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Literature summary extracted from

  • Kaur, S.; Mishra, M.N.; Tripathi, A.K.
    Regulation of expression and biochemical characterization of a beta-class carbonic anhydrase from the plant growth-promoting rhizobacterium, Azospirillum brasilense Sp7 (2009), FEMS Microbiol. Lett., 299, 149-158.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.1 expressed in Escherichia coli BL21(DE3) cells Azospirillum brasilense

General Stability

EC Number General Stability Organism
4.2.1.1 the purified protein is unstable in the absence of glycerol and aggregates irreversibly Azospirillum brasilense

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.1 acetazolamide
-
Azospirillum brasilense
4.2.1.1 azide
-
Azospirillum brasilense
4.2.1.1 Iodide
-
Azospirillum brasilense
4.2.1.1 nitrate
-
Azospirillum brasilense
4.2.1.1 sulfonamide
-
Azospirillum brasilense

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.1 29000
-
4 * 29000, SDS-PAGE, the native recombinant enzyme is a homotetramer Azospirillum brasilense
4.2.1.1 120000
-
apparent molecular mass of the purified protein, gel filtration Azospirillum brasilense

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.1 Azospirillum brasilense
-
-
-
4.2.1.1 Azospirillum brasilense Sp7
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.1 Ni-NTA resin column chromatography Azospirillum brasilense

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.1 CO2 + H2O
-
Azospirillum brasilense H2CO3
-
r
4.2.1.1 CO2 + H2O
-
Azospirillum brasilense Sp7 H2CO3
-
r

Subunits

EC Number Subunits Comment Organism
4.2.1.1 homotetramer 4 * 29000, SDS-PAGE, the native recombinant enzyme is a homotetramer Azospirillum brasilense

Synonyms

EC Number Synonyms Comment Organism
4.2.1.1 beta-class carbonic anhydrase
-
Azospirillum brasilense

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
4.2.1.1 0.008
-
-
Azospirillum brasilense acetazolamide
4.2.1.1 0.014
-
-
Azospirillum brasilense azide
4.2.1.1 0.073
-
-
Azospirillum brasilense nitrate
4.2.1.1 0.15
-
-
Azospirillum brasilense Iodide

General Information

EC Number General Information Comment Organism
4.2.1.1 physiological function beta carbonic anhydrase is required for scavenging CO2 from the ambient air Azospirillum brasilense