, P.; Pierik, A.J.; Auling, G.; Free Radic. Res. 43, 943-950 (2009)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.17.4.1
dATP
positive effector
Corynebacterium glutamicum
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.17.4.1
4-methoxyphenol
-
Corynebacterium glutamicum
1.17.4.1
EDTA
-
Corynebacterium glutamicum
1.17.4.1
Hydroxyurea
-
Corynebacterium glutamicum
1.17.4.1
additional information
the enzyme is inhibited by radical scavengers
Corynebacterium glutamicum
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.17.4.1
Fe2+
the enzyme contains only 0.06 mol iron per mol of R2F subunit
Corynebacterium glutamicum
1.17.4.1
Mn2+
0.8 mol manganese per mol of R2F subunit, oragnized in a coupled binuclear centre with paramagnetic ground state or in a weakly coupled binuclear centre with therminally populated paramagnetic excites state which appears as binuclear Mn2+, overview
Corynebacterium glutamicum
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.17.4.1
34000
-
x * 34000, R2F subunit, DSD-PAGE
Corynebacterium glutamicum
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.17.4.1
Corynebacterium glutamicum
-
gene nrdF encoding subunit R2F
-
1.17.4.1
Corynebacterium glutamicum R163
-
gene nrdF encoding subunit R2F
-
Purification (Commentary)
EC Number
Commentary
Organism
1.17.4.1
native R2F subunit by 2',5'-ADP affinity and hydrophobic interaction chromatography, followed by two different steps of gel filtration, an further by anion exchange chromnatography for metallo-cofactor analysis
Corynebacterium glutamicum
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.17.4.1
0.53
-
subunit R2F
Corynebacterium glutamicum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.17.4.1
additional information
CDP as substrate
703813
Corynebacterium glutamicum
?
-
-
-
-
1.17.4.1
additional information
CDP as substrate
703813
Corynebacterium glutamicum R163
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
1.17.4.1
?
x * 34000, R2F subunit, DSD-PAGE
Corynebacterium glutamicum
1.17.4.1
More
hybrid holoenzyme, consisting of the small manganese-containing R2F subunit and the large catalytic subunit R1E
Corynebacterium glutamicum
IC50 Value
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
1.17.4.1
0.5
-
-
Corynebacterium glutamicum
4-methoxyphenol
1.17.4.1
1.7
-
-
Corynebacterium glutamicum
Hydroxyurea
1.17.4.1
2.4
-
-
Corynebacterium glutamicum
EDTA
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.17.4.1
dATP
positive effector
Corynebacterium glutamicum
IC50 Value (protein specific)
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
1.17.4.1
0.5
-
-
Corynebacterium glutamicum
4-methoxyphenol
1.17.4.1
1.7
-
-
Corynebacterium glutamicum
Hydroxyurea
1.17.4.1
2.4
-
-
Corynebacterium glutamicum
EDTA
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.17.4.1
4-methoxyphenol
-
Corynebacterium glutamicum
1.17.4.1
EDTA
-
Corynebacterium glutamicum
1.17.4.1
Hydroxyurea
-
Corynebacterium glutamicum
1.17.4.1
additional information
the enzyme is inhibited by radical scavengers
Corynebacterium glutamicum
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.17.4.1
Fe2+
the enzyme contains only 0.06 mol iron per mol of R2F subunit
Corynebacterium glutamicum
1.17.4.1
Mn2+
0.8 mol manganese per mol of R2F subunit, oragnized in a coupled binuclear centre with paramagnetic ground state or in a weakly coupled binuclear centre with therminally populated paramagnetic excites state which appears as binuclear Mn2+, overview
Corynebacterium glutamicum
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.17.4.1
34000
-
x * 34000, R2F subunit, DSD-PAGE
Corynebacterium glutamicum
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.17.4.1
native R2F subunit by 2',5'-ADP affinity and hydrophobic interaction chromatography, followed by two different steps of gel filtration, an further by anion exchange chromnatography for metallo-cofactor analysis
Corynebacterium glutamicum
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.17.4.1
0.53
-
subunit R2F
Corynebacterium glutamicum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.17.4.1
additional information
CDP as substrate
703813
Corynebacterium glutamicum
?
-
-
-
-
1.17.4.1
additional information
CDP as substrate
703813
Corynebacterium glutamicum R163
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.17.4.1
?
x * 34000, R2F subunit, DSD-PAGE
Corynebacterium glutamicum
1.17.4.1
More
hybrid holoenzyme, consisting of the small manganese-containing R2F subunit and the large catalytic subunit R1E
Corynebacterium glutamicum