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Literature summary extracted from

  • Florio, R.; Chiaraluce, R.; Consalvi, V.; Paiardini, A.; Catacchio, B.; Bossa, F.; Contestabile, R.
    Structural stability of the cofactor binding site in Escherichia coli serine hydroxymethyltransferase - the role of evolutionarily conserved hydrophobic contacts (2009), FEBS J., 276, 7319-7328.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.1.2.1 L276A the mutation has the effect of lowering the cooperativity of urea denaturation process Escherichia coli
2.1.2.1 L785A/L276A the mutation has the effect of lowering the cooperativity of urea denaturation process Escherichia coli
2.1.2.1 L85A the mutation affect the quaternary structure stability of SHMT Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.2.1 Urea in 1 M urea, almost all the cofactor is bound to the enzyme as internal aldimine, indicating that the loss of activity does not result from the denaturation of the active site, these observations suggest that urea might act as an enzyme inhibitor Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.2.1 91000
-
 SDS-PAGE Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.1 Escherichia coli P0A825
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.2.1 DEAE-Sepharose column chromatography and Phenyl-Sepharose column chromatography Escherichia coli

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.1.2.1 denatured SHMT samples (0.023 mM in 8 M urea at 20°C) are able to recover after 4 h when kept with 8 M urea in 50 mM Na-HEPES, pH 7.2, containing 0.2 mM dithiothreitol and 0.1 mM EDTA for 15 h at 20°C Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.1 L-serine + tetrahydrofolate
-
 Escherichia coli glycine + 5,10-methylenetetrahydrofolate + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.2.1 serine hydroxymethyltransferase
-
 Escherichia coli
2.1.2.1 SHMT
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.2.1 pyridoxal 5'-phosphate
-
 Escherichia coli