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Literature summary extracted from

  • Kang, H.K.; Oh, J.S.; Kim, D.
    Molecular characterization and expression analysis of the glucansucrase DSRWC from Weissella cibaria synthesizing a alpha(1-6) glucan (2009), FEMS Microbiol. Lett., 292, 33-41.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.5 gene dsrWC, DNA and amino acid sequence determination and analysis, cloning in Escherichia coli strain Top10F' and expression in Escherichia coli strain BL21(DE3) Weissella cibaria

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.5 Ca2+
-
 Weissella cibaria

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.5 161998
-
 x * 161998, sequence calculation Weissella cibaria

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.5 sucrose + [(1,6)-alpha-D-glucosyl]n Weissella cibaria
-
 D-fructose + [(1,6)-alpha-D-glucosyl]n+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.5 Weissella cibaria B9UNL6 isolated from human saliva, gene dsrWC
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.5 recombinant enzyme 5.1fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and ultrafiltration Weissella cibaria

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.5 additional information the recombinant DSRWC synthesizes oligosaccharides in the presence of maltose or isomaltose as an acceptor and the synthesized products include alpha-1,6-linked glucosyl residues in addition to the maltosyl or isomaltosyl residue. rDSRWC synthesizes water-soluble polymers using sucrose as substrate, and rDSRBWC synthesizes dextran and leucrose [alpha-D-glucopyranosyl-(1,5)-beta-D-fructofuranose] Weissella cibaria ?
-
 ?
2.4.1.5 sucrose + [(1,6)-alpha-D-glucosyl]n
-
 Weissella cibaria D-fructose + [(1,6)-alpha-D-glucosyl]n+1
-
 ?
2.4.1.5 sucrose + [(1,6)-alpha-D-glucosyl]n In the presence of maltose, the most abundant reaction product is panose, i.e. MP1 or alpha-D-glucopyranosyl-(1,6)-alpha-D-glucopyranosyl-(1,4)-D-glucopyranose, with 35.3%, detailed NMR product identification and structure analysis, overview Weissella cibaria D-fructose + [(1,6)-alpha-D-glucosyl]n+1
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.5 ? x * 161998, sequence calculation Weissella cibaria

Synonyms

EC Number Synonyms Comment Organism
2.4.1.5 DSRWC
-
 Weissella cibaria
2.4.1.5 glucansucrase
-
 Weissella cibaria

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.5 30
-
-
 Weissella cibaria

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.5 20 50 62% at 20°C, activity range Weissella cibaria

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.5 5.2
-
-
 Weissella cibaria

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.5 3.5 8 activity range Weissella cibaria

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.5 5 6 over 80% of maximal activity within this range, but the activity decreases rapidly outside this pH range Weissella cibaria