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Literature summary extracted from

  • Pilet, E.; Nicolet, Y.; Mathevon, C.; Douki, T.; Fontecilla-Camps, J.C.; Fontecave, M.
    The role of the maturase HydG in [FeFe]-hydrogenase active site synthesis and assembly (2009), FEBS Lett., 583, 506-511.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.12.99.6 HydE can coordinate variable amounts of Fe and sulfide Thermotoga maritima
1.12.99.6 HydE is required to synthesize and assemble the active site utilizing Escherichia coli metabolites Clostridium acetobutylicum
1.12.99.6 HydEF maturase, role in biosynthesis of the active site. HydEF and hydG, coexpressed with the structural genes hydA1 and hydA2 in Escherichia coli lead to active [FeFe]-hydrogenase Chlamydomonas reinhardtii
1.12.99.6 HydF
-
Thermotoga maritima
1.12.99.6 HydF is required to synthesize and assemble the active site utilizing Escherichia coli metabolites Clostridium acetobutylicum
1.12.99.6 HydG is required to synthesize and assemble the active site utilizing Escherichia coli metabolites Clostridium acetobutylicum
1.12.99.6 HydG maturase, role in biosynthesis of the active site Desulfovibrio vulgaris
1.12.99.6 HydG maturase, role in biosynthesis of the active site. HydEF and hydG, coexpressed with the structural genes hydA1 and hydA2 in Escherichia coli lead to active [FeFe]-hydrogenase Chlamydomonas reinhardtii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.12.99.6 expressed in Escherichia coli Desulfovibrio vulgaris
1.12.99.6 hydA1 and hydA2 expressed in Escherichia coli Chlamydomonas reinhardtii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.12.99.6 Fe lacks the ferredoxin-like clusters, but contains the H-cluster [Fe4S4] component Chlamydomonas reinhardtii
1.12.99.6 Fe two ferredoxin-like [Fe4S4] clusters Desulfovibrio vulgaris
1.12.99.6 Fe [FeS] cluster Thermotoga maritima

Organism

EC Number Organism UniProt Comment Textmining
1.12.99.6 Chlamydomonas reinhardtii
-
-
-
1.12.99.6 Clostridium acetobutylicum
-
-
-
1.12.99.6 Desulfovibrio vulgaris
-
-
-
1.12.99.6 Thermotoga maritima
-
-
-

Synonyms

EC Number Synonyms Comment Organism
1.12.99.6 HydA1
-
Chlamydomonas reinhardtii
1.12.99.6 HydA2
-
Chlamydomonas reinhardtii
1.12.99.6 [FeFe]-hydrogenase
-
Chlamydomonas reinhardtii
1.12.99.6 [FeFe]-hydrogenase
-
Clostridium acetobutylicum
1.12.99.6 [FeFe]-hydrogenase
-
Desulfovibrio vulgaris
1.12.99.6 [FeFe]-hydrogenase
-
Thermotoga maritima

General Information

EC Number General Information Comment Organism
1.12.99.6 malfunction heterologous expression of the two structural genes of [FeFe]-hydrogenase in Escherichia coli yields an inactive enzyme, only the two ferredoxin-like [Fe4S4] clusters are unambiguously assembled Desulfovibrio vulgaris