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Literature summary extracted from

  • Reshetnikov, A.S.; Rozova, O.N.; Khmelenina, V.N.; Mustakhimov, I.I.; Beschastny, A.P.; Murrell, J.C.; Trotsenko, Y.A.
    Characterization of the pyrophosphate-dependent 6-phosphofructokinase from Methylococcus capsulatus Bath (2008), FEMS Microbiol. Lett., 288, 202-210.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.90 expressed in Escherichia coli BL21(DE3) cells Methylococcus capsulatus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.90 additional information addition of citrate, pyruvate, phosphoenolpyruvate, L-serine, phosphoglycerate, ATP, ADP, AMP, NADP, NADs and NADPH to the assay mixture has no effect on activity Methylococcus capsulatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.90 0.027
-
sedoheptulose 7-phosphate in 0.1 M HEPES-HCl, pH 7.0, at 30°C Methylococcus capsulatus
2.7.1.90 0.027
-
diphosphate using D-fructose 6-phosphate as cosubstrate, in 0.1 M HEPES-HCl, pH 7.0, at 30°C Methylococcus capsulatus
2.7.1.90 0.328
-
D-fructose 1,6-bisphosphate in 0.1 M HEPES-HCl, pH 7.0, at 30°C Methylococcus capsulatus
2.7.1.90 2.27
-
D-fructose 6-phosphate in 0.1 M HEPES-HCl, pH 7.0, at 30°C Methylococcus capsulatus
2.7.1.90 8.69
-
phosphate using D-fructose 1,6-bisphosphate as cosubstrate, in 0.1 M HEPES-HCl, pH 7.0, at 30°C Methylococcus capsulatus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.90 Mg2+ required for maximal activity, Co2+ or Mn2+ can substitute for Mg2+ with 63% and 48% of maximal activity Methylococcus capsulatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.90 45000
-
2 * 45000, SDS-PAGE Methylococcus capsulatus
2.7.1.90 90000
-
gel filtration Methylococcus capsulatus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.90 Methylococcus capsulatus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.90 Ni-NTA agarose column chromatography Methylococcus capsulatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.90 diphosphate + D-fructose 6-phosphate strong specificity for diphosphate as a phosphoryl donor Methylococcus capsulatus phosphate + D-fructose 1,6-bisphosphate
-
r
2.7.1.90 diphosphate + D-ribulose 5-phosphate
-
Methylococcus capsulatus phosphate + ?
-
?
2.7.1.90 diphosphate + sedoheptulose 7-phosphate
-
Methylococcus capsulatus phosphate + ?
-
?
2.7.1.90 additional information ATP, ADP, AMP, CTP, CDP, CMP, UTP, UDP, UMP, GTP, GDP, and GMP at 1 mM as well as polyphosphates (n=3, n=9, n=15 and n=45) at 0.1 mg/ml cannot substitute for diphosphate, D-glucose 6-phosphate, D-glucose 1-phosphate, D-fructose 1-phosphate, D-mannose 6-phosphate, D-ribose 5-phosphate, D-ribose 1-phosphate, or D-erythrose 4-phosphate cannot substitute for D-fructose 6-phosphate as a phosphate acceptor Methylococcus capsulatus ?
-
?

Subunits

EC Number Subunits Comment Organism
2.7.1.90 homodimer 2 * 45000, SDS-PAGE Methylococcus capsulatus

Synonyms

EC Number Synonyms Comment Organism
2.7.1.90 PPi-PFK
-
Methylococcus capsulatus
2.7.1.90 pyrophosphate-dependent 6-phosphofructokinase
-
Methylococcus capsulatus
2.7.1.90 pyrophosphate-dependent fructose-6-phosphate 1-phosphotransferase
-
Methylococcus capsulatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.90 30
-
-
Methylococcus capsulatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.90 7
-
-
Methylococcus capsulatus